Abstract
Lymphocystis disease causes serious economic losses in the fish farming industry. The causative agent of the disease is Lymphocystis disease virus China (LCDV-cn), which has a wide range of hosts. Based on competitive quantitative PCR technology, we established a method to quantify the LCDV-cn in tissue. Results demonstrate that the average amount of LCDV-cn in the peripheral blood of infected flounder with evident tumors is about 106 virions/ml while the average amount in those flounder with no evident tumor but cultured with the flounder with evident tumor is about 104 virions/ml. No virus was found in the negative samples of flounder.
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Supported by the National High Technology Research and Development Program of China (863 Program, No. 2003AA622030, 2006AA100309).
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Zan, J., Sun, X., Zhang, Z. et al. Application of quantitative PCR method in detection of Lymphocystis disease virus China (LCDV-cn) in Japanese flounder (Paralichthys olivaceus). Chin. J. Ocean. Limnol. 25, 418–422 (2007). https://doi.org/10.1007/s00343-007-0418-9
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DOI: https://doi.org/10.1007/s00343-007-0418-9