Abstract
Various biologically mediated processes are involved in the turnover of dissolved organic matter (DOM) in soil; however, relatively little is known about the dynamics of either the microbial community or the individual classes of organic molecules during the decomposition of DOM. We examined the net loss of DOC, the mineralisation of C to CO2 and the degradation of DOC from six different soils by soil microorganisms. We also quantified the changes in the concentrations of protein, carbohydrate and amino acid C during microbial biodegradation. Over a 70-day incubation period at 20°C, the mineralisation of DOC to CO2 was described by a double exponential model with a labile pool (half-life, 3–8 days) and a stable pool (half-life, 0.4–6 years). However, in nearly all cases, the mass loss of DOC exceeded the C released as CO2 with significant deviations from the double exponential model. Comparison of mass DOC loss, CO2 production and microbial cell counts, determined by epifluorescence microscopy, showed that a proportion of the lost DOC mass could be accounted for by microbial assimilation. Carbohydrate and protein C concentrations fluctuated throughout the incubation with a net change of between 3 to 13 and −30 to 22.4% initial DOC, respectively. No amino acid C was detected during the incubation period (level of detection, 0.01 mg C l−1).
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References
Allen SE (1989) Chemical analysis of ecological materials. Blackwell Scientific, UK
Boyer JN, Groffman PM (1996) Bioavailability of water extractable organic carbon fractions in forest and agricultural soil profiles. Soil Biol Biochem 28:783–790
Brooks PD, McKnight DM, Bencala KE (1999) The relationship between soil heterotrophic activity, soil dissolved organic carbon (DOC) leachate, and catchment-scale DOC export in headwater catchments. Water Resour Res 35:1895–1902
Damman AHW, French TW (1987) The ecology of peat bogs of the glaciated Northeastern United States. U.S. Fish and Wildlife Service Biological Report 85 (7.16). Supt of Documents, Washington, DC
Davey ME, O'Toole GA (2000) Microbial biofilms: from ecology to molecular genetics. Microb Mol Biol Rev 64:847–867
Davidson EA, Galloway LF, Strand MK (1987) Assessing available carbon: comparison of techniques across selected forest soils. Commun Soil Sci Plant Anal 18:45–64
Freeman C, Evans CD, Monteith DT (2001) Export of organic carbon from peat soils. Nat 412:785
Gregorich EG, Beare MH, Stoklas U, St-Georges P (2003) Biodegradability of soluble organic matter in maize-cropped soils. Geoderma 113:237–252
Guggenberger G, Kaiser K (2003) Dissolved organic matter in soil: challenging the paradigm of sorptive preservation. Geoderma 113:293–310
Guggenberger G, Zech W, Schulten H-R (1994) Formation and mobilisation pathways of dissolved organic matter: evidence from chemical structural studies of organic matter fractions in acid forest floor solutions. Org Geochem 21:51–66
Herbert BE, Bertsch PM (1995) Characterisation of dissolved and colloidal organic matter in soil solutions: a review. In: Kelley JM, McFee WW (eds) Carbon forms and functions in forest soils. Soil Science Society America, Madison, WI, pp 63–88
Hobbie JE, Daley RJ, Jasper S (1977) Use of Nucleopre filters for counting bacteria by fluorescence microscopy. Appl Environ Microbiol 33:1225–1228
Hongve D, Van Hees PAW, Lundstrom US (2000) Dissolved components in precipitation water percolated through forest litter. Eur J Soil Sci 51:667–677
Huang Y, Eglinton G, VanDer Hage ERE, Boon JJ, Bol R (1998) Dissolved organic matter and its parent organic matter in grass upland soil horizons studied by analytical pyrolysis techniques. Eur J Soil Sci 49:1–15
Jardine PM, Weber NL, McCarthy JF (1989) Mechanisms of dissolved organic carbon adsorption on soil. Soil Sci Soc Am J 53:1378–1385
Kaiser K, Guggenberger G, Haumaier L, Zech W (2001) Seasonal variations in the chemical composition of dissolved organic matter in organic forest floor layer leachates of old-growth Scots pine (Pinus sylvestris L.) and European beech (Fagus sylvatica L.) stands in northeastern Bavaria, Germa. Biogeochemistry 55:103–143
Kalbitz K, Popp P (1999) Seasonal impacts on β-hexachlorocyclohexane concentration in soil solution. Environ Pollut 106:139–141
Kalbitz K, Solinger S, Park J-H, Michalzik B, Matzner E (2000) Controls on the dynamics of dissolved organic carbon in soils: a review. Soil Sci 165:277–304
