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Continuous-wave stimulated Raman scattering (cwSRS) microscopy

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Abstract

Stimulated Raman scattering (SRS) microscopy is a powerful tool for chemically sensitive non-invasive optical imaging. However, ultrafast laser sources, which are currently employed, are still expensive and require substantial maintenance to provide temporal overlap and spectral tuning. SRS imaging, which utilizes continuous-wave laser sources, has a major advantage, as it eliminates the cell damage due to exposure to the high-intensity light radiation, while substantially reducing the cost and complexity of the setup. As a proof-of-principle, we demonstrate microscopic imaging of dimethyl sulfoxide using two independent, commonly used lasers, a diode-pumped, intracavity doubled 532-nm laser and a He–Ne laser operating at 632.8-nm.

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Acknowledgments

This work was partially supported by the start-up funds available through Texas A&M University. Authors also acknowledge the support of the NIH (Grant #R21EB011703) and the NSF (ECS Grant #0925950, DBI Grant #0964225, and CBET Grant #1066562).

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Authors and Affiliations

  1. Department of Biomedical Engineering, Texas A&M University, College Station, TX, 77843, USA

    Zhaokai Meng, Georgi I. Petrov & Vladislav V. Yakovlev

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  1. Zhaokai Meng
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  2. Georgi I. Petrov
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  3. Vladislav V. Yakovlev
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Correspondence to Zhaokai Meng.

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Meng, Z., Petrov, G.I. & Yakovlev, V.V. Continuous-wave stimulated Raman scattering (cwSRS) microscopy. Appl. Phys. B 112, 99–103 (2013). https://doi.org/10.1007/s00340-013-5405-6

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  • DOI: https://doi.org/10.1007/s00340-013-5405-6

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