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Dy2O3-unpurified hydroxyapatite: a promising thermoluminescent sensor and biomimetic nanotherapeutic

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Abstract

In the present work, nanostructured hydroxyapatite (nano-Hap) was synthesized by following a hydrothermal process using calcium hydroxide and ammonium phosphate. The nano-Hap was unpurified with different amounts of dysprosium oxide (Dy2O3), from 0.5 to 2.0 wt%. Its physicochemical properties were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR). TEM confirmed the formation of rod structures (L/D aspect ratio > 1) with average sizes ranging from ~ 15 to 19 nm in diameter, and ~ 26 to 32 nm in length. The presence of the monoclinic and hexagonal crystalline phases of Hap was confirmed by XRD. The thermoluminescence (TL) studies showed a linear relationship between the Dy2O3 concentration and TL intensity induced by gamma radiation in a dose range from 10 to 200 Gy. The nano-Hap unpurified with 2.0 wt% of Dy2O3 showed the highest TL response. Additionally, in the perspective of exploring the therapeutic potential of Dy2O3-unpurified Hap, the viability of human prostatic epithelial cells was evaluated. The safety of pure Hap was confirmed by performing resazurin/resorufin fluorescence-based assays. A significant toxicity was observed as a function of the impurity content and nanopowders concentration. In summary, the nano-Hap TL results make it a good candidate for ionizing radiation sensoring and, the in vitro test encourages the development of a biomimetic Dy-Hap drug-free nanotherapeutic platform.

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Acknowledgements

Our gratitude to Ph.D. Bernardo Yañez Soto for providing the facilities to evaluate the Hap/cell interaction by fluorescence microscopy at the National Laboratory for Out of Equilibrium Matter Engineering (LANIMFE). In vitro evaluation was partially supported by UASLP (Project/C19-FAI-05-87.87). We would like to thank Ph.D. Mildred Quintana Ruíz and Eng. Celina González Gallegos for their support to measure fluorescence in solution. We also thank CONACYT for supporting the generation of infrastructure through the INFR-2015-251767 project and for the scholarship awarded to Susana López Ortiz belonging to the Doctorate program 0432 in Materials Sciences from the Autonomous University of the State of Hidalgo.

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Authors and Affiliations

  1. Área Académica de Ciencias de La Tierra Y Materiales, Instituto de Ciencias Básicas E Ingeniería, Universidad Autónoma del Estado de Hidalgo, 42184, Pachuca Hidalgo, Mexico

    S. López Ortiz, V. Rodríguez Lugo & M. I. Reyes-Valderrama

  2. Cátedras CONACyT, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, 78210, San Luis Potosí, S.L.P, Mexico

    D. Salado-Leza

  3. Cátedras CONACyT, Coordinación Para La Innovación Y Aplicación de La Ciencia Y La Tecnología, Universidad Autónoma de San Luis Potosí, 78210, San Luis Potosí, S.L.P, Mexico

    L. E. Alcántara-Quintana

  4. Instituto Nacional de Investigaciones Nucleares, 52750, Ocoyoacac Estado de México, Mexico

    P. González-Martínez & D. Mendoza Anaya

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  1. S. López Ortiz
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  2. V. Rodríguez Lugo
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  3. D. Salado-Leza
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Ortiz, S.L., Lugo, V.R., Salado-Leza, D. et al. Dy2O3-unpurified hydroxyapatite: a promising thermoluminescent sensor and biomimetic nanotherapeutic. Appl. Phys. A 127, 893 (2021). https://doi.org/10.1007/s00339-021-05010-w

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