Skip to main content
SpringerLink
Log in

Translocation, Leaf Distribution, and Nutritional Status of Manganese in Cucumber Plants as Affected by Foliar Application of Exogenous Amino Acids

  • Published:
Journal of Plant Growth Regulation Aims and scope Submit manuscript

Abstract

The present study was carried out to investigate the effect of exogenous amino acids (AAs) on leaf apoplastic retention and symplastic absorption, leaf distribution, translocation, and nutritional status of manganese (Mn) in cucumber plants. Results showed that application of AAs increased the symplastic to apoplastic Mn proportion in leaves of cucumber. Furthermore, a greater part of Mn applied with AAs accumulated in the internal area of newly growing leaves suggesting the transport of Mn mostly in the phloem in the presence of AAs. In contrast, Mn preferentially accumulated in the marginal area of treated leaves indicating the Mn movement in xylem according to the transpiration stream. The AAs used in the present study also increased the relative translocation of Mn from treated leaves to the upper leaves and roots. More time was needed for long-distance translocation of Mn from treated leaves to the upper leaves than that to the roots. Application of AAs mainly increased the transport of Mn in the phloem. The leaf PO (peroxidase) activity was the better marker to index the nutritional status of Mn than PPO (polyphenoloxidase) activity. In conclusion, exogenous AAs especially lysine (Lys) promoted the mobility and bioavailability of applied Mn as foliar spray.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

Abbreviations

AA:

Amino acid

Ctrl:

Control

Lys:

Lysine

Met:

Methionine

PO:

Peroxidase

PPO:

Polyphenoloxidase

References

  • Aliyu HN, Na'aliya J (2012) Potentiometric studies on essential metal (II) amino acid complexes. IRJPP 2(2):76–80

    Google Scholar 

  • Álvarez-Fernández A, Díaz-Benito P, Abadía A, López-Millán AF, Abadía J (2014) Metal species involved in long distance metal transport in plants. Front Plant Sci 105:1–20

    Google Scholar 

  • Aravind P, Prasad MNV (2005) Cadmium-induced toxicity reversal by zinc in Ceratophyllum demersum L. (a free floating aquatic macrophyte) together with exogenous supplements of amino-and organic acids. Chemosphere 611:720–733

    Google Scholar 

  • Cerdán M, Sánchez-Sánchez A, Oliver M, Juárez M, Sánchez-Andreu JJ (2009) Effect of foliar and root applications of amino acids on iron uptake by tomato plants. In: Krasteva L, Panayotov N (ed) ISHS 2009, VI Balkan symposium on vegetables and potatoes, Acta Hort. 830, pp 481–488.

  • Cerdán M, Sánchez-Sánchez A, Jordá JD, Juárez M, Sánchez-Andreu J (2013) Effect of commercial amino acids on iron nutrition of tomato plants grown under lime-induced iron deficiency. J Plant Nutr Soil Sci 176:859–866

    Google Scholar 

  • Charnel A, Andreani AM, Eloy JF (1981) Distribution of foliar applied boron measured by spark source mass spectrometry and laser-probe mass spectrography. Plant Physiol 67:457

    Google Scholar 

  • Chen L, Bush DR (1997) LHT1, a lysine- and histidine-specific amino acid transporter in Arabidopsis. Plant Physiol 115:1127–1134

    CAS  PubMed Central  PubMed  Google Scholar 

  • Dordas Ch (2008) Role of nutrients in controlling plant diseases in sustainable agriculture: A review. Agron Sustain Develop 28:33–46

    CAS  Google Scholar 

  • Ducic T, Polle A (2005) Transport and detoxification of manganese and copper in plants. Braz J Plant Physiol 17:103–112

    CAS  Google Scholar 

  • Eskandari S, Khoshgoftarmanesh AH, Sharifnabi B (2018) The effect of foliar-applied manganese in mineral and complex forms with amino acids on certain defense mechanisms of cucumber (Cucumis sativus L.) against powdery mildew. J Plant Growth Regul. 37(2):481–490.

