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Molecular and conventional responses of large rainbow trout to dietary phosphorus restriction

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Abstract

The dietary phosphorus (P) requirement for large fish is difficult to estimate because of insensitivities of known P status indicators. We examined dietary P requirement of large rainbow trout (mean body weight 278 g) using recently identified P-responsive genes (mRNA abundances) as well as conventional serum P and bone P. Fish were fed six diets (varied P contents), and the tissues of intestine, pyloric caeca (PC), kidney, serum and bone were collected at varying time intervals. Serum P responded clearly to dietary P by day 2, but the estimated P requirement based on this variable changed as feeding duration continued. Bone P did not respond clearly until week 7. Among P-responsive genes studied, Na/Pi cotransporter in PC (PC-NaPi) was the most sensitive, and responded in 2 days. Fish-to-fish (within treatment) variance was larger in mRNA than in serum P and bone P levels. Estimated dietary P requirements (%P in dry diet) were 0.45 (based on serum P), 0.45 (based on bone P), 0.36 (based on PC-NaPi), 0.33 (based on intestinal NaPi), 0.71 (based on renal NaPi), and 0.33 (based on mitochondrial Pi carrier). This study is the first to evaluate the potential of genomic approaches in determining nutrient requirements of fish.

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Abbreviations

HPRT:

Hypoxanthine phosphoribosyl transferase

I-NaPi:

Intestinal NaPi

K-NaPi:

Renal NaPi

mtPi:

Mitochondrial Pi carrier

NaPi:

Na/Pi cotransporter

P:

Phosphorus

PC:

Pyloric caeca

PC-NaPi:

Pyloric caecal NaPi

Pi:

Inorganic phosphorus

PiUS:

Pi uptake stimulator

QPCR:

Quantitative real-time PCR

S100CaBP:

S100 calcium binding protein

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Acknowledgments

We would like to thank the staff of the Ed Weed Fish Culture Station, Vermont Department of Fish & Wildlife for daily fish care in feeding trial. We also thank Dr. S. Kirchner for her help in tissue sampling. This study was supported by US Department of Agriculture/National Research Initiative (grant 2004-35206-14154 and 2003-35102-13520) and National Science Foundation (grant IBN-0235011). This study complied with the guidelines of the Institutional Animal Care & Use Committee (IACUC), University of Medicine & Dentistry of New Jersey (protocol #02038).

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Authors and Affiliations

  1. Department of Pharmacology & Physiology, New Jersey Medical School, University of Medicine & Dentistry of New Jersey, Newark, NJ, 07103, USA

    Shozo H. Sugiura & Ronaldo P. Ferraris

  2. Ed Weed Fish Culture Station, Vermont Department of Fish & Wildlife, Grand Isle, VT, 05458, USA

    Kevin Kelsey

  3. School of Environmental Science, University of Shiga Prefecture, 2500 Hassaka, Hikone, 522-8533, Japan

    Shozo H. Sugiura

Authors
  1. Shozo H. Sugiura
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  2. Kevin Kelsey
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  3. Ronaldo P. Ferraris
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Correspondence to Shozo H. Sugiura.

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Communicated by H.V. Carey.

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Sugiura, S.H., Kelsey, K. & Ferraris, R.P. Molecular and conventional responses of large rainbow trout to dietary phosphorus restriction. J Comp Physiol B 177, 461–472 (2007). https://doi.org/10.1007/s00360-007-0144-9

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  • DOI: https://doi.org/10.1007/s00360-007-0144-9

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