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Color-coded three-dimensional micro particle tracking velocimetry and application to micro backward-facing step flows

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Abstract

In this work, the authors proposed a microscopic particle tracking system based on the previous work (Tien et al. in Exp Fluids 44(6):1015–1026, 2008). A three-pinhole plate, color-coded by color filters of different wavelengths, is utilized to create a triple exposure pattern on the image sensor plane for each particle, and each color channel of the color camera acts as an independent image sensor. This modification increases the particle image density of the original monochrome system by three times and eliminates the ambiguities caused by overlap of the triangle exposure patterns. A novel lighting method and a color separation algorithm are proposed to overcome the measurement errors due to crosstalk between color filters. A complete post-processing procedure, including a cascade correlation peak-finding algorithm to resolve overlap particles, a calibration-based method to calculate the depth location based on epipolar line search method, and a vision-based particle tracking algorithm is developed to identify, locate and track the Lagrangian motions of the tracer particles and reconstruct the flow field. A 10X infinity-corrected microscope and back-lighted by three individual high power color LEDs aligning to each of the pinhole is used to image the flow. The volume of imaging is 600 × 600 × 600 μm3. The experimental uncertainties of the system verified with experiments show that the location uncertainties are less than 0.10 and 0.08 μm for the in-plane and less than 0.82 μm for the out-of-plane components, respectively. The displacement uncertainties are 0.62 and 0.63 μm for the in-plane and 0.77 μm for the out-of-plane components, respectively. This technique is applied to measure a flow over a backward-facing micro-channel flow. The channel/step height is 600/250 μm. A steady flow with low particle density and an accelerating flow with high particle density are measured and compared to validate the flow field resolved from a two-frame tracking method. The Reynolds number in the current work varies from 0.033 to 0.825. A total of 20,592 vectors are reconstructed by time-averaged tracking of 156 image pairs from the steady flow case, and roughly 400 vectors per image pair are reconstructed by two-frame tracking from the accelerating flow case.

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References

  • Adrian RJ, Westerweel J (2011) Particle image velocimetry. Cambridge University Press, Cambridge

    Google Scholar 

  • Angarita-Jaimes NC, McGhee E, Chennaoui M, Campbell HI, Zhang S, Towers CE, Greenaway AH, Towers DP (2006) Wavefront sensing for single view three-component three-dimensional flow velocimetry. Exp Fluids 41(6):881–891

    Article  Google Scholar 

  • Angarita-Jaimes NC, Roca MAG, Towers CE, Read ND, Towers DP (2009) Algorithms for the automated analysis of cellular dynamics within living fungal colonies. Cytom Part A 75A(9):768–780

    Article  Google Scholar 

  • Bown MR, MacInnes JM, Allen RWK, Zimmerman WBJ (2006) Three-dimensional, three-component velocity measurements using stereoscopic micro-PIV and PTV. Meas Sci Technol 17(8):2175–2185

    Article  Google Scholar 

  • Chen S, Angarita-Jaimes N, Angarita-Jaimes D, Pelc B, Greenaway AH, Towers CE, Lin D, Towers DP (2009) Wavefront sensing for three-component three-dimensional flow velocimetry in microfluidics. Exp Fluids 47(4–5):849–863

    Article  Google Scholar 

  • Chirokov A (2012) Scattered data interpolation and approximation using radial base functions. http://www.mathworks.com/matlabcentral/fileexchange/10056-scattered-data-interpolation-and-approximation-using-radial-base-functions

  • Cierpka C, Segura R, Hain R, Kaehler CJ (2010) A simple single camera 3C3D velocity measurement technique without errors due to depth of correlation and spatial averaging for microfluidics. Meas Sci Technol 21(4):045401

    Article  Google Scholar 

  • Cierpka C, Rossi M, Segura R, Kaehler CJ (2011) On the calibration of astigmatism particle tracking velocimetry for microflows. Meas Sci Technol 22(1):015401

    Article  Google Scholar 

  • Duncan J, Dabiri D, Hove J, Gharib M (2010) Universal outlier detection for particle image velocimetry (PIV) and particle tracking velocimetry (PTV) data. Meas Sci Technol 21(5):057002

    Article  Google Scholar 

  • Grothe R, Rixon G, Dabiri D (2008) An improved three-dimensional characterization of defocusing digital particle image velocimetry (DDPIV) based on a new imaging volume definition. Meas Sci Technol 19:065402

    Article  Google Scholar 

  • Kajitani L, Dabiri D (2005) A full three-dimensional characterization of defocusing digital particle image velocimetry. Meas Sci Technol 16(3):790–804

    Article  Google Scholar 

  • Lei Y, Tien W, Duncan J, Paul M, Dabiri D, Rösgen T, Hove J (2012) A vision-based hybrid particle tracking velocimetry (PTV) technique using a modified cascade-correlation peak-finding method. Exp Fluids 53(5):1251–1268

    Article  Google Scholar 

  • Lindken R, Westerweel J, Wieneke B (2006) Stereoscopic micro particle image velocimetry. Exp Fluids 41(2):161–171

    Article  Google Scholar 

  • Luo R, Sun Y (2011) Pattern matching for three-dimensional tracking of sub-micron fluorescent particles. Meas Sci Technol 22(4):045402

    Article  MathSciNet  Google Scholar 

  • Luo R, Yang XY, Peng XF, Sun YF (2006) Three-dimensional tracking of fluorescent particles applied to micro-fluidic measurements. J Micromech Microeng 16(8):1689–1699

