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Amelioration of Ageing Associated Alterations and Oxidative Inequity in Seeds of Cicer arietinum by Silver Nanoparticles

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Abstract

Metal-based nanoparticles (NPs) have recently been accomplished a great attention worldwide, in various sectors including agriculture due to their beneficial impacts in plant growth, development and stress tolerance. However, it shows dose-dependent response and may vary with type of metal and synthesis procedure followed. Among many, silver nanoparticles (AgNPs) are most frequently used NP in agricultural sector. In the present study, AgNPs were synthesized following both green (gAgNP) and chemical (cAgNP) synthesis processes, characterized by standard methods and were applied to artificially aged Cicer arietinum seeds. Initial characterization of synthesized NPs was done by UV–Visible spectroscopy, and concentrations were calculated as 2.7 nmol for gAgNP, while, 5.8 nmol for cAgNP. Furthermore, the presence of different functional groups in synthesized AgNPs was evaluated by fourier transform infrared spectroscopy (1000 and 4000 cm−1). However, the particle size of synthesized AgNPs was estimated by dynamic light scattering/ zetasizer (90–120 nm) and transmission electron microscopy (15–60 nm). Synthesized NPs were then assessed for their ameliorative efficiencies against accelerated ageing-induced injuries in Cicer arietinum seeds. Experimental results revealed various physiological and biochemical alterations due to accelerated ageing in seeds of Cicer arietinum including the over accumulation of reactive oxygen species and consequent decline in the expressions/ activities of key defensive genes. However, exogenous application of AgNPs provided tolerance against ageing-induced damages by compensating the cellular redox homeostasis via up-regulating the levels/ gene expression of antioxidants in Cicer arietinum.

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Acknowledgements

The authors would like to thank Pt. Ravishankar Shukla University, Raipur, and University Grants Commission, New Delhi, for awarding fellowship to Jipsi Chandra under Research Fellowship (No. 79/8/Fin.Sch/2014, dated 16.04.14) and National Fellowship for students of Other Backward Classes (F./2016-17/NFO-2015-17-OBC-CHH-27902) respectively. Authors are also grateful to Department of Science & Technology, New Delhi, for financial support through DST-FIST scheme (Sanction No. 2384/IFD/2014-15, dated 31.07.2014) sanctioned to the School of Studies in Biotechnology.

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Authors and Affiliations

  1. School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur, 492 010, India

    Jeabunnisha Khan, Jipsi Chandra, Roseline Xalxo & S. Keshavkant

  2. School of Studies in Chemistry, Pt. Ravishankar Shukla University, Raipur, 492 010, India

    Jyoti Korram & Manmohan L. Satnami

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  1. Jeabunnisha Khan
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  6. S. Keshavkant
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Contributions

SK conceptualized the project, designed the experimental protocols, and finalized the manuscript draft. JK and MLS helped in synthesis and characterization of silver nanoparticles. JK, RX and JC performed the experiments, generated and analyzed the data, and drafted the manuscript. All the authors read and approved the final manuscript.

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Correspondence to S. Keshavkant.

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Khan, J., Chandra, J., Xalxo, R. et al. Amelioration of Ageing Associated Alterations and Oxidative Inequity in Seeds of Cicer arietinum by Silver Nanoparticles. J Plant Growth Regul 40, 1341–1351 (2021). https://doi.org/10.1007/s00344-020-10193-2

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