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Effects of chilled storage and cryopreservation on sperm characteristics, antioxidant enzyme activities, and lipid peroxidation in Pacific cod Gadus microcephalus

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Abstract

The present study evaluated the effects of chilled storage and cryopreservation on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod Gadus macrocephalus. Sperm motility and the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (Gr), and lipid peroxidation (measured via malondialdehyde (MDA) content) were determined after the milt was stored at 4°C for 12 h, cryopreserved without cryoprotectant in 12% propylene glycol (PG), cryopreserved in 12% PG+0.1 mol/L trehalose, or cryopreserved in 12% PG spermatozoa but centrifuged to decant the supernatant prior to cryopreservation (only sperm cells were cryopreserved). After chilled storage or cryopreservation, the SOD, CAT and GPx activities were reduced in sperm cells and increased in seminal plasma in almost all treatments; sperm motility parameters were also decreased. However, the addition of trehalose into the cryoprotectant could significantly improve the postthaw sperm quality as revealed by the sperm average path velocity. This improvement might be attributed to the function of trehalose in scavenging reactive oxygen species. Chilled storage and cryopreservation had significant effects on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod.

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Authors and Affiliations

  1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Xueying Wang  (王学颖), Yifan Liu  (柳意樊), Qinghua Liu  (刘清华) & Jun Li  (李军)

  2. College of Earth Science, University of Chinese Academy of Sciences, Beijing, 100049, China

    Xueying Wang  (王学颖)

  3. College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao, 266109, China

    Xuehui Shi  (史雪辉)

  4. National & Local Joint Engineering Laboratory of Ecological Mariculture, Chinese Academy of Sciences, Qingdao, 266071, China

    Qinghua Liu  (刘清华) & Jun Li  (李军)

  5. Marine Biology Institute of Shandong Province, Qingdao, 266104, China

    Daode Yu  (于道德) & Shuguang Guan  (官曙光)

Authors
  1. Xueying Wang  (王学颖)
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  2. Xuehui Shi  (史雪辉)
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  3. Yifan Liu  (柳意樊)
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  4. Daode Yu  (于道德)
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  5. Shuguang Guan  (官曙光)
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  6. Qinghua Liu  (刘清华)
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  7. Jun Li  (李军)
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Corresponding authors

Correspondence to Qinghua Liu  (刘清华) or Jun Li  (李军).

Additional information

Supported by the National Natural Science Foundation of China (Nos. 31072212, 41076100), the CAS Scientific and Technological Innovation Program for Cross and Cooperative Team, Marine Economy Innovative Development Project (No. 12PYY001SF08), and the National Key Basic Program of Science and Technology Platforms of Aquaculture Stock Resources, Shandong Technology Development Project (No. 2013GHY11509)

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Wang, X., Shi, X., Liu, Y. et al. Effects of chilled storage and cryopreservation on sperm characteristics, antioxidant enzyme activities, and lipid peroxidation in Pacific cod Gadus microcephalus . Chin. J. Ocean. Limnol. 34, 763–771 (2016). https://doi.org/10.1007/s00343-016-5088-z

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  • DOI: https://doi.org/10.1007/s00343-016-5088-z

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