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Temperature-dependent growth rates and gene expression patterns of various medaka Oryzias latipes cell lines derived from different populations

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Abstract

Medaka Oryzias latipes has several geographically and genetically distinct populations. We examined temperature acclimation response in various medaka cell lines derived from different populations. Measurement of cell growth at various temperatures suggested that 15°C was the permissive growth temperature in all cell lines from the Northern Japanese and East Korean populations, but not in those from the Southern Japanese population and medaka-related species Oryzias celebensis, which inhabits a tropical zone. RT-PCR for 102 temperature-responsive genes, previously reported in other species, revealed that the accumulated mRNA level of a gene encoding HSP47 was lower at 25°C than at 33°C, and vice versa for 12 genes including IκBα and Rab-1c, in OLHNI-1 cell line from the Northern Japanese population. Further analysis by real-time PCR demonstrated that the accumulated mRNA levels of IκBα and Rab-1c in OLHNI-1 and OLSOK-e7 cell lines from the East Korean population were increased when the culture temperature was shifted from 33 to 15°C, but not in OLHdrR-e3 cell line from the Southern Japanese population. Since IκBα and Rab-1c are related to the NFκB cascade and endoplasmic reticulum-to-Golgi transport, respectively, it is inferred that immune responses and intracellular transport are possibly critical to temperature adaptation for medaka.

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Abbreviations

ALAD:

δ-Aminolevulinic acid dehydratase

COP9:

Constitutive photomorphogenic 9

EF-1α:

Elongation factor-1α

EST:

Expressed sequence tag

HSP:

Heat shock protein

IκB:

Inhibitor of NFκB

LDHB:

Lactate dehydrogenase B

ND1:

NADH dehydrogenase subunit 1

NFκB:

Nuclear factor-κB

PABPC1:

Poly(A) binding protein, cytoplasmic 1

PBS:

Phosphate buffered saline

RT-PCR:

Reverse transcription-polymerase chain reaction

SDHD:

Succinate dehydrogenase complex subunit D

TIM10:

Translocase of inner mitochondrial membrane 10

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Acknowledgements

We would like to express our sincere thanks to Dr. M. N. Ahsan, University of Khulna, Bangladesh, for critical reading of the manuscript. Thanks are also due to T. Kobayashi, The University of Tokyo, for preparation of medaka cultured cell lines. This work was funded in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan. M.H. was supported by Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists.

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  1. Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 113-8657, Bunkyo, Tokyo, Japan

    Makoto Hirayama & Shugo Watabe

  2. Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 277-8561, Kashiwa, Chiba, Japan

    Hiroshi Mitani

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  1. Makoto Hirayama
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Correspondence to Shugo Watabe.

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Hirayama, M., Mitani, H. & Watabe, S. Temperature-dependent growth rates and gene expression patterns of various medaka Oryzias latipes cell lines derived from different populations. J Comp Physiol B 176, 311–320 (2006). https://doi.org/10.1007/s00360-005-0053-8

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