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Heterotrophic nitrification is the predominant NO −3 production mechanism in coniferous but not broad-leaf acid forest soil in subtropical China

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Abstract

A study was carried out to investigate the potential gross nitrogen (N) transformations in natural secondary coniferous and evergreen broad-leaf forest soils in subtropical China. The simultaneously occurring gross N transformations in soil were quantified by a 15N tracing study. The results showed that N dynamics were dominated by NH +4 turnover in both soils. The total mineralization (from labile and recalcitrant organic N) in the broad-leaf forest was more than twice the rate in the coniferous forest soil. The total rate of mineral N production (NH +4  + NO −3 ) from the large recalcitrant organic N pool was similar in the two forest soils. However, appreciable NO −3 production was only observed in the coniferous forest soil due to heterotrophic nitrification (i.e. direct oxidation of organic N to NO −3 ), whereas nitrification in broad-leaf forest was little (or negligible). Thus, a distinct shift occurred from predominantly NH +4 production in the broad-leaf forest soil to a balanced production of NH +4 and NO −3 in the coniferous forest soil. This may be a mechanism to ensure an adequate supply of available mineral N in the coniferous forest soil and most likely reflects differences in microbial community patterns (possibly saprophytic, fungal, activities in coniferous soils). We show for the first time that the high nitrification rate in these soils may be of heterotrophic rather than autotrophic nature. Furthermore, high NO −3 production was only apparent in the coniferous but not in broad-leaf forest soil. This highlights the association of vegetation type with the size and the activity of the SOM pools that ultimately determines whether only NH +4 or also a high NO −3 turnover is present.

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Acknowledgements

This work is founded by Projects of National Natural Science Foundation of China (40830531 and 40621001) and CAS Knowledge Innovation Program (ISSASIP0702).

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Authors and Affiliations

  1. State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China

    Jinbo Zhang, Tongbin Zhu, Yi Cheng & Zucong Cai

  2. Department of Plant Ecology, Justus-Liebig University Giessen, Heinrich-Buff-Ring 26, 35392, Giessen, Germany

    Christoph Müller

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  1. Jinbo Zhang
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Correspondence to Zucong Cai.

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Zhang, J., Müller, C., Zhu, T. et al. Heterotrophic nitrification is the predominant NO −3 production mechanism in coniferous but not broad-leaf acid forest soil in subtropical China. Biol Fertil Soils 47, 533–542 (2011). https://doi.org/10.1007/s00374-011-0567-z

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