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Adipose-derived stem cells (ADSCs) and muscle precursor cells (MPCs) for the treatment of bladder voiding dysfunction

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World Journal of Urology Aims and scope Submit manuscript

An Erratum to this article was published on 02 February 2014

Abstract

Purpose

Bladder outflow obstruction (BOO) is common in the elderly and can result in bladder voiding dysfunction (BVD) due to severe bladder muscle damage. The goal of this research was to evaluate the use of adult stem cells for the treatment of BVD due to decreased muscle contractility in a rat model.

Materials and methods

Adipose-derived stem cells (ADSCs) and muscle precursor cells (MPCs) were harvested from male Lewis rats and expanded in culture. BOO was induced by tying a suture around the urethra. Six weeks after obstruction, the development of BVD was confirmed by cystometric analysis in conscious rats, histology and molecular investigations. Injection of ADSCs or MPCs into the bladder wall and synchronous deligation was performed 6 weeks after the obstruction. After stem-cell treatment, morphological and functional changes were assessed. Age-matched rats and animals without cellular therapy but deligation-only served as controls.

Results

Voiding pressures decreased progressively 6 weeks after obstruction with increased bladder capacities. Structural changes of the detrusor muscle occurred during the time of obstruction with an increased connective tissue-to-smooth muscle ratio and decreased SMA/smoothelin expression. After stem-cell injection, improved voiding pressures and voiding volumes were observed together with recovered tissue architecture. RT-PCR and Western blotting showed an up-regulation of important contractile proteins.

Conclusions

We established a reliable model for BVD and demonstrated that ADSCs and MPCs can prevent pathophysiological remodelling and provide regenerated bladder tissue and function.

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Acknowledgments

We would like to thank Damina Balmer, M. Sc., for her assistance in the preparation of the manuscript. We are grateful to Irina Agarkova, PhD, for her support. This study was funded by Max und Hedwig Niedermaier-Stiftung and Fonds für Medizinische Forschung.

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Authors and Affiliations

  1. Division of Urology, University Hospital Zürich (USZ), 8091, Zurich, Switzerland

    Mathias Tremp, Souzan Salemi, Remo Largo, Tullio Sulser & Daniel Eberli

  2. Wake Forest University Health Sciences, Wake Forest Institute for Regenerative Medicine, Medical Center Boulevard, Winston Salem, NC, 27154-1094, USA

    Karl-Erik Andersson & Tamer Aboushwareb

  3. Division of Plastic and Reconstructive Surgery, Department of Surgery, University Hospital Zürich, 8091, Zurich, Switzerland

    Jan A. Plock

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  1. Mathias Tremp
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  2. Souzan Salemi
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Corresponding author

Correspondence to Daniel Eberli.

Additional information

Mathias Tremp and Souzan Salemi have contributed equally to this work.

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Tremp, M., Salemi, S., Largo, R. et al. Adipose-derived stem cells (ADSCs) and muscle precursor cells (MPCs) for the treatment of bladder voiding dysfunction. World J Urol 32, 1241–1248 (2014). https://doi.org/10.1007/s00345-013-1200-6

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  • DOI: https://doi.org/10.1007/s00345-013-1200-6

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