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Microarray-based Analysis of Tomato miRNA Regulated by Botrytis cinerea

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Abstract

Botrytis cinerea Pers.:Fr. is an important pathogen in tomato plants that causes stem rot of tomatoes grown indoors for an extended period. MicroRNAs (miRNAs) have recently been reported as a class of gene expression regulators linked to stress responses; however, data on the role of miRNAs in plant responses to biotic stresses are still limited. In this study, three Botrytis stress-responsive miRNAs were identified using microarray analysis and the effects of Botrytis infection were surveyed in tomatoes. Two downregulated miRNAs and one upregulated miRNA were detected. These stress-responsive miRNAs regulated metabolic, morphological, and physiological adaptations of tomato seedlings at the post-transcriptional level. The presence of stress-related elements in the miRNA promoter regions further supports our results. These findings extend the current view about miRNAs as ubiquitous regulators under stress conditions.

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Acknowledgments

This work was supported by the Province Natural Science Foundation of Shaanxi (grant No. SJ082ZT04), the Natural Science Foundation of China (grant No. 31000913), the Doctoral Fund of Ministry of Education of China (grant No. 20100204120024), and the Foundation of Young Aged Academic Backbone of the Northwest A&F University in China.

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Authors and Affiliations

  1. College of Life Sciences, Northwest A&F University, Yangling, Shannxi, 712100, China

    Weibo Jin, Liang Xiao, Guangwang Liang, Yuxian Zhen, Zekun Guo & Aiguang Guo

  2. College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, 712100, China

    Fangli Wu

  3. Key Laboratory for Molecular Biology of Agriculture, Shaanxi, 712100, China

    Weibo Jin & Guangwang Liang

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  1. Weibo Jin
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  2. Fangli Wu
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  3. Liang Xiao
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Correspondence to Weibo Jin.

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Jin, W., Wu, F., Xiao, L. et al. Microarray-based Analysis of Tomato miRNA Regulated by Botrytis cinerea . J Plant Growth Regul 31, 38–46 (2012). https://doi.org/10.1007/s00344-011-9217-9

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