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Amelioration of Morpho-structural and Physiological Disorders in Micropropagation of Aloe vera L. by Use of an Aromatic Cytokinin 6-(3-Hydroxybenzylamino) Purine

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Abstract

An in vitro regeneration method with functional morpho-structural and physiological traits has been optimized for Aloe vera L. in the present study. Among the various types and treatments of cytokinins [6-benzylaminopurine, BAP; 6-(3-hydroxybenzylamino) purine, mT (meta-topolin), and 1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea, TDZ] tested for shoot induction, the best bud break response (100%) occurred on the Murashige and Skoog (MS) medium containing 1.0 mg L−1 mT with the development of 5.0 shoots with 3.3 cm average length. Liquid MS medium containing a standardized combination of 0.5 mg L−1 mT with 0.25 mg L−1 α-naphthalene acetic acid (NAA) triggered proliferation of shoots, and maximum 42 shoots with 5.6 cm average length were obtained after 3rd subculture. Meta-topolin and NAA-derived shoots cultured on half strength MS medium containing 1.0 mg L−1 Indole-3-butyric acid (IBA) resulted in maximum rhizogenesis than BAP + NAA and TDZ + NAA-derived shoots. Meta-topolin and NAA combination improved photo-pigments, net rate of photosynthesis, and morpho-anatomy of leaves as compared with other treatments. The rate of photosynthesis, transpiration, and field survival was significantly affected by the types and concentrations of cytokinins used during plantlet development. The present findings unraveled the potential of meta-topolin to alleviate in vitro induced morpho-structural and physiological anomalies and improved survival of micropropagated plantlets of A. vera.

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Acknowledgements

The work was financially supported by the Researchers Supporting Project (RSP-2021/86, King Saud University, Riyadh, Saudi Arabia.

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Authors and Affiliations

  1. Biotechnology Unit, Kanchi Mamunivar Government Institute for Postgraduate Studies and Research, Puducherry, 605 008, India

    M. Manokari, S. Priyadharshini, M. Cokulraj & Mahipal S. Shekhawat

  2. Department of Botany, Siddha Clinical Research Unit, Central Council for Research in Siddha, Palayamkottai, Tamil Nadu, 627 002, India

    M. Manokari

  3. Department of Life Sciences, Presidency University, College Street, Kolkata, 700 073, India

    Abhijit Dey

  4. Department of Botany & Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia

    Mohammad Faisal & Abdulrahman A. Alatar

  5. Department of Plant Pathology, University of Minnesota, Twin cities, Saint Paul, 55108, USA

    Anshu Alok

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Contributions

MM, MSS, PS, and AD: Conceptualization, investigation, methodology. MCR and MF: Data compilation and hardening of the plants. MSS, MM, AAok, and Abdulrahman A. Alatar: Writing of original draft, statistics, and revision of the manuscript. All authors have read and approved the final manuscript.

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Correspondence to Mahipal S. Shekhawat.

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Manokari, M., Priyadharshini, S., Cokulraj, M. et al. Amelioration of Morpho-structural and Physiological Disorders in Micropropagation of Aloe vera L. by Use of an Aromatic Cytokinin 6-(3-Hydroxybenzylamino) Purine. J Plant Growth Regul 42, 4751–4763 (2023). https://doi.org/10.1007/s00344-022-10672-8

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