    CAS  Google Scholar 

  • Fageria NK, Barbosa Filho MP, Moreira A, Guimarães CM (2009) Foliar fertilization of crop plants. J Plant Nutr 32:1044–1064

    CAS  Google Scholar 

  • Fecht-Christoffers MM, Führs H, Braun HP, Horst WJ (2006) The role of H2O2-producing and H2O2-consuming peroxidases in the leaf apoplast of Vigna unguiculata L. in manganese tolerance. Plant Physiol 140:1451–1463

    CAS  PubMed Central  PubMed  Google Scholar 

  • Ferrandon M, Chamel A (1988) Cuticular retention, foliar absorption and translocation of Fe, Mn and Zn supplied in organic and inorganic form. J Plant Nutr 11(3):247–263

    CAS  Google Scholar 

  • Fischer WN, Kwart M, Hummel S, Frommer WB (1995) Substrate specificity and expression profile of amino acid transporters (AAPs) in Arabidopsis. J Biol Chem 270:16315–16320

    CAS  PubMed  Google Scholar 

  • Gonçalves AC Jr, Nacke H, Schwantes D, Coelho GF (2014) Heavy metal contamination in Brazilian agricultural soils due to application of fertilizers. In: Hernandez Soriano MC (ed) Environmental risk assessment of soil contamination. Ontech open, Rijeka, pp 105–135

    Google Scholar 

  • Guerra-Guimarães L, Vieira A, Chaves I, Pinheiro C, Queiroz V, Ricardo RJ (2014) Effect of greenhouse conditions on the leaf apoplastic proteome of Coffea arabica plants. J Proteom 104:128–139

    Google Scholar 

  • Hammerschmidt R, Nuckles EM, Kuc J (1982) Association of enhanced peroxidase activity with induced systemic resistance of cucumber to Colletotrichum lagenarium. Physiol Plant Pathol 20:73–82

    CAS  Google Scholar 

  • Hauck M, Paul A, Gross S, Raubuch M (2003) Manganese toxicity in epiphytic lichens: chlorophyll degradation and interaction with iron and phosphorus. Environ Exp Bot 49:181–191

    CAS  Google Scholar 

  • Hirner A, Ladwig F, Stransky H, Okumoto S, Keinath M, Harms A et al (2006) Arabidopsis LHT1 is a high-affinity transporter for cellular amino acid uptake in both root epidermis and leaf mesophyll. Plant Cell 18:1931–1946

    CAS  PubMed Central  PubMed  Google Scholar 

  • Hu Y, Schmidhalter U (2005) Drought and salinity: A comparison of their effects on mineral nutrition of plants. J Plant Nutr Soil Sci 168:541–549

    CAS  Google Scholar 

  • Inouhe M, Huang H, Chaudhary SK, Gupta DK (2012) Heavy metal bindings and their interactions with thiol peptides and other biological ligands in plant cells. In: Gupta DK, Sandalio LM (eds) Metal toxicity in plants: perception, signaling and remediation. Springer, Berlin, pp 1–21

    Google Scholar 

  • Kamiya T, Maeshima M (2004) Residues in internal repeats of the rice cation/H+ exchanger are involved in the transport and selection of cations. J Biol Chem 279:812–819

    CAS  PubMed  Google Scholar 

  • Lidon FC, Teixeira MG (2000) Oxy radicals production and control in the chloroplast of Mn-treated rice. Plant Sci 152:7–15

    CAS  Google Scholar 

  • Liu X, Bush D (2006) Expression and transcriptional regulation of amino acid transporters in plants. Amino Acids 30:113–120

    PubMed  Google Scholar 

  • Maini P (2006) The experience of the first bio stimulant, based on amino acids and peptides: a short retrospective review on the laboratory researches and the practical results. Fertilitas Agrorum 1:29–43

