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The Location of the Chickpea Cell Wall ßV-Galactosidase Suggests Involvement in the Transition between Cell Proliferation and Cell Elongation

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Abstract

We report the generation of antibodies against a ß-galactosidase (ßV-Gal) from Cicer arietinum and the subsequent immunolocalization of the protein in different parts and developmental stages of the plant. ßV-Gal is a cell wall protein encoded by the CanBGal-5 gene, which belongs to a family of at least four ß-galactosidase genes in chickpea. We have previously reported that CanBGal-5 transcripts are located in organs with high elongation and cell division rates, such as meristematic hooks, very young epicotyls, and apical internodes. ßV-Gal protein is the only studied chickpea ß-galactosidase widely present in meristematic hooks, mainly in the meristematic apical zone. These results agree with the previously reported transcription pattern of CanBGal-5 and may reflect its involvement in cell wall modifications during the final stages of cell proliferation, leading to the establishment of an expanding cell wall. The location of ßV-Gal in the cell wall of procambium cells and in pericycle cells of the developing lateral roots also supports the involvement of ßV-Gal in this process. During seedling and plant growth, the highest levels of βV-Gal protein were detected in the youngest actively growing epicotyls and in the apical growing internodes. Thus, protein levels pointed to a relationship between βV-Gal and the events occurring in the cell wall during the early stages of development. Immunolocalization studies in different zones of epicotyls and radicles suggest a role for ßV-Gal in cell elongation.

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Acknowledgments

This work was supported by a grant from the Ministerio de Educación y Ciencia, Spain (BFU2006-09786), and by a grant from the Junta de Castilla y León (SA103A07).

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Authors and Affiliations

  1. Departamento de Fisiología Vegetal, Centro Hispano Luso de Investigaciones Agrarias, Universidad de Salamanca, Plaza Doctores de la Reina s/n, Campus Miguel Unamuno, 37007, Salamanca, Spain

    Ignacio Martín, Teresa Jiménez, Emilia Labrador & Berta Dopico

  2. Departamento de Fisiología Vegetal, Universidad de Santiago de Compostela, Campus de Lugo, 27002, Lugo, Spain

    Josefina Hernández-Nistal

Authors
  1. Ignacio Martín
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  2. Teresa Jiménez
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  3. Josefina Hernández-Nistal
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  4. Emilia Labrador
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  5. Berta Dopico
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Correspondence to Berta Dopico.

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Martín, I., Jiménez, T., Hernández-Nistal, J. et al. The Location of the Chickpea Cell Wall ßV-Galactosidase Suggests Involvement in the Transition between Cell Proliferation and Cell Elongation. J Plant Growth Regul 28, 1–11 (2009). https://doi.org/10.1007/s00344-008-9067-2

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  • DOI: https://doi.org/10.1007/s00344-008-9067-2

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