Abstract
The light-emitting diodes (LEDs) are expected to be effective light sources for growing wheat in indoor cultivation due to their small size, long lifespan, high safety performance, and customized wavelengths. In the present study, the influences of LEDs light sources with different spectra combinations were investigated in wheat plants grown in hydroponic system. Eight types of different spectra were compared: white light (W), white–green light (W:G = 4:1, W4G1), red–green light (R:G = 4:1, R4G1), red–green–blue light (R:G:B = 4:1:1, R4G1B1), and four types of red–blue lights (R:B = 3:1, R3B1; R:B = 2:1, R2B1; R:B = 1:1, R1B1; R:B = 1:6, R1B6). The growth and development were monitored together with the activity of antioxidant enzymes in leaves. In addition, grain yield and quality were also investigated. The results showed that winter wheat could complete its life cycle under all eight LEDs light regimens in the completely enclosed hydroponic cultivation environment. Among them, plants in W, W4G1, and R4G1 grew fastest, followed by those of R4G1B1 and then by those of R3B1, R2B1, R1B1, and R1B6. Although blue light increased tillering number and dry matter production of whole plant, it was not conducive to distribution of dry matter to spikes and grain yield per plant. On the contrary, red light was positively correlated with dry matter distribution and accumulation in spikes. In addition, green light was helpful for the percentage of earbearing tiller, which constituted the number of spikes. Wheat grown in R4G1 had the higher spike number per plant, grain number per spike, and grain weight, leading to the highest grain yield per plant and harvest index. Meanwhile, the starch content, protein content, as well as the nutritional quality and processing quality of wheat flour grown under R4G1 could reach moderate levels. Taken together, appropriate LED’s light spectra, such as R4G1, are efficient for wheat cultivation in a controlled environment and make it possible to achieve faster, more, and better winter wheat production.
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Acevedo-Siaca LG, Coe R, Quick WP, Long SP (2021) Variation between rice accessions in photosynthetic induction in flag leaves and underlying mechanisms. J Exp Bot 72:1282–1294. https://doi.org/10.1093/jxb/eraa520
Akiyama T, Kozai T (2016) Light environment in the cultivation space of plant factory with LEDs. LED lighting for urban agriculture. Springer, Berlin, pp 91–109
Asseng S, Guarin JR, Raman M, Monje O, Kiss G, Despommier DD, Meggers FM, Gauthier PPG (2020) Wheat yield potential in controlled-environment vertical farms. Proc Natl Acad Sci USA 117:19131–19135. https://doi.org/10.1073/pnas.2002655117/-/DCSupplemental
Bartucca ML, Del Buono D, Ballerini E, Benincasa P, Falcinelli B, Guiducci M (2020) Effect of light spectrum on gas exchange, growth and biochemical characteristics of einkorn seedlings. Agronomy 10:1042. https://doi.org/10.3390/agronomy10071042
Blümel M, Dally N, Jung C (2015) Flowering time regulation in crops- what did we learn from Arabidopsis. Curr Opin Biotech 32:121–129. https://doi.org/10.1016/j.copbio.2014.11.023
Causin HF, Jauregui RN, Barneix AJ (2006) The effect of light spectral quality on leaf senescence and oxidative stress in wheat. Plant Sci 171:24–33. https://doi.org/10.1016/j.plantsci.2006.02.009
