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Cadmium Alters Mitochondrial Membrane Potential, Inhibits Electron Transport Chain Activity and Induces Callose Deposition in Rice Seedlings

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Abstract

In the present study, seedlings of indica rice (Oryza sativa L.) cultivars were subjected to 100 and 200 µM Cd(NO3)2 treatment in hydroponics for 10 days which resulted in a marked decline in the growth of the seedlings compared to untreated controls. The integrity of the root cell plasma membrane was significantly decreased and high callose deposition in roots was observed. We observed that mitochondrial functioning was adversely affected in Cd-treated seedling marked by alteration of mitochondrial membrane potential and inhibition of electron transport chain activity. In particular, the activity of the electron transport chain Complex II was more inhibited with Cd treatment compared to the activities of Complex I and Complex IV. Transmission electron microscopic (TEM) analysis of rice leaves from control and Cd-treated seedlings showed significant alteration in membrane composition. Cd treatment at 100 and 200 µM resulted in partial to full disintegration of the mitochondrial membrane, respectively. The increased callose deposition might be a defense response against Cd toxicity. The findings indicate that Cd exposure to rice seedlings causes damage to mitochondria and inhibition of mitochondrial electron transport activity which would ultimately contribute to inhibited growth of rice plants in Cd-polluted soils.

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Acknowledgements

The work reported in this manuscript was financially supported by University Grants Commission, New Delhi in the form of a Major Research Project (No. 40-662/2013). RKS is grateful to Banaras Hindu University for providing a Research Fellowship to him. The authors acknowledge Advanced Instrumentation Research Facility (AIRF), Jawahar Lal Nehru University, New Delhi for providing TEM facility. We also acknowledge Prof S. C. Lakhotia from Department of Zoology, BHU for providing confocal microscope facility. Authors acknowledge Prof. A. M. Kayastha, Coordinator School of Biotechnology, B.H.U. for providing fluorescent spectrophotometer facility. The help provided by Mr. Abhinav Joshi, University of Geneva; Dr. Shikha Chouhan, Department of Biochemistry, BHU and Ms. Susan Westfall, MacGill University, Canada while conducting this work is deeply acknowledged.

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  1. Department of Biochemistry, Institute of Science, Banaras Hindu University, Varanasi, 221005, India

    Rajneesh Kumar Srivastava, Ritika Rajpoot, Poonam Pandey, Anjana Rani & R. S. Dubey

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  1. Rajneesh Kumar Srivastava
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  2. Ritika Rajpoot
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  3. Poonam Pandey
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  4. Anjana Rani
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  5. R. S. Dubey
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Correspondence to R. S. Dubey.

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Srivastava, R.K., Rajpoot, R., Pandey, P. et al. Cadmium Alters Mitochondrial Membrane Potential, Inhibits Electron Transport Chain Activity and Induces Callose Deposition in Rice Seedlings. J Plant Growth Regul 37, 335–344 (2018). https://doi.org/10.1007/s00344-017-9726-2

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  • DOI: https://doi.org/10.1007/s00344-017-9726-2

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