Abstract
Recently two main genetic maps [Rohrer et al. Genetics 136, 231 (1994); Archibald et al. Mamm. Genome 6, 157 (1995)] and a cytogenetic map [Yerle et al. Mamm. Genome 6,175 (1995)] for the porcine genome were reported. As only a very few microsatellites are located on the cytogenetic map, it appears to be important to increase the relationships between the genetic and cytogenetic maps. This document describes the regional mapping of 100 genetic markers with a somatic cell hybrid panel. Among the markers, 91 correspond to new localizations. Our study enabled the localization of 14 new markers found on both maps, of 54 found on the USDA map, and of 23 found on the PiGMaP map. Now 21% and 43% of the markers on the USDA and PiGMaP linkage maps respectively are physically mapped. This new cytogenetic information was then integrated within the framework of each genetic map. The cytogenetic orientation of the USDA linkage maps for Chromosomes (Chrs) 3, 8, 9, and 16 and of PiGMaP for Chr 8 was determined. USDA and PiGMaP linkage maps are now oriented for all chromosomes, except for Chrs 17 and 18. Moreover, the linkage group “R” from the USDA linkage map was assigned to Chr 6.
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Andersson, L., Haley, C.S., Ellegren, H., Knott, S.A., Johansson, M., Andersson, K., Andersson-Eklund, L., Edfords-Lilja, I., Fredholm, M., Hansson, I., Hakansson, J., Lundström, K. (1994). Genetic mapping of quantitative trait loci for growth and fatness in pigs. Science 263, 1771–1774.
Archibald, A.L., Brown, J.F., couperwhite, S., Mc Queen, H.A., Nicholson, D.,
Haley, C.S., Coppieters, W., Van de Weghe, A., Stratil, A., Winterø, A.K., Fredholm, M., Larsen, N.J., Nielsen, V.H., Milan, D., Woloszyn, N., Robic, A., Dalens, M., Riquet, J., Gellin, J., Caritez, J.C., Hue, D., Burgaud, G., Ollivier, L., Bidanel, J.P., Vaiman, M., Renard, C, Geldermann, H., Davoli, R., Ruyter, D., Versteger, E.J.M., Groenen, M.A.M., Davies, W., Høyheim, B., Keiserud, A., Andersson, L., Ellegren, H., Johansson, M, Marklund, L., Miller, J.R., Anderson Dear, D.V., Signer, E., Jeffreys, A.J., Moran, C, Le Tissier, P., Rothschild, M.R., Tuggle, C.K., Vaske, D., Helm, J., Liu, H.C., Rahman, A., Yu, T.P., Larson, R.G., Schmitz, C.B. (1995). The PiGMaP consortium linkage map of the pig (Sus scrofa). Mamm. Genome 6, 157–175.
Chevalet, C., Corpet, F. (1986). Statistical decision rules concerning syntheny or independence between markers. Cytogenet. Cell Genet. 43, 132–139.
Cuticchia, A.J., Pearson, P.L. (eds) (1993). Human Gene Mapping 1993. (Baltimore: The Johns Hopkins University Press).
Davies, W., Høyheim, B., Skogtvedt, S. (1995). Alignment of two genetic maps on porcine chromosome 13. Anim. Genet. 26, 119–121.
Ellegren, H., Chowdhary, B.P., Fredholm, M., Høyheim, B., Johansson, M., Nielsen, P.B., Thomsen, P.D., Andersson, L. (1994a). A physically anchored linkage map of pig chromosome 1 uncovers sex- and position-specific recombination rates. Genomics 24, 342–350.
Ellegren, H., Chowdhary, B.P., Johansson, M., Andersson, L. (1994b). Integrating the porcine physical and linkage map using cosmid-derived markers, anim. Genet. 25, 155–164.
Ellegren, H., Chowdhary, B.P., Johansson, M., Marklund, L., Fredholm, M., Gustavsson, I., Andersson, L. (1994c). A primary linkage map of the porcine genome reveals a low rate of genetics recombination. Genetics 137, 1089–199.
Goureau, A., Yerle, M., Schmitz, A., Riquet, J., Milan, D., Pinton, P., Frelat, G., Gellin, J. (1996). Determination of correspondences between human and porcine chromosome segments using bidirectional chromosome painting. Genomics, in press.
Hoppe, B.L., Conti-tronconi, B.M., Horton, R.H. (1992). Gel loading dyes compatibles with PCR. Biotechniques 12, 679–680.