Chen M, Chory J, Fankhauser C (2004) Light signal transduction in higher plants. Annu Rev Genet 38:87–117. https://doi.org/10.1146/annurev.genet.38.072902.092259
Chen X, Yang Q, Song W, Wang L, Guo W, Xue X (2017) Growth and nutritional properties of lettuce affected by different alternating intervals of red and blue LED irradiation. Sci Hort 223:44–52. https://doi.org/10.1016/j.scienta.2017.04.037
Demotes-Mainard S, Péron T, Corot A, Bertheloot J, Le Gourrierec J, Pelleschi-Travier S, Crespel L, Morel P, Huché-Thélier L, Boumaza R, Vian A, Guérin V, Leduc N, Sakr S (2016) Plant responses to red and far-red lights, applications in horticulture. Environ Exp Bot 121:4–21. https://doi.org/10.1016/j.envexpbot.2015.05.010
Deng W, Clausen J, Boden S, Oliver SN, Casao MC, Ford B, Anderssen RS, Trevaskis B (2015) Dawn and dusk set states of the circadian osscillator in sprouting barley (Hordeum vulgare) seedlings. PLoS ONE 10:e129781. https://doi.org/10.1371/journal.pone.0129781
Dhindsa RS, Plumb-Dhindsa P, Thorpe TA (1981) Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. J Exp Bot 32:93–101. https://doi.org/10.1093/jxb/32.1.93
Dıaz J, Bernal A, Pomar F, Merino F (2001) Induction of shikimate dehydrogenase and peroxidase in pepper (Capsicum annuum L.) seedlings in response to copper stress and its relation to lignification. Plant Sci 161:179–188. https://doi.org/10.1016/S0168-9452(01)00410-1
Dong C, Fu Y, Liu G, Liu H (2014) Growth, photosynthetic characteristics, antioxidant capacity and biomass yield and quality of wheat (Triticum aestivum L.) exposed to led light sources with different spectra combinations. J Agron Crop Sci 200:219–230. https://doi.org/10.1111/jac.12059
Fan X, Xue F, Song B, Chen L, Xu G, Xu H (2019) Effects of blue and red light on growth and nitrate metabolism in Pakchoi. Open Chem 17:456–464. https://doi.org/10.1515/chem-2019-0038
Folta KM (2004) Green light stimulates early stem elongation, antagonizing light-mediated growth inhibition. Plant Physiol 135:1407–1416. https://doi.org/10.1104/pp.104.038893
Folta KM, Childers KS (2008) Light as a growth regulator: controlling plant biology with narrow-bandwidth solid-state lighting systems. Hortscience 43:1957–1964. https://doi.org/10.21273/HORTSCI.43.7.1957
Folta KM, Maruhnich SA (2007) Green light: a signal to slow down or stop. J Exp Bot 58:3099–3111. https://doi.org/10.1093/jxb/erm130
Folta KM, Pontin MA, Karlin-Neumann G, Bottini R, Spalding EP (2003) Genomic and physiological studies of early cryptochrome 1 action demonstrate roles for auxin and gibberellin in the control of hypocotyl growth by blue light. The Plant J 36:203–214. https://doi.org/10.1046/j.1365-313X.2003.01870.x
Gerdan PD, Chory J (2003) Regulation of flowering time by light quality. Nature 423:881–885. https://doi.org/10.1038/nature01636
Goins GD, Yorio NC, Sanwo MM, Brown CS (1997) Photomorphogenesis, photosynthesis, and seed yield of wheat plants grown under red light-emitting diodes (LEDs) with and without supplemental blue lighting. J Exp Bot 48:1407–1413. https://doi.org/10.1093/jxb/48.7.1407
Hanft JM, Jones RJ (1986) Kernel abortion in maize I. Carbohydrate concentration patterns and acid invertase activity of maize kernels induced to abort in vitro. Plant Physiol 81:503–510. https://doi.org/10.1104/pp.81.2.503
Hee-Sun Kook KK (2013) The Effect of blue-light-emitting diodes on antioxidant properties and resistance to botrytis cinerea in tomato. J Plant Pathol Microb 4:203. https://doi.org/10.4172/2157-7471.1000203