    Article  Google Scholar 

  • Maas HG, Gruen A, Papantoniou D (1993) Particle tracking velocimetry in 3-dimensional flows. 1. Photogrammetric determination of particle coordinates. Exp Fluids 15(2):133–146

    Article  Google Scholar 

  • Ooms TA, Lindken R, Westerweel J (2009) Digital holographic microscopy applied to measurement of a flow in a T-shaped micromixer. Exp Fluids 47(6):941–955

    Article  Google Scholar 

  • Park JS, Kihm KD (2006) Three-dimensional micro-PTV using deconvolution microscopy. Exp Fluids 40(3):491–499

    Article  Google Scholar 

  • Pereira F, Gharib M (2002) Defocusing digital particle image velocimetry and the three-dimensional characterization of two-phase flows. Meas Sci Technol 13(5):683–694

    Article  Google Scholar 

  • Pereira F, Gharib M, Dabiri D, Modarress D (2000) Defocusing digital particle image velocimetry: a 3-component 3-dimensional DPIV measurement technique. Application to bubbly flows. Exp Fluids 29:S78–S84

    Article  Google Scholar 

  • Pereira F, Lu J, Castano-Graff E, Gharib M (2007) Microscale 3D flow mapping with mu DDPIV. Exp Fluids 42(4):589–599

    Article  Google Scholar 

  • Peterson SD, Chuang H, Wereley ST (2008) Three-dimensional particle tracking using micro-particle image velocimetry hardware. Meas Sci Technol 19(11):115406

    Article  Google Scholar 

  • Richards JA, Jia X (1999) Remote sensing digital image analysis: an introduction. Springer, Berlin

    Book  Google Scholar 

  • Santiago J, Wereley S, Meinhart C, Beebe D, Adrian R (1998) A particle image velocimetry system for microfluidics. Exp Fluids 25(4):316–319

    Article  Google Scholar 

  • Satake S, Kunugi T, Sato K, Ito T, Kanamori H, Taniguchi J (2006) Measurements of 3D flow in a micro-pipe via micro digital holographic particle tracking velocimetry. Meas Sci Technol 17(7):1647–1651

    Article  Google Scholar 

  • Scott GL, Longuet-higgins HC (1991) An algorithm for associating the features of 2 images. Proc R Soc Lond Ser B Biol Sci 244(1309):21–26

    Article  Google Scholar 

  • Sheng J, Malkiel E, Katz J (2006) Digital holographic microscope for measuring three-dimensional particle distributions and motions. Appl Opt 45(16):3893–3901

    Article  Google Scholar 

  • Tien W-H, Kartes P, Yamasaki T, Dabiri D (2008) A color-coded backlighted defocusing digital particle image velocimetry system. Exp Fluids 44(6):1015–1026

    Article  Google Scholar 

  • Towers CE, Towers DP, Campbell HI, Zhang SJ, Greenaway AH (2006) Three-dimensional particle imaging by wavefront sensing. Opt Lett 31(9):1220–1222

    Article  Google Scholar 

  • Willert CE, Gharib M (1992) Three-dimensional particle imaging with a single camera. Exp Fluids 12:353–358

    Article  Google Scholar 

  • Yoon SY, Kim KC (2006) 3D particle position and 3D velocity field measurement in a microvolume via the defocusing concept. Meas Sci Technol 17(11):2897–2905

    Article  Google Scholar 

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Acknowledgments

The authors gratefully acknowledge the support of the National Institutes of Health (R01 RR023190-04) and the Murdock Trust Foundation.

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Correspondence to Dana Dabiri.

Additional information

This article is part of the Topical Collection on Application of Laser Techniques to Fluid Mechanics 2012.

Appendix

Appendix

The radial basis function (RBF) interpolation is a method for approaching a function with given data points. The RBF is a real-valued function depending only on the distance from a certain point,

$$ \varphi \left( r \right) = \varphi \left( {\left|| {x - x_{i} } \right||} \right), $$
(15)

where \( r = \left( {\left|| {x - x_{i} } \right||} \right) \) is the Euclidean distance in the current work.

For N given points, the target function can be approximated by the sum of N radial basis function,

$$ f\left( x \right) = c_{0} + c_{1} x + \mathop \sum \limits_{i = 1}^{N} \lambda_{i} \varphi \left( {\left| {x - x_{i} } \right|} \right), $$
(16)

where the coefficients c 0 , c 1, and λ i are chosen to match the function values at the known data points (interpolation nodes). Because the approximation function is linear to the coefficients, these coefficients can be estimated using matrix methods of linear least squares. Several basis functions can be used are listed below:

Gaussian:

$$ \varphi \left( r \right) = \exp \left( { - \frac{{r^{2} }}{{2\sigma^{2} }}} \right), $$
(17)

Multiquadrics:

$$ \varphi \left( r \right) = \exp \sqrt {\left( {1 + \frac{{r^{2} }}{{\sigma^{2} }}} \right)} , $$
(18)

Linear:

$$ \varphi \left( r \right) = r, $$
(19)

Cubic:

$$ \varphi \left( r \right) = r^{3} , $$
(20)

Thinplate:

$$ \varphi \left( r \right) = \ln (r + 1) $$
(21)

once coefficients c 0 , c 1,and λ i are found, this expression can be used to estimate value of the function at any point.

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Tien, WH., Dabiri, D. & Hove, J.R. Color-coded three-dimensional micro particle tracking velocimetry and application to micro backward-facing step flows. Exp Fluids 55, 1684 (2014). https://doi.org/10.1007/s00348-014-1684-x

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  • DOI: https://doi.org/10.1007/s00348-014-1684-x

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