Lahbib-Mansais, Y., Yerle, M, Pinton, P., Gellin, J. (1996). Comparative mapping of Hox clusters and reference anchor loci in pig and other mammals by in situ hybridization. Mamm. Genome, in press.
McQueen, H.A., Haley, C.S., Couperwhite, S., Archibald, A.L. (1995). A linkage map of pig chromosome 10. J. Hered. 86, 228–230.
Milan, D., Woloszyn, N., Yerle, M., Le Roy, P., Bonnet, M., Riquet, J., Lahbib-Mansais, Y., Caritez, J.C., Robic, A., Sellier, P., Elsen, J.M., Gellin, J. (1996). Accurate mapping of the acid meat RN gene on genetic and physical maps of pig Chromosome 15. Mamm. Genome, 7, 41–45.
Pappas, G.J., Polymeropoulos, M.H., Boyle, J.M., Trent, J.M. (1995). Regional assignment of hybrid mapping of 36 expressed sequence tags (ESTs) on human chromosome 6. Genomics 25, 124–129.
Rettenberger, G., Fries, R., Engel, W., Scheit, K.H., Dolf, G., Hameister, H. (1994). Establishment of a partially informative porcine somatic cell hybrid panel and a assignment of the loci for transition protein 2 (TNP2) and protamine 1 (PRM1) to chromosome 3 and polyubiquitin (UBC) to chromosome 14. Genomics 21, 558–566.
Riquet, J., Milan, D., Woloszyn, N., Schmitz, A., Pitel, F., Frelat, G., Gellin, J. (1995). A linkage map with microsatellites isolated from swine flow-sorted chromosome 11. Mamm. Genome 6, 623–628.
Robic, A., Parrou, J.L., Yerle, M., Goureau, A., Dalens, M., Milan, D., Gellin, J. (1995). Pig microsatellites isolated from cosmids revealing polymorphism and localized on chromosomes. Anim. Genet. 26, 1–6.
Rohrer, G.A., Alexander, L.J., Keele, J.W., Smith, T.P., Beattie, C.W. (1994). A microsatellite linkage map of the porcine genome. Genetics 136, 231–245.
Schmitz, A., Chardon, P., Gainche, L, Chaput, B., Guilly, M.N., Frelat, G., Vaiman, M. (1992). Swine chromosomal DNA quantification by bivariate flow karyotyping and karyotype interpretation. Cytometry 132, 703–710.
Troyer, D.L., Goad, D.W., Xie, H., Rohrer, G.A., Alexander, L.J., Beattie, C.W. (1994). Use of direct in situ single-copy (DISC) PCR to physically map five porcine microsatellites. Cytogenet. Cell Genet. 67, 199–204.
Xie, H., Alexander, I.J., Rohrer, G.A., Beattie, C.W., Troyer, D.L. (1995). Use of DISC-PCR and FISH to assign a linkage group to pig chromosome 10. Mamm. Genome 6, 139–141.
Yerle, M., Lahbib-Mansais, Y., Mellink, C., Goureau, A., Pinton, P., Echard, G., Gellin, J., Zijlstra, C., De Haan, N., Bosma, A.A., Chowdhary, B., Gu, F., Gustavson, I., Thomsen, P.D., Christensen, K., Rettenberger, G., Hameister, H., Schmitz, A., Chaput, B., Frelat, G. (1995). The PiGMaP consortium cytogenetic map of the domestic pig (Sus crofa domestica). Mamm. Genome 6, 175–186.
Yerle, M., Echard, G., Robic, A., Mairal, A., Dubut-Fontana, C., Riquet, J., Pinton, P., Milan, D., Gillois, M., Gellin, J. (1996). A somatic cell hybrid panel for pig regional gene mapping characterized by molecular cytogenetics. Cytogenet. Cell Genet., in press.
Zijlstra, C., Bosma, A.A., De Haan, N.A. (1994). Comparative study of pig-rodent somatic cell hybrids. Anim. Genet. 25, 319–327.
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Robic, A., Riquet, J., Yerle, M. et al. Porcine linkage and cytogenetic maps integrated by regional mapping of 100 microsatellites on somatic cell hybrid panel. Mammalian Genome 7, 438–445 (1996). https://doi.org/10.1007/s003359900129
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DOI: https://doi.org/10.1007/s003359900129