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Bacterial diversity in the intestine of young farmed puffer fish Takifugu rubripes

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Abstract

The aim of the study was to examine the bacterial community associated with the intestinal mucus of young farmed puffer fish Takifugu rubripes. Polymerase chain reaction and partial 16S rDNA sequencing was performed on DNA from bacteria cultivated on Zobell 2216E medium. All the isolates were classified into two phyla—Proteobacteria and Firmicutes. Proteobacteria were the dominant, culturable intestinal microbiota (68.3%). At the genus level, Vibrio, Enterobacter, Bacillus, Pseudomonas, Exiguobacterium, Staphylococcus, Acinetobacter, Pseudoalteromonas and Shewanella were isolated from the intestine, with representatives of the genera Vibrio, Enterobacter and Bacillus accounting for 70.7% of the total. This is the first report of Enterobacter, Bacillus, Exiguobacterium and Staphylococcus as part of the intestinal bacterial microflora in T. rubripes. The profile of the culturable bacterial community differed between samples collected from the same tank at 2-month intervals, as indicated by Bray-Curtis and Sorensen indices, and the impact on the intestinal physiology and health of puffer fish requires further investigation.

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Authors and Affiliations

  1. Key Laboratory of Mariculture and Stock Enhancement in North China’s Sea, Ministry of Agriculture, Dalian Ocean University, Dalian, 116023, China

    Yanyu Li  (李艳宇), Congyao Zhang  (张丛尧), Ying Zhu  (朱莹), Jianfeng Ding  (丁鉴锋) & Yuexin Ma  (马悦欣)

  2. Dalian Tianzheng Industry Co. Ltd., Dalian, 116011, China

    Tao Zhang  (张涛)

Authors
  1. Yanyu Li  (李艳宇)
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  2. Tao Zhang  (张涛)
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  3. Congyao Zhang  (张丛尧)
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  4. Ying Zhu  (朱莹)
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  5. Jianfeng Ding  (丁鉴锋)
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  6. Yuexin Ma  (马悦欣)
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Correspondence to Yuexin Ma  (马悦欣).

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Supported by the Public Science and Technology Research Funds Projects of Ocean (No. 201205025)

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Li, Y., Zhang, T., Zhang, C. et al. Bacterial diversity in the intestine of young farmed puffer fish Takifugu rubripes . Chin. J. Ocean. Limnol. 33, 913–918 (2015). https://doi.org/10.1007/s00343-015-4219-2

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  • DOI: https://doi.org/10.1007/s00343-015-4219-2

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