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Characterization, tissue distribution, and expression of neuropeptide Y in olive flounder Paralichthys olivaceus

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Abstract

Neuropeptide Y (NPY) is a 36-amino acid peptide of the neuropeptide Y family that plays key roles in the regulation of food intake. In this study, we focused on NPY mRNA expression changes around feeding time and during food deprivation in olive flounder. The olive flounder NPY mRNA levels were analyzed in different tissues and a high level of expression was detected in the brain. We also demonstrated a correlation between NPY expression levels in the brain and feeding schedule. NPY expression levels in olive flounder maintained on a daily scheduled feeding regimen increased shortly before feeding and decreased after the scheduled feeding time. Compared with the −1 h group before feeding, NPY expression in the 3 h group after feeding decreased significantly (P<0.05). Food deprivation led to an 81.7% decrease in NPY mRNA levels in the 24 h fasted group (P<0.05) and a 91.7% decrease in the 48 h fasted group (P<0.05). Therefore, our study demonstrates that NPY expression is associated with food intake in olive flounder. This result reveals the function of NPY in regulating food intake and its potential importance in olive flounder aquaculture.

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Authors and Affiliations

  1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Qian Wang  (王倩), Xungang Tan  (谭训刚), Wei Sun  (孙威), Feng You  (尤锋) & Peijun Zhang  (张培军)

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Qian Wang  (王倩)

  3. Department of Biochemistry and Molecular Biology, School of Medicine, University of Maryland, 701 E. Pratt St, Baltimore, MD, 21202, USA

    Shaojun Du  (杜少军)

Authors
  1. Qian Wang  (王倩)
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  2. Xungang Tan  (谭训刚)
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  3. Shaojun Du  (杜少军)
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  4. Wei Sun  (孙威)
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  5. Feng You  (尤锋)
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  6. Peijun Zhang  (张培军)
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Corresponding authors

Correspondence to Xungang Tan  (谭训刚) or Feng You  (尤锋).

Additional information

Supported by the National High Technology Research and Development Program of China (863 Program) (Nos. 2012AA10A408, 2012AA092203), the National Natural Science Foundation of China (No. 31128017), and the National Key Basic Program of Science and Technology-Platforms of Aquaculture Stock Resources (No. 2006DKA30470017)

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Wang, Q., Tan, X., Du, S. et al. Characterization, tissue distribution, and expression of neuropeptide Y in olive flounder Paralichthys olivaceus . Chin. J. Ocean. Limnol. 33, 553–558 (2015). https://doi.org/10.1007/s00343-015-4090-1

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  • DOI: https://doi.org/10.1007/s00343-015-4090-1

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