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Influence of sperm dilution and gamete contact time on the fertilization rate of scleractinian corals

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Abstract

This study presents new information on the influence of sperm dilution on the fertilization rates of eight broadcast-spawning scleractinian coral species [three Acropora species and five merulinid species (three genera)]. The presented information nearly doubled the existing information, now totaling 17 species comprising eight acroporid species and nine merulinid species. No obvious differences in the fertilization rates were observed at the family and genus levels; furthermore, the fertilization curve estimated uniquely for Favites pentagona exhibited a strong sigmoid shape, indicating the existence of species-specific variation. In addition, a general fertilization response against sperm dilution was observed for the first time in broadcast-spawning scleractinian corals. The fertilization rate peaked (>75 %) at a sperm concentration of approximately 106 sperm mL−1 (optimal concentration) and rapidly declined to <50 % at a concentration of 104 sperm mL−1. The influence of gamete contact time (10, 30, and 60 min) on fertilization rates was also examined in two Acropora and four merulinid species, at the optimal sperm concentration. No influence of gamete contact time on fertilization rates was observed in two of the examined species (Acropora papillare and Platygyra ryukyuensis), whereas reduced fertilization rates occurred mostly in the 10-min treatment for the other species. These results suggested that broadcast-spawning scleractinian corals can rapidly fertilize, indicating that these corals have a fair chance of achieving high fertilization success in the field under optimal conditions. The sperm concentration values (e.g., 104 sperm mL−1, indicating <50 % fertilization rates) may be useful in estimating the success of in situ fertilization of broadcast-spawning scleractinian corals, particularly in degraded, low-density populations where the degree of fertilization success is of management concern. Information on the fertilization ecology of scleractinian corals remains insufficient, and further study is necessary to understand fertilization dynamics in the field.

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Acknowledgments

The authors thank the staff of the Lyudao Marine Research Station for their support and, particularly, Adriana Santacruz-Castro, Che-Hung Lin, Chih-Hsien Liu, Gina Yang, and Huai-Shin Hsieh for their assistance in the field. Comments from three anonymous reviewers improved the original manuscript. This study was funded by an internal research grant from Biodiversity Research Center, Academia Sinica, and a thematic research grant from Academia Sinica (23-2g).

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Authors and Affiliations

  1. Biodiversity Research Center, Academia Sinica, 128 Academia Road Sec. 2, Nankang, Taipei, 115, Taiwan, ROC

    Yoko Nozawa

  2. Department of Bioresources Engineering, Okinawa National College of Technology, 905 Henoko, Nago-City, Okinawa, 905-2192, Japan

    Naoko Isomura

  3. Faculty of Agriculture, Department of Marine Biology and Environmental Science, University of Miyazaki, Miyazaki, Japan

    Hironobu Fukami

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  1. Yoko Nozawa
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  2. Naoko Isomura
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  3. Hironobu Fukami
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Correspondence to Yoko Nozawa.

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Communicated by Biology Editor Dr. Line K. Bay

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Nozawa, Y., Isomura, N. & Fukami, H. Influence of sperm dilution and gamete contact time on the fertilization rate of scleractinian corals. Coral Reefs 34, 1199–1206 (2015). https://doi.org/10.1007/s00338-015-1338-3

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  • DOI: https://doi.org/10.1007/s00338-015-1338-3

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