Abstract
Microsatellites were screened in a backcross family of the Pacific oyster, Crassostrea gigas. Fifteen microsatellite loci were distinguishable and polymorphic with 6 types of allele-combinations. Null alleles were detected in 46.7% of loci, accounting for 11.7% of the total alleles. Four loci did not segregate in Mendelian Ratios. Three linkage groups were identified among 7 of the 15 segregating loci. Fluorescence-based automated capillary electrophoresis (ABI 310 Genetic Analyzer) that used to detect the microsatellite loci, has been proved a fast, precise, and reliable method in microsatellite genotyping.
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Supported by the National Natural Science Foundation of China (NO.40730845, 39825121)
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Li, L., Guo, X. & Zhang, G. Inheritance of 15 microsatellites in the Pacific oyster Crassostrea gigas: segregation and null allele identification for linkage analysis. Chin. J. Ocean. Limnol. 27, 74–79 (2009). https://doi.org/10.1007/s00343-009-0074-3
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DOI: https://doi.org/10.1007/s00343-009-0074-3