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Effects of pH, ionic strength, and solutes on DNA adsorption by andosols

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Abstract

The adsorption of DNA by two andosols was investigated as function of solution pH, ionic strength in solution and some solutes, so as to understand the behavior of extracellular DNA molecules in two andosols. DNA adsorptions greatly decreased by increasing pH of suspensions in a pH range of 3.0–9.0. The adsorption of DNA molecules by both andosols was not affected by ionic strength from 0.1 to 0.5 mol L−1 NaCl. However, the DNA adsorption increased proportionally by increasing Mg2+ concentration in the suspension. Addition of phosphate decreased DNA adsorption, indicating competition between DNA molecules and phosphate ion in the adsorption by andosols. These results suggest that there are several DNA adsorption mechanisms in soil.

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References

  • Cai P, Huang Q, Zhang X (2006) Microcalorimetrc studies of the effects of MgCl2 concentrations and pH on the adsorption of DNA on montmorillonite, kaolinite and goethite. Appl Clay Sci 32:147–152

    Article  CAS  Google Scholar 

  • Crecchio C, Stotzky G (1998) Binding of DNA on humic acids: effect on transformation of Bacillus subtilis and resistance to DNase. Soil Biol Biochem 30:1061–1067

    Article  CAS  Google Scholar 

  • European Food Safety Authority (EFSA) (2009) Consolidated presentation of the joint Scientific Opinion of the GMO and BIOHAZ panels on the "Use of Antibiotic Resistance Genes as Marker Genes in Genetically Modified Plants" and the scientific opinion of the GMO panel on "Consequences of the Opinion on the Use of Antibiotic Resistance Genes as Marker Genes in Genetically Modified Plants on Previous EFSA Assessments of Individual GM Plants" http://www.efsa.europa.eu/EFSA/efsa_locale-1178620753812_1211902604575.htm

  • Gallori E, Bazzicalupo M, Canto D, Fani R, Nannipieri P, Vettori C, Stotzky G (1994) Transformation of Bacillus subtilis by DNA bound on clay in non-sterile soil. FEMS Microbiol Ecol 15:119–126

    Article  CAS  Google Scholar 

  • Goring CAI, Bartholomew WV (1952) Adsorption of monocleotides, nucleic acids and nucleoproteins by clays. Soil Sci 74:149–164

    Article  CAS  Google Scholar 

  • Greaves MP, Wilson MJ (1969) The adsorption of nucleic acids by montmorillonite. Soil Biol Biochem 1:317–323

    Article  CAS  Google Scholar 

  • Hashizume H, Theng BKG (2007) Adenine, adenosine, ribose and 5′-AMP adsorption to allophone. Clays Clay Minerals 55:599–605

    Article  CAS  Google Scholar 

  • Hingston FJ, Posner AM, Quirk JP (1972) Anion adsorption by goethite and gibbsite. J Soil Sci 23:177–192

    Article  CAS  Google Scholar 

  • Howeler M, Chiorse WC, Walker LP (2003) A quantitative analysis of DNA extraction and purification from compost. J Microbiol Methods 54:37–45

    Article  CAS  PubMed  Google Scholar 

  • Khanna M, Stotzky G (1992) Transformation of Bacillus subtilis by DNA bound on montmorillonite and effect of DNase on the transforming ability of bound DNA. Appl Environ Microbiol 58:930–1939

    Google Scholar 

  • Levy-Booth DJ, Campbell RG, Gulden RH, Hart MM, Powell JR, Klironomos JN, Pauls KP, Swanton CJ, Trevors JT, Dunfield KE (2007) Cycling of extracellular DNA in the soil environment. Soil Biol Biochem 39:2977–2991

    Article  CAS  Google Scholar 

  • Lorenz MG, Wackernagel W (1987) Adsorption of DNA to sand and variable degradation rates of adsorbed DNA. Appl Environ Microbiol 53:2948–2952

    CAS  PubMed  Google Scholar 

  • Lorenz MG, Wackernagel W (1994) Bacterial gene transfer by natural genetic transformation in the environment. Microbiol Rev 58:563–602

    CAS  PubMed  Google Scholar 

  • Marion GM, Babrock KL (1976) Predicting specific conductance and salt concentration in dilute aqueous solutions. Soil Sci 122:181–187

    Article  CAS  Google Scholar 

  • Nielsen KM, van Elsas JD (2001) Stimulatory effects of compounds present in the rhizosphere on natural transformation of Acinetobacter sp. BD413 in soil. Soil Biol Biochem 33:345–357

