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Femtosecond laser ablation of neurons in C. elegans for behavioral studies

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Abstract

Femtosecond laser ablation selectively dissects subcellular components of the C. elegans neuronal circuit with submicrometer precision for studying the neuronal origins of behavior. We describe the theoretical basis for the high precision of femtosecond laser ablation in the target bulk. Next, we present the experimental setup and a worm rotation technique to facilitate imaging and surgery. We describe the damage caused by different pulse energies on cell bodies and neuronal fibers. Finally, we discuss the regrowth of neuronal fibers after surgery and its impact on behavioral study.

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Authors and Affiliations

  1. School of Engineering and Applied Sciences, Harvard University, 9 Oxford Street, Cambridge, MA, 02138, USA

    Samuel H. Chung

  2. Department of Physics and School of Engineering and Applied Sciences, Harvard University, 9 Oxford Street, Cambridge, MA, 02138, USA

    Eric Mazur

Authors
  1. Samuel H. Chung
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  2. Eric Mazur
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Correspondence to Samuel H. Chung.

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Chung, S.H., Mazur, E. Femtosecond laser ablation of neurons in C. elegans for behavioral studies. Appl. Phys. A 96, 335–341 (2009). https://doi.org/10.1007/s00339-009-5201-7

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  • DOI: https://doi.org/10.1007/s00339-009-5201-7

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