Heo JW, Lee CW, Paek KY (2006) Influence of mixed LED radiation on the growth of annual plants. J Plant Biol 49:286–290. https://doi.org/10.1007/BF03031157
Heo JW, Lee YB, Kim DE, Chang YS, Chun C (2010) Effects of supplementary led lighting on growth and biochemical parameters in Dieffenbachia Amoena ‘Camella’ and Ficus Elastica ‘Melany.’ Korean J Hortic Sci 28:51–58
Hoagland DR, Arnon DI (1950) The water-culture method for growing plants without soil. Cal Agric Exp St Circ 347:1–32
Huché-Thélier L, Crespel L, Gourrierec JL, Morel P, Sakr S, Leduc N (2016) Light signaling and plant responses to blue and UV radiations-Perspectives for applications in horticulture. Environ Exp Bot 121:22–38. https://doi.org/10.1016/j.envexpbot.2015.06.009
Huijser P, Schmid M (2011) The control of developmental phase transitions in plants. Development 138:4117–4129. https://doi.org/10.1242/dev.063511
Johkan M, Shoji K, Goto F, Hashida S, Yoshihara T (2010) Blue light-emitting diode light irradiation of seedlings improves seedling quality and growth after transplanting in red leaf lettuce. Hortscience 45:1809–1814. https://doi.org/10.21273/HORTSCI.45.12.1809
Kim H, Goins GD, Wheeler RM, Sager JC (2004) Green-light supplementation for enhanced lettuce growth under red- and blue-light-emitting diodes. Hortscience 39:1617–1622. https://doi.org/10.21273/HORTSCI.39.7.1617
Klein RM, Edsall PC, Gentile AC (1965) Effects of near ultraviolet and green radiations on plant growth. Plant Physio 40:903–906. https://doi.org/10.1104/pp.40.5.903
Koga R, Meng T, Nakamura E, Miura C, Irino N, Devkota HP, Yahara S, Kondo R (2013) The effect of photo-irradiation on the growth and ingredient composition of young green barley (Hordeum vulgare). Agric Sci 04:185–194. https://doi.org/10.4236/as.2013.44027
Li H, Tang C, Xu Z, Liu X, Han X (2012) Effects of different light sources on the growth of non-heading Chinese cabbage (Brassica campestris L.). J Agric Sci 4:262–273. https://doi.org/10.5539/jas.v4n4p262
Li Y, Xin G, Wei M, Shi Q, Yang F, Wang X (2017) Carbohydrate accumulation and sucrose metabolism responses in tomato seedling leaves when subjected to different light qualities. Sci Hort 225:490–497. https://doi.org/10.1016/j.scienta.2017.07.053
Li J, Xu X, Lin G, Wang Y, Liu Y, Zhang M, Zhou J, Wang Z, Zhang Y (2018) Micro-irrigation improves grain yield and resource use efficiency by co-locating the roots and N-fertilizer distribution of winter wheat in the North China Plain. Sci Total Environ 643:367–377. https://doi.org/10.1016/j.scitotenv.2018.06.157
Luo L, Hui X, Wang Z, Zhang X, Xie Y, Gao Z, Chai S, Lu Q, Li T, Sun M, Chang L, Bai Y, Malhi SS (2019) Multi-site evaluation of plastic film mulch and nitrogen fertilization for wheat grain yield, protein content and its components in semiarid areas of China. Field Crops Res 240:86–94. https://doi.org/10.1016/j.fcr.2019.06.002
Mahdavikia F, Saharkhiz MJ, Karami A (2017) Defensive response of radish seedlings to the oxidative stress arising from phenolic compounds in the extract of peppermint (Mentha× piperita L.). Sci Hort 214:133–140. https://doi.org/10.1016/j.scienta.2016.11.029
Manivannan A, Soundararajan P, Halimah N, Ko CH, Jeong BR (2015) Blue LED light enhances growth, phytochemical contents, and antioxidant enzyme activities of Rehmannia glutinosa cultured in vitro. Hort Environ Biotechnol 56:105–113. https://doi.org/10.1007/s13580-015-0114-1
