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Circulating microRNAs in serum: novel biomarkers for patients with bladder cancer?

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World Journal of Urology Aims and scope Submit manuscript

Abstract

Purpose

Recent studies indicate that circulating microRNAs in serum/plasma are a novel class of non-invasive biomarkers with diagnostic and prognostic information. So far, circulating microRNAs have not been analyzed in patients with bladder cancer.

Methods

We collected serum from patients with non-muscle invasive bladder cancer (NMIBC), muscle invasive bladder cancer (MIBC) and non-malignant urological disease. Total RNA was isolated from 400 μl of serum using the mirVana PARIS Kit; the artificial cel-miR-39 was spiked-in prior to RNA isolation to control different RNA isolation efficiencies. Quantitative real-time PCR was applied to measure the levels of 22 microRNAs upregulated in BCA tissue (miR-15a, miR-18a, miR-21, miR-93, miR-96, miR-103, miR-130b, miR-135b, miR-141, miR-182, miR-183, miR-190, miR-191, miR-200b, miR-422b, miR-425, miR-449b, miR-601, miR-639, miR-644, miR-649 and miR-1233) in the marker identification cohort (NMIBC, n = 11, MIBC, n = 10; controls, n = 10). The most promising serum microRNAs were tested in a validation cohort (NMIBC, n = 65, MIBC, n = 61; controls, n = 105).

Results

The RNA recovery was similar in patients with NMIBC, MIBC and control subjects. The analysis of serum microRNA levels in the marker identification cohort indicated that serum miR-141 and miR-639 levels were increased in bladder cancer patients compared to CTRL. The analysis of these miR-141 and miR-639 in the validation cohort demonstrated that microRNA levels were similar in bladder cancer patients and control subjects. Furthermore, microRNA levels were not correlated with clinicopathological parameters (pT-stage, metastasis, grading).

Conclusions

The analysis of serum miR-141 and miR-639 levels does not seem to be helpful in the diagnosis or prognosis of BCA.

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Acknowledgments

The study was supported by a research grant to Jörg Ellinger from the Deutsche Forschungsgemeinschaft (EL-623/1-1). The collection of serum samples was performed within the framework of the Biobank of the CIO Köln/Bonn.

Conflict of interest

The authors disclose any conflict of interest.

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Authors and Affiliations

  1. Klinik und Poliklinik für Urologie und Kinderurologie, Universitätsklinikum Bonn, Sigmund-Freud-Strasse 25, 53105, Bonn, Germany

    Anna-Regina Scheffer, Stefan C. Müller & Jörg Ellinger

  2. Institut für Klinische Chemie und Klinische Pharmakologie, Universitätsklinikum Bonn, Bonn, Germany

    Stefan Holdenrieder

  3. Institut für Pathologie, Universitätsklinikum Bonn, Bonn, Germany

    Glen Kristiansen & Alexander von Ruecker

Authors
  1. Anna-Regina Scheffer
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  2. Stefan Holdenrieder
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  3. Glen Kristiansen
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  4. Alexander von Ruecker
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  5. Stefan C. Müller
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  6. Jörg Ellinger
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Correspondence to Jörg Ellinger.

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Scheffer, AR., Holdenrieder, S., Kristiansen, G. et al. Circulating microRNAs in serum: novel biomarkers for patients with bladder cancer?. World J Urol 32, 353–358 (2014). https://doi.org/10.1007/s00345-012-1010-2

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  • DOI: https://doi.org/10.1007/s00345-012-1010-2

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