    Google Scholar 

  • Mayer AM, Harel E, Shaul RB (1965) Assay of catechol oxidase-a critical comparison of methods. Phytochemistry 5(4):783–789

    Google Scholar 

  • Millaleo R, Reyes DM, Ivanov AG, Mora ML, Alberdi MA (2010) Manganese as essential and toxic element for plants transport, accumulation and resistance mechanisms. J Soil Sci Plant Nutr 10(4):470–481

    Google Scholar 

  • Mills HA, Jones JB Jr (1996) Plant analysis handbook II: a practical sampling, preparation, analysis, and interpretation guide. Micro-Macro Publishing, Athens, GA

    Google Scholar 

  • Mnasri M, Ghabriche R, Fourati E, Zaier H, Sabally K, Barrington S, Lutts S, Abdelly C, Ghnaya T (2015) Cd and Ni transport and accumulation in the halophyte Sesuvium portulacastrum: implication of organic acids in these processes. Front Plant Sci 6:156

    PubMed Central  PubMed  Google Scholar 

  • Moreno DA, Vfllora G, Pulgar G, Romero L (2000) Effect of nitrogen and potassium supply on concentration of iron and manganese and activities of catalase, peroxidase and aconitase in pepper plants. J Plant Nutr 23:1787–1795

    CAS  Google Scholar 

  • Moreno DA, Vfllora G, Romero L (2003) Variations in fruit micronutrient contents associated with fertilization of cucumber with macronutrients. Sci Hort 97:121–127

    CAS  Google Scholar 

  • Mousavi SR, Shahsavari M, Rezaei M (2011) A general overview on manganese (Mn) importance for crops production. AJBAS 5(9):1799–1803

    Google Scholar 

  • Muhling KH, Sattelmacher B (1995) Apoplastic ion concentration of intact leaves of field bean (Vicia faba) as influenced by ammonium and nitrate nutrition. J Plant Physiol 147:81–86

    Google Scholar 

  • Nasholm T, Kielland K, Ganeteg U (2009) Uptake of organic nitrogen by plants. New Phytol 182:31–48

    PubMed  Google Scholar 

  • Page V, Feller U (2015) Heavy metals in crop plants: Transport and redistribution processes on the whole plant level. Agronomy 5:447–463

    CAS  Google Scholar 

  • Page V, Weisskopf L, Feller U (2006) Heavy metals in white lupin: uptake, root-to-shoot transfer and redistribution within the plant. New Phytol 171:329–341

    CAS  PubMed  Google Scholar 

  • Pedas P, Husted S, Skytte K, Schjoerring JK (2011) Elevated phosphorus impedes manganese acquisition by barley plants. Front Plant Sci 37(2):1–12

    Google Scholar 

  • Pittman JK, Cheng NH, Shigaki T, Kunta M, Hirschi KD (2004) Functional dependence on calcineurin by variants of the Saccharomyces cerevisiae Ca2+/H+ exchanger Vcx1p. Mol Microbiol 54:1104–1116

    CAS  PubMed  Google Scholar 

  • Popko M, Michalak I, Wilk R, Gramza M, Chojnacka K, Górecki H (2018) Effect of the new plant growth biostimulants based on amino acids on yield and grain quality of winter wheat. Molecules 23(470):1–13

    Google Scholar 

  • Renella G, Landi L, Nannipieri P (2004) Degradation of low molecular weight organic acids complexed with heavy metals in soil. Geoderma 122:311–315

    CAS  Google Scholar 

  • Rengel Z (2001) Xylem and phloem transport of micronutrients. In: Horst WJ, et al. (eds) Plant nutrition; developments in plant and soil sciences. Kluwer Academic, Netherlands, pp 628–629

    Google Scholar 

  • Roosta HR, Mohsenian Y (2012) Effects of foliar spray of different Fe sources on pepper (Capsicum annum L.) plants in aquaponic system. Sci Hort 146:182–191