McMaugh SJ, Thistleton JL, Anschaw E, Luo J, Konik-Rose C, Wang H, Huang M, Larroque O, Regina A, Jobling SA, Morell MK, Li Z (2014) Suppression of starch synthase I expression affects the granule morphology and granule size and fine structure of starch in wheat endosperm. J Exp Bot 65:2189–2201. https://doi.org/10.1093/jxb/eru095
Mittler R (2017) ROS are good. Trends Plant Sci 22:11–19. https://doi.org/10.1016/j.tplants.2016.08.002
Monostori I, Heilmann M, Kocsy G, Rakszegi M, Ahres M, Altenbach SB, Szalai G, Pál M, Toldi D, Simon-Sarkadi L, Harnos N, Galiba G, Darko É (2018) LED lighting-modification of growth, metabolism, yield and flour composition in wheat by spectral quality and intensity. Front Plant Sci 9:605. https://doi.org/10.3389/fpls.2018.00605
Pham MD, Hwang H, Park SW, Cui M, Lee H, Chun C (2019) Leaf chlorosis, epinasty, carbohydrate contents and growth of tomato show different responses to the red/blue wavelength ratio under continuous light. Plant Physiol Bioch 141:477–486. https://doi.org/10.1016/j.plaphy.2019.06.004
Reynolds M, Foulkes MJ, Slafer GA, Berry P, Parry MAJ, Snape JW, Angus WJ (2009) Raising yield potential in wheat. J Exp Bot 60:1899–1918. https://doi.org/10.1093/jxb/erp016
Reynolds M, Foulkes J, Furbank R, Griffiths S, King J, Murchie E, Parry M, Slafer G (2012) Achieving yield gains in wheat. Plant Cell Environ 35:1799–1823. https://doi.org/10.1111/j.1365-3040.2012.02588.x
Sairam RK, Rao KV, Srivastava GC (2002) Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Sci 163:1037–1046. https://doi.org/10.1016/S0168-9452(02)00278-9
Senger H (1982) The effect of blue-light on plants and microorganisms. Photochem Photobiol 35:911–920. https://doi.org/10.1111/j.1751-1097.1982.tb02668.x
Serrago RA, Miralles DJ, Slafer GA (2008) Floret fertility in wheat as affected by photoperiod during stem elongation and removal of spikelets at booting. Eur J Agron 28:301–308. https://doi.org/10.1016/j.eja.2007.08.004
Shen YZ, Guo SS (2014) Effects of photoperiod on wheat growth, development and yield in CELSS. Acta Astronaut 105:24–29. https://doi.org/10.1016/j.actaastro.2014.08.024
Shewry PR (2007) Improving the protein content and composition of cereal grain. J Cereal Sci 46:239–250. https://doi.org/10.1016/j.jcs.2007.06.006
Simlat M, Ślęzak P, Moś M, Warchoł M, Skrzypek E, Ptak A (2016) The effect of light quality on seed germination, seedling growth and selected biochemical properties of Stevia rebaudiana Bertoni. Sci Hortic 211:295–304. https://doi.org/10.1016/j.scienta.2016.09.009
Stewart RRC, Bewley JD (1980) Lipid peroxidation associated with accelerated aging of soybean axes. Plant Physiol 65:245–248. https://doi.org/10.1104/pp.65.2.245
Sysoeva MI, Markovskaya EF, Shibaeva TG (2010) Plants under continuous light: a review. Plant Stress 4:5–17
Tasleem-Tahir A, Nadaud I, Chambon C, Branlard G (2012) Expression profiling of starchy endosperm metabolic proteins at 21 stages of wheat grain development. J Proteome Res 11:2754–2773. https://doi.org/10.1021/pr201110d
Thomas B (2006) Light signals and flowering. J Exp Bot 57:3387–3393. https://doi.org/10.1093/jxb/erl071
Tripathi AK, Pareek A, Sopory SK, Singla-Pareek SL (2012) Narrowing down the targets for yield improvement in rice under normal and abiotic stress conditions via expression profiling of yield-related genes. Rice 5:1–12. https://doi.org/10.1186/1939-8433-5-37
