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Contributions of nitrification and denitrification to N2O emissions from soils at different water-filled pore space

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Abstract

A combination of stable isotope and acetylene (0.01% v/v) inhibition techniques were used for the first time to determine N2O production during denitrification, autotrophic nitrification and heterotrophic nitrification in a fertilised (200 kg N ha−1) silt loam soil at contrasting (20–70%) water-filled pore space (WFPS). 15N-N2O emissions from 14NH415NO3 replicates were attributed to denitrification and 15N-N2O from 15NH415NO3 minus that from 14NH415NO3 replicates was attributed to nitrification and heterotrophic nitrification in the presence of acetylene, as there was no dissimilatory nitrate reduction to ammonium or immobilisation and remineralisation of 15N-NO3−. All of the N2O emitted at 70% WFPS (31.6 mg N2O-N m−2 over 24 days; 1.12 μg N2O-N g dry soil−1; 0.16% of N applied) was produced during denitrification, but at 35–60% WFPS nitrification was the main process producing N2O, accounting for 81% of 15N-N2O emitted at 60% WFPS, and 7.9 μg 15N-N2O m−2 (0.28 ng 15N-N2O g dry soil−1) was estimated to be emitted over 7 days during heterotrophic nitrification in the 50% WFPS treatment and accounted for 20% of 15N-N2O from this treatment. Denitrification was the predominant N2O-producing process at 20% WFPS (2.6 μg 15N-N2O m−2 over 7 days; 0.09 ng 15N-N2O g dry soil−1; 85% of 15N-N2O from this treatment) and may have been due to the occurrence of aerobic denitrification at this WFPS. Our results demonstrate the usefulness of a combined stable isotope and acetylene approach to quantify N2O emissions from different processes and to show that several processes may contribute to N2O emission from agricultural soils depending on soil WFPS.

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Acknowledgements

This work was funded by a Research Committee studentship awarded by the Biotechnology and Biological Sciences Research Council, UK. We thank Jon Fear for the stable isotope analyses and Trudi Krol for assisting with the soil mineral N analyses.

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  1. E. J. Bateman

    Present address: Department of Biology 3, University of York, P.O. Box 373, York, YO10 5YW, UK

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  1. Department of Agricultural Sciences, Wye Campus, Imperial College London, Wye, Ashford, Kent, TN25 5AH, UK

    E. J. Bateman

  2. School of Biological Sciences, University of Aberdeen, Cruickshank Building, St. Machar Drive, Aberdeen, AB24 3UU, UK

    E. M. Baggs

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Correspondence to E. M. Baggs.

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Bateman, E.J., Baggs, E.M. Contributions of nitrification and denitrification to N2O emissions from soils at different water-filled pore space. Biol Fertil Soils 41, 379–388 (2005). https://doi.org/10.1007/s00374-005-0858-3

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