    CAS  Google Scholar 

  • Sánchez-Sánchez A, Juárez M, Sánchez-Andreu J, Jordá J, Bermúdez D (2005) Use of humic substances and amino acids to enhance iron availability for tomato plants from applications of the chelate FeEDDHA. J Plant Nutr 28:1877–1886

    Google Scholar 

  • Sattelmacher B (2001) The apoplast and its significance for plant mineral nutrition. New Phytol 149:167–192

    CAS  PubMed  Google Scholar 

  • Shahid M, Dumat C, Khalid S, Schreck E, Xiong T, Niazi NKh (2016) Foliar heavy metal uptake, toxicity and detoxification in plants: A comparison of foliar and root metal uptake. J Hazard Mater 325:36–58

    PubMed  Google Scholar 

  • Shigaki T, Pittman JK, Hirschi KD (2003) Manganese specificity determinants in the Arabidopsis metal/H+ antiporter CAX2. J Biol Chem 278:6610–6617

    CAS  PubMed  Google Scholar 

  • Silber A, Bar-Tal A, Levkovitch I, Bruner M, Yehezkel H, Shmuel D, Cohen S, Matan E, Karni L, Aktas H, Turhan E, Aloni B (2009) Manganese nutrition of pepper (Capsicum annuum L.): Growth, Mn uptake and fruit disorder incidence. Sci Hort 12:197–203

    Google Scholar 

  • Svennerstam H, Ganeteg U, Bellini C, Näsholm T (2007) Comprehensive screening of Arabidopsis mutants suggests the Lysine Histidine Transporter 1 to be involved in plant uptake of amino acids. Plant Physiol 143:1853–1860

    CAS  PubMed Central  PubMed  Google Scholar 

  • Tegeder M (2014) Transporters involved in source to sink partitioning of amino acids and ureides: opportunities for crop improvement. J Exp Bot 65(7):1865–1878

    CAS  PubMed  Google Scholar 

  • Flowers TJ, Yeo AR (2007) The driving forces for water and solute movement. In: Yeo AR, Flowers TJ (eds) Plant solute transport. Blackwell Publishing Ltd., Hoboken, pp 29–46

    Google Scholar 

  • Wójcik P (2004) Uptake of mineral nutrients from foliar fertilization (Review). Fruit Ornam. Plant Res 12:201–218

    Google Scholar 

  • Xu X, Shi J, Chen Y, Chen X, Wang H, Perera A (2006) Distribution and mobility of manganese in the hyperaccumulator plant Phytolacca acinosa Roxb, (Phytolaccaceae). Plant Soil 285:323–331

    CAS  Google Scholar 

  • Zhang H, Gilbert ER, Zhang K, Ding X, Luo Y, Wang J, Zeng Q, Bai S (2015) Uptake of manganese from manganese–lysine complex in the primary rat intestinal epithelial cells. J Anim Physiol Anim Nutr (Berl) 101(1):147–158

    Google Scholar 

  • Zhou Zh, Zhou J, Li R, Wang H, Wang J (2007) Effect of exogenous amino acids on Cu uptake and translocation in maize seedlings. Plant Soil 292:105–117

    CAS  Google Scholar 

Download references

Acknowledgements

This research did not receive any specific funding.

Author information

Authors and Affiliations

  1. Department of Soil Science, College of Agriculture, Isfahan University of Technology, Isfahan, Iran

    Eskandari Samane

Authors
  1. Eskandari Samane
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Eskandari Samane.

Ethics declarations

Conflict of interest

The author declares that they have no conflicts of interest.

Research Involving Human and Animal Rights

This work is not against human and animal rights.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 46 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Samane, E. Translocation, Leaf Distribution, and Nutritional Status of Manganese in Cucumber Plants as Affected by Foliar Application of Exogenous Amino Acids. J Plant Growth Regul 39, 1191–1204 (2020). https://doi.org/10.1007/s00344-019-10053-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00344-019-10053-8

Keywords

Search

Navigation