    Article  CAS  Google Scholar 

  • Nielsen KM, Galamai L, Pietramellara G (2006) Stabilization of extracellular DNA and proteins by transient binding to various soil compounds. In: Nannipieri P, Smalla K (eds) Soil biology, vol. 8. Nucleic acids and proteins in soil. Springer, Heidelberg, Germany, pp 141–158

    Chapter  Google Scholar 

  • Oger P, Petit A, Dessaux Y (1997) Genetically engineered plants producing opines alter their biological environment. Nat Biotech 15:369–372

    Article  CAS  Google Scholar 

  • Ogram AV, Sayler GS, Gustin D, Lewis JR (1988) DNA adsorption to soils and sediments. Environ Sci Technol 60:393–396

    Google Scholar 

  • Ogram AV, Mathot ML, Harsh BJ, Boyle J, Pettigrew CA Jr (1994) Effects of DNA polymer length onits adsorption to soils. Appl Environ Microbiol 60:393–396

    CAS  PubMed  Google Scholar 

  • Ohashi F, Wada S-I, Suzuki M, Maeda M, Tomura S (2002) Synthetic allophone from high concentration solutions: nanoengineering of the porous solid. Clay Minerals 37:451–456

    Article  CAS  Google Scholar 

  • Paget E, Monrozier LJ, Simonet P (1992) Adsorption of DNA on clay minerals: protection against DNaseI and influence on gene transfer. FEMS Microbiol Let 97:31–40

    Article  CAS  Google Scholar 

  • Pietramellara G, Ascher J, Ceccherini MT, Nannipieri P, Wenderoth D (2007) Adsorption of pure and dirty bacterial DNA on clay minerals and their transformation frequency. Biol Fertil Soils 43:731–739

    Article  CAS  Google Scholar 

  • Pietramellara G, Ascher J, Borgogni F, Ceccherini MT, Guerri G, Nannipieri P (2009) Extracellular DNA in soil and sediment: fate and ecological relevance. Biol Fertil Soils 45:219–235

    Article  CAS  Google Scholar 

  • Romanowski G, Lorenz MG, Wackernagel W (1991) Adsorption of plasmid DNA to mineral surfaces and protection against DNase I. Appl Environ Microbiol 57:1057–1061

    CAS  PubMed  Google Scholar 

  • Saeki K (2008) The comparison of arsenic adsorptions on an andosol between arsenite and arsenate. Soil Sci 173:248–256

    Article  CAS  Google Scholar 

  • Saeki K, Matsumoto S (1994) Selenite adsorption by a variety of oxide. Commun Soil Sci Plant Anal 25:2147–2158

    Article  CAS  Google Scholar 

  • Saeki K, Sakai M (2009) The influence of soil organic matter on DNA adsorptions on andosols. Microbes Environ 24:175–179

    Article  Google Scholar 

  • Saeki K, Morisaki M, Sakai M (2008) The effects of hydrogen peroxide and acid oxalate treatments on DNA adsorption on soils. Microbes Environ 23:353–355

    Article  Google Scholar 

  • Stotzky G (2000) Persistence and biological activity in soil of insecticidal proteins from Bacillus thuringiensis and of bacterial DNA bound on clays and humic acids. J Environ Qual 29:691–705

    Article  CAS  Google Scholar 

  • Takada-Hoshino Y, Matsumoto N (2004) An improved DNA extraction method using skim milk from soils that strongly absorb DNA. Microbes Environ 19:13–19

    Article  Google Scholar 

  • Theng BKG, Russell M, Churchman GJ, Parfitt RL (1982) Surface properties of allophanes, halloysite and imogolite. Clays Clay Miner 30:143–149

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors thank Mrs. T.Y.Kim for help in the experiments. This research was supported by a grant-in-aid for scientific research (B) from the Japan Society for the Promotion of Science (19380044).

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Authors and Affiliations

  1. Biotron Institute, Kyushu University, Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan

    Kazutoshi Saeki

  2. Faculty of Science, Shinshu University, Asahi, Matsumoto, 390-8621, Japan

    Takashi Kunito

  3. Faculty of Agriculture, Kagoshima University, Korimoto, Kagoshima, 890-0065, Japan

    Masao Sakai

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  1. Kazutoshi Saeki
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  2. Takashi Kunito
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  3. Masao Sakai
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Correspondence to Kazutoshi Saeki.

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Saeki, K., Kunito, T. & Sakai, M. Effects of pH, ionic strength, and solutes on DNA adsorption by andosols. Biol Fertil Soils 46, 531–535 (2010). https://doi.org/10.1007/s00374-010-0447-y

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  • DOI: https://doi.org/10.1007/s00374-010-0447-y

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