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Optimization of medium and cultivation conditions for enhanced exopolysaccharide yield by marine Cyanothece sp. 113

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Abstract

Cyanothece sp. 113 is a unicellular, aerobic, diazotrophic and photosynthetic marine cyanobacterium. The optimal medium for exopolysaccharide yield by the strain was 70.0 g/L of NaCl, and 0.9 g/L of MgSO4 based on the modified F/2 medium for cultivation of marine algae. The optimal cultivation condition for exopolysaccharide yield by this cyanobacterial strain was 29°C, aeration, and continuous illumination at 86.0 μE/M2/S. Under the optimal conditions, over 18.4 g/L of exopolysaccharide was produced within 12 days. This was so far the highest exopolysaccharide yield produced with strains of Cyanothece sp. obtained.

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References

  • Chi, Z., J. Liu, J. Ji and Z. Meng, 2003a. Enhanced conversion of starch to trehalose by a mutant of Saccharomycopsis fibuligera Sdu. J. Biotechnol. 102: 135–141.

    Article  Google Scholar 

  • Chi, Z. and S. Zhao, 2003b. Optimization of medium and cultivation conditions for pullulan production by a new pullulan-producing yeast. Enzyme Microb. Technol. 32: 206–211.

    Article  Google Scholar 

  • De Philippis, R., C. Sili, G. Tassinato, M. Vincenzini and R. Materassi, 1991. Effects of growth conditions on exopolysaccharide production by Cyanospira capsulata. Biores. Technol. 38: 101–104.

    Article  Google Scholar 

  • De Philippis, R., M. C. Margheri, E. Pelosi and S. Ventura, 1993. Exoplysaccharide production by a unicellular cyanobacterium isolated from a hypersaline habitat. J. Appl. Phycol. 5: 387–394.

    Article  Google Scholar 

  • De Philippis, R. and M. Vincenzini, 1998a. Exocellular polysaccharide from cyanobacteria and their possible applications. FEMS Microbiol. Rev. 22: 151–175.

    Article  Google Scholar 

  • De Philippis, R., M. C. Margheri, R. Materassi and M. Vincenzini, 1998b. Potential unicellular cyanobacteria from saline environments as exopolysaccharide producers. Appl. Environ. Microbiol. 64: 1 130–1 132.

    Google Scholar 

  • Hill, D. R., A. Peat and M. Potts, 1997. Biochemistry and structure of the glycan secreted by desiccation-tolerant Nostoc commune. Protoplasma. 182: 126–148.

    Article  Google Scholar 

  • John, G. H., R. K. Noel, H. A. S. Peter, T. S. James and T. W. Stanley, 1994. Group II Oxygenic phototrophic bacteria. In: J. G. Holt(ed): Bergey’s Manual of Determinative Bacteriology (9th edition). Williams and Wilkins, Baltimore. pp. 377–383.

    Google Scholar 

  • Lama, L., B. Nicolaus, V. Calandrelli, M. C. Manca, I. Tomano and A. Gambacorta, 1996. Effect of growth conditions on endo-and exopolymer biosynthesis in Anabaena cylindrica 10C. Phytochemistry 42: 655–659.

    Article  Google Scholar 

  • Mark, A. S., A. Boaz, D. M. John, D. S. S. Steven, N. Suzanne, R. S. Puspa, P. T. Paula and A. S. Louis, 1995. Compositional and toxicological evaluation of the diazotrophic cyanobacterium, Cyanothece sp. strain ATCC 51142. Aquaculture 134: 339–349.

    Article  Google Scholar 

  • Mehta, V. B. and B. S. Vaidya, 1978. Cellular and extracellular polysaccharides of the blue-green alga Nostoc. J. Exp. Bot. 29: 1 423–1 430.

    Article  Google Scholar 

  • Milagros, S. C. L., M. S. Debra and L. A. Sherman, 1997. Transcriptional and translational regulation of nitrogenase in light-dark-and continuous-light-grown cultures of the unicellular cyanobacterium Cyanothece sp. strain ATCC 51142. J. Bacteriol. 179: 3 419–4 327.

    Google Scholar 

  • Moore, B. G. and R. G. Tischer, 1964. Extracellular polysaccharides of algae: effects on life-support systems. Science 145: 568–587.

    Article  Google Scholar 

  • Reddy, K. J., B. W. Soper, J. Tang and R. L. Bradley, 1996. Phenotypic variation in exopolysaccharide production in the marine, aerobic nitrogen-fixing unicellular cyanobacterium Cyanothece sp. World J. Microbiol. Biotechnol. 12: 311–318.

    Article  Google Scholar 

  • Scheegurt, M. A., B. Arieli, S. S. Nielsen, P. R. Trumbo and L. A. Sherman, 1996. Evaluation of Cyanothece sp. ATCC 51142 as a candidate for inclusion in a CELSS. Pergamon. 18: 177–180.

    Google Scholar 

  • Sudo, H., B. J. Grant, H. Takemasa, N. Nakamura and T. Matsunaga, 1995. Sulfated exopolysaccharide production by the halophilic cyanobacterium Aphanocapsa halophytica. Curr. Microbiol. 30: 219–222.

    Article  Google Scholar 

  • Tischer, R. G. and E. B. Davis, 1971. The effects of various nitrogen sources upon the production of exocellular polysaccharide by the blue-green alga Anabaena flos-aquae A-37. J. Exp. Bot. 22: 546–551.

    Article  Google Scholar 

  • Zhao, S. and Z. Chi, 2003. A new pullulan-producing yeast and medium optimization for its exopolysaccharide production. Journal of Ocean University of Qingdao 2: 53–57.

    Google Scholar 

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Correspondence to Chi Zhenming  (池振明).

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Supported by the NSFC (No. 30328021)

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Su, C., Chi, Z. & Lu, W. Optimization of medium and cultivation conditions for enhanced exopolysaccharide yield by marine Cyanothece sp. 113. Chin. J. Ocean. Limnol. 25, 411–417 (2007). https://doi.org/10.1007/s00343-007-0411-3

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  • DOI: https://doi.org/10.1007/s00343-007-0411-3

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