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Microbial biomass, microbial diversity, soil carbon storage, and stability after incubation of soil from grass–clover pastures of different age

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Abstract

A laboratory incubation study with clover grass pasture soils of seven different ages (0, 1, 2, 3, 4, 5, and 16 production years) was carried out to determine initial soil carbon (C) and nitrogen (N) stocks and potentials for greenhouse gas emissions (N2O and CO2). Compared with the soil from the recently established pasture, an increase of total soil C and N was observed along with pasture age. Greenhouse gas emissions were low and not significantly different among the soils from younger pastures (0–5 years), but especially N2O emissions increased markedly in the soil from 16-year-old grass–clover. Low emissions might mainly be due to an early C limitation occurring in the soils from younger pastures, which was also corroborated by decreasing levels of cold water-extractable C and early shifts within the microbial community. However, higher emissions from the old pasture soil were offset by its increase in total soil C. A longer ley phase without soil disturbance may therefore be beneficial in terms of overall C sequestration in systems with temporary grass–clover swards.

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Acknowledgments

The authors thank Nina Wiese Thomsen, Anja Nielsen, and Anne Grethe Holm-Jensen for valuable technical assistance. The advice of Mette Sustmann Carter regarding N2O measurements is gratefully acknowledged.

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Authors and Affiliations

  1. Risø National Laboratory for Sustainable Energy, Biosystems Division, Technical University of Denmark, Frederiksborgvej 399, P.O. Box 49, 4000, Roskilde, Denmark

    Dorette Müller-Stöver, Henrik Hauggaard-Nielsen & Per Ambus

  2. Faculty of Agricultural Sciences, Department of Agroecology and Environment, Aarhus University, Blichers Allé 20, 8830, Tjele, Denmark

    Jørgen Eriksen

  3. National Environmental Research Institute, Department of Environmental Chemistry and Microbiology, Aarhus University, Frederiksborgvej 399, 4000, Roskilde, Denmark

    Anders Johansen

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  1. Dorette Müller-Stöver
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Correspondence to Dorette Müller-Stöver.

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Müller-Stöver, D., Hauggaard-Nielsen, H., Eriksen, J. et al. Microbial biomass, microbial diversity, soil carbon storage, and stability after incubation of soil from grass–clover pastures of different age. Biol Fertil Soils 48, 371–383 (2012). https://doi.org/10.1007/s00374-011-0633-6

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  • DOI: https://doi.org/10.1007/s00374-011-0633-6

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