Kalbitz K, Schmerwitz J, Schwesig D, Matzner E (2003) Biodegradation of soil-derived dissolved organic matter as related to its properties. Geoderma 113:273–291
Kessler T, Harvey CF (2001) The global flux of CO2 into groundwater. Geophys Res Let 28:279–282
Kielland K (1994) Amino acid uptake by arctic plants. Ecology 75:2373–2383
Koivula N, Hänninen K (2001) Concentrations of monosaccharides in humic substances in the early stages of humification. Chemosphere 44:271–279
Küsel K, Drake HL (1999) Microbial trunover of low molecular weight organic acids during leaf litter decomposition. Soil Biol Biochem 31:107–118
Ladd JN, Jocteur-Monrozier L, Amato M (1992) Carbon turnover and nitrogen transformations in an alfisol and vertisol amended with [U-14C] glucose and [15 N]ammonium sulphate. Soil Biol Biochem 24:359–371
Lundström US, Van Breeman N, Jongmans AG (1995) Evidence for microbial decomposition of organic acids during podzolization. Eur J Soil Sci 46:489–496
Marschner B (1998) DOM-enhanced mobilization of benzo(a) pyrene in a contaminated soil under different chemical conditions. Phys Chem Earth 23:199–203
McDowell W, Likens GE (1988) Origin, composition, and flux of dissolved organic carbon in the Hubbard Brook Valley. Ecol Monogr 58:177–196
McDowell WH (2003) Dissolved organic matter in soils—future directions and unanswered questions. Geoderma 113:179–186
Meitinnen KJ (1959) Assimilation of amino acids in higher plants. Society of Experimental Biology symposium 13. Academy, New York
Meli SM, Badalucco L, English LC, Hopkins DW (2003) Respiratory responses of soil micro-organisms to simple and complex organic substrates. Biol Fertil Soils 37:96–101
Moore S, Stein WH (1951) Aminoacid determination, methods and techniques. J Biol Chem 192:663–670
Näsholm T, Ekblad A, Nordin A, Giesler R, Högberg M, Högberg P (1998) Boreal forest plants take up organic nitrogen. Nature 392:914–916
Nelson PN, Dictor M-C, Soulas G (1994) Availability of organic carbon in soluble and particle-size fractions from a soil profile. Soil Biol Biochem 26:1549–1555
Ohnishi ST, Barr JK (1978) A simplified method of quantitating proteins using the biuret and phenol reagents. Anal Biochem 86:193
Paul EA, Clark FE (1996) Soil microbiology and biochemistry. Academic, USA
Plummer LN, Busenberg B (1982) The solubilities of calcite, aragonite and vaterite in CO2–H2O solutions between 0 and 90 C, and an evaluation of the aqeuous model for the system CaCO3–CO2–H2O. Geochim Cosmochim Acta 46:1011–1040
Qualls RG (2000) Comparison of the behaviour of soluble organic and inorganic nutrients in forest soils. For Ecol Manag 138:29–50
Qualls RG, Haines BL (1991) Geochemistry of dissolved organic nutrients in water percolating through a forest ecosystem. Soil Sci Soc Am J 55:1112–1123
Qualls RG, Haines BL (1992) Biodegradability of dissolved organic matter in forest throughfall, soil solution and stream water. Soil Sci Soc Am J 56:578–586
Stevenson FJ (1994) Humus chemistry. Wiley, New York
Tate RL (1987) Soil organic matter: biological and ecological effects. Wiley, New York
WHO (1998) Guidelines for drinking water quality. Addendum to volume 1: Recommendations
Zech W, Guggenberger G (1996) Amount, fate, and function of dissolved organic matter in temperate forest ecosystems. In: Piccolo A (ed) Humic substances in terrestrial ecosystems. Elsevier Science, Amsterdam, Netherlands, pp 139–170
Zsolnay A (1996) Dissolved humus in soil waters. In: Piccolo A (ed) Humic substances in terrestrial ecosystems. Elsevier Science, Amsterdam, Netherlands, pp 171–223
Zsolnay A (2003) Dissolved organic matter: artefacts, definitions and functions. Geoderma 113:187–209
Zsolnay A, Steindl H (1991) Geovariability and biodegradability of the water-extractable organic material in an agricultural soil. Soil Biol Biochem 23:1077–1082
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We are grateful to the University of Stirling and the UK Biotechnology and Biological Sciences Research Council for supporting this work.
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Bowen, S.R., Gregorich, E.G. & Hopkins, D.W. Biochemical properties and biodegradation of dissolved organic matter from soils. Biol Fertil Soils 45, 733–742 (2009). https://doi.org/10.1007/s00374-009-0387-6
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DOI: https://doi.org/10.1007/s00374-009-0387-6