Watson A, Ghosh S, Williams MJ, Cuddy WS, Simmonds J, Rey M, Asyraf MD, Hatta M, Hinchliffe A, Steed A, Reynolds D, Adamski NM, Breakspear A, Korolev A, Rayner T, Dixon LE, Riaz A, Martin W, Ryan M, Edwards D, Batley J, Raman H, Carter J, Rogers C, Domoney C, Moore G, Harwood W, Nicholson P, Dieters MJ, DeLacy IH, Zhou J, Uauy C, Boden SA, Park RF, Wulff BBH, Hickey LT (2018) Speed breeding is a powerful tool to accelerate crop research and breeding. Nat Plants 4:23–29. https://doi.org/10.1038/s41477-017-0083-8
Xu F, Shi L, Chen W, Cao S, Su X, Yang Z (2014) Effect of blue light treatment on fruit quality, antioxidant enzymes and radical-scavenging activity in strawberry fruit. Sci Hortic-Amsterdam 175:181–186. https://doi.org/10.1016/j.scienta.2014.06.012
Ye S, Shao Q, Xu M, Li S, Wu M, Tan X, Su L (2017) Effects of light quality on morphology, enzyme activities, and bioactive compound contents in Anoectochilus roxburghii. Front Plant Sci 8:857
Yeh N, Chung J (2009) High-brightness LEDs-Energy efficient lighting sources and their potential in indoor plant cultivation. Renew Sustain Energy Rev 13:2175–2180. https://doi.org/10.1016/j.rser.2009.01.027
Yu X, Chen X, Wang L, Yang Y, Zhu X, Shao S, Cui W, Xiong F (2017) Novel insights into the effect of nitrogen on storage protein biosynthesis and protein body development in wheat caryopsis. J Exp Bot 68:2259–2274. https://doi.org/10.1093/jxb/erx108
Zadoks JC, Chang TT, Konzak CF (1974) A decimal code for the growth stages of cereals. Weed Res 14:415–421. https://doi.org/10.1111/j.1365-3180.1974.tb01084.x
Zhang T, Shi Y, Piao F, Sun Z (2018) Effects of different LED sources on the growth and nitrogen metabolism of lettuce. Plant Cell Tissue Organ Cult 134:231–240. https://doi.org/10.1007/s11240-018-1415-8
Zhao J, Thi LT, Park YG, Jeong BR (2020) Light quality affects growth and physiology of Carpesium triste maxim. cultured in vitro. Agriculture 10:258. https://doi.org/10.3390/agriculture10070258
Acknowledgements
This work was funded by the National Natural Science Foundation of China (31871563), National & Local Joint Engineering Laboratory For Agricultural Internet of Things (2022), China Agriculture Research System of MOF and MARA (CARS-03), and Natural Science Foundation of Ningbo City (202003N4161). We gratefully acknowledge TIANDINGXING Optoelectronics Technology Co., Ltd., located in Ningbo, Zhejiang Province, China, for providing the LED lamps, and Yong Li, an engineer from the National Research Center of Intelligent Equipment for Agriculture, for his technical assistance on the precise control of the environment in control room.
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XLG performed the experiment, analyzed the data, wrote and revised the manuscript; XZX, YHZ, and ZMW conceived and designed the research; YHZ supervised the research; XZX, LLC, and JYL reviewed and edited the manuscript. All authors have read and agreed to the published version of the manuscript.
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Guo, X., Xue, X., Chen, L. et al. Effects of LEDs Light Spectra on the Growth, Yield, and Quality of Winter Wheat (Triticum aestivum L.) Cultured in Plant Factory. J Plant Growth Regul 42, 2530–2544 (2023). https://doi.org/10.1007/s00344-022-10724-z
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DOI: https://doi.org/10.1007/s00344-022-10724-z