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Challenges of using mass spectrometry as a bladder cancer biomarker discovery platform

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Abstract

Introduction

Bladder cancer (BCa) is one of the most prevalent malignancies worldwide, mostly due to its high recurrence rates. In consequence, the necessity of repeated screening for reappearance demonstrates the urgent need for novel biomarkers as alternatives to invasive standard procedures.

Methods

Proteomic technologies have emerged as powerful platforms for unbiased biomarker discovery and revolutionized the classical “target-driven” analysis of single marker candidates. Although proteome profiling is still far from demonstrating its full potential in clinical diagnosis, first studies clearly denote its significant potential.

Conclusions

This review provides a discussion of the challenges related to clinical proteomics using mass spectrometry, emphasizing bladder cancer biomarker discovery. An outline of the technological prerequisites for reliable proteome profiling, data mining and interpretation, as well as, reflections on future trends in the field are provided.

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Acknowledgments

This work was partially funded by Eurotransbio grant ETB-2006-016 to the Urosysteomics consortium (http://www.urosysteomics.com) to E.S. and H.M.

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Authors and Affiliations

  1. Mosaiques Diagnostics and Therapeutics AG, Hannover, Germany

    Eric Schiffer & Harald Mischak

  2. University of Virginia Health Sciences Center, Charlottesville, VA, USA

    Dan Theodorescu

  3. Biotechnology Division, Biomedical Research Foundation, Academy of Athens, Soranou Efessiou 4, 11527, Athens, Greece

    Antonia Vlahou

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  1. Eric Schiffer
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  2. Harald Mischak
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  3. Dan Theodorescu
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  4. Antonia Vlahou
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Correspondence to Antonia Vlahou.

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Schiffer, E., Mischak, H., Theodorescu, D. et al. Challenges of using mass spectrometry as a bladder cancer biomarker discovery platform. World J Urol 26, 67–74 (2008). https://doi.org/10.1007/s00345-007-0234-z

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