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Kinetin Regulates UV-B-Induced Damage to Growth, Photosystem II Photochemistry, and Nitrogen Metabolism in Tomato Seedlings

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Abstract

Cytokinins are a class of plant growth regulators that regulate several developmental processes in plants, and recently their role in counteracting the deleterious effects of abiotic stresses has been noted. The impacts of kinetin (10 µM, KN; an artificial cytokinin) on growth, photosystem II photochemistry, and nitrogen metabolism in tomato seedlings exposed to two levels (UV-B1, ambient+ 1.2 kJ m−2 day−1, and UV-B2, ambient+ 2.4 kJ m−2 day−1) of enhanced UV-B radiation were analyzed under open field condition. The growth, pigment contents, carbonic anhydrase activity, photosynthetic O2 yield, and values of chlorophyll a fluorescence parameters: F v/F 0, F v/F m or φP0, ψ 0, φE 0, and PIABS declined, whereas the values of energy flux parameters (ABS/RC, TR0/RC, ET0/RC, and DI0/RC) of PS II, efficiency of water splitting complex (F 0/F v), and respiratory rate of O2 uptake increased under UV-B stress. Likewise, UV-B exposure at both doses significantly inhibited the activity of enzymes involved in nitrogen metabolism: nitrate reductase, nitrite reductase, glutamine synthetase, and glutamate synthase. In contrast, an enhancing effect on glutamate dehydrogenase activity was observed under UV-B stress. Exogenous KN resulted in a significant attenuation in UV-B-induced negative effects on growth, pigments, photosynthesis, and nitrogen metabolism. The study concludes that exogenous KN improved the growth performance of tomato seedlings by attenuating the damaging effects of UV-B radiation on photochemistry of PS II and nitrogen metabolism, and the alleviating effect against the low dose (UV-B1) of UV-B was more pronounced.

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Acknowledgements

The financial support provided by Indian Council of Medical and Research, New Delhi, India to Gausiya Bashri as Senior Research Fellow is acknowledged. The financial support of the University Grants Commission, New Delhi to Madhulika Singh, Jitendra Kumar (RGNF-SRF), and Dr. Rohit Kumar Mishra (UGC-Dr. D.S. Kothari PDF) is also acknowledged.

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Authors and Affiliations

  1. Ranjan Plant Physiology and Biochemistry Laboratory, Department of Botany, University of Allahabad, Allahabad, 211002, India

    Gausiya Bashri, Madhulika Singh, Rohit Kumar Mishra, Jitendra Kumar & Sheo Mohan Prasad

  2. Government Ramanuj Pratap Singhdev Postgraduate College, Koriya, Baikunthpur, Chhattisgarh, 497335, India

    Vijay Pratap Singh

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  1. Gausiya Bashri
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Correspondence to Sheo Mohan Prasad.

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Bashri, G., Singh, M., Mishra, R.K. et al. Kinetin Regulates UV-B-Induced Damage to Growth, Photosystem II Photochemistry, and Nitrogen Metabolism in Tomato Seedlings. J Plant Growth Regul 37, 233–245 (2018). https://doi.org/10.1007/s00344-017-9721-7

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