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Construction and characterization of a sheep BAC library of three genome equivalents

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Abstract

A sheep BAC library of over three genome equivalents was constructed and arrayed in superpools and row, column, and plate pools. The library contains 90,000 clones distributed in 39 superpools. The average insert size was estimated at 123 kb. The library was screened by PCR with 77 primer pairs corresponding to ovine microsatellites distributed throughout the genome. The probability of finding a random sequence in the library could be estimated at 0.96.

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Authors and Affiliations

  1. Laboratoire de Génétique biochimique et de Cytogénétique, Département de Génétique Animale, INRA, 78352 Jouy-en-Josas, France, FR

    Daniel Vaiman, Laurent Schibler, Didier Vilette, Anne Oustry-Vaiman & Edmond P. Cribiu

  2. Fondation Jean Dausset-Centre d'Etude du Polymorpisme Humain, 27, rue Juliette Dodu, 75010 Paris, France, FR

    Alain Billault & Catherine Soravito

  3. Laboratoire de Pathologie infectieuse et d'Immunologie, INRA, 37380, Nouzilly, France, FR

    Kamila Tabet-Aoul

Authors
  1. Daniel Vaiman
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  2. Alain Billault
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  3. Kamila Tabet-Aoul
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  4. Laurent Schibler
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  5. Didier Vilette
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  6. Anne Oustry-Vaiman
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  7. Catherine Soravito
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  8. Edmond P. Cribiu
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Received: 2 November 1998 / Accepted: 29 January 1999

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Vaiman, D., Billault, A., Tabet-Aoul, K. et al. Construction and characterization of a sheep BAC library of three genome equivalents. Mammalian Genome 10, 585–587 (1999). https://doi.org/10.1007/s003359901049

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  • DOI: https://doi.org/10.1007/s003359901049

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