Abstract
Comparative chromosome painting with individual human chromosome-specific libraries (CSLs) on cattle metaphase chromosomes delineated 46 homologous chromosomal segments between the two species. Continuous arrangement of these segments on individual cattle chromosomes demonstrates a nearly complete coverage of the bovine karyotype and shows physical boundaries of bovine chromosomal segments homologous to individual human chromosomes. Alignment of the available comparative gene mapping data with the homologous segments strongly supports the detected gross homologies between the karyotypes of the two species. In addition to cattle, four human CSLs were hybridized to sheep metaphase chromosomes also, to further verify the known karyotype homology within the Bovidae. Besides its application to karyotype evolution research, the comparative knowledge provides for rapid expansion of the much needed Type I locus-based bovine gene map.
Similar content being viewed by others
References
Bachmann, K. (1972). Genome size in mammals. Chromosoma 37, 85–93.
Barendse, W., Armitage, S.M., Kossarek, L.M., Shalom, A., Kirkpatrick, B.W., Ryan, A.M., Clayton, D., Li, L., Zhang, N., Grosse, W.M., Weiss, J., Creighton, P., McCarthy, F., Ron, M., Teale, A.J., Fries, R., McGraw, R.A., Moore, S.S., Georges, M., Soller, M., Womack, J.E., Hetzel, D J.S. (1994). A genetic linkage map of the bovine genome. Nature Genet. 6, 227–234.
Beever, J.E., Da, Y., Fischer, S.R., Ron, M., Lewin, H.A. (1994). A genetic map of 9 loci on bovine chromosome 2. Mamm. Genome 5, 542–545.
Bishop, M.D., Kappes, S.M., Keele, J.W., Stone, R.T., Sunden, S.L.F., Hawkins, G.A., Solinas-Toldo, S., Fries, R., Grosz, M.D., Yoo, J., Beattie, C.W. (1994). A genetic linkage map for cattle. Genetics 136, 619–639.
Collins, C., Kuo, W.L., Segraves, R., Pinkel, D., Fuscoe, J., Gray, J.W. (1991). Construction and characterization of plasmid libraries enriched in sequences from single human chromosomes. Genomics 11, 997–1004.
Copeland, N.G., Jenkins, N.A., Gilbert, G.J., Eppig, J.T., Maltais, L.J., Miller, J.C., Dietrich, W.F., Weaver, A., Lincoln, S.E., Steen, R.G., Stein, L.D., Nadeau, J.H., Lander, E.S. (1993). A genetic linkage map of the mouse: current applications and further prospects. Science 262, 57–62.
Eggen, A., Fries, R. (1995). An integrated cytogenetic and meiotic map of the bovine genome. Anim. Genet. 26, 215–236.
Frönicke, L., Chowdhary, B.P., Scherthan, H., Gustavsson, I. (1996). A comparative map of the porcine and human genomes demonstrates ZOO-FISH and gene mapping-based chromosomal homologies. Mamm. Genome 7, 285–290.
Harris, P., Boyd, E., Young, B.D., Ferguson-Smith, M.A. (1986). Determination of the DNA content of the human chromosomes by flow cytometry. Cytogenet. Cell Genet. 41, 14–21.
Hayes, H. (1995). Chromosome painting with human chromosome-specific DNA libraries reveals the extent and distribution of conserved segments in bovine chromosomes. Cytogenet. Cell Genet. 71, 168–174.
Hayes, H., Petit, E., Dutrillaux, B. (1991). Comparison of RBG-banded karyotypes of cattle, sheep and goats. Cytogenet. Cell Genet. 57, 51–55.
Iannuzzi, L., DiMeo, G.P. (1995). Chromosomal evolution in bovids: a comparison of cattle, sheep and goat G- and R-banded chromosomes and cytogenetic divergences among cattle, goat and river buffalo sex chromosomes. Chromosome Res. 3, 291–299.
ISCNDA (1989). International System for Cytogenetic Nomenclature of Domestic Animals. Cytogenet. Cell Genet. 53, 65–79.
Kaftanovskaya, H.M., Serov, O.L. (1994). High resolution GTG-banded chromosomes of cattle, sheep and goat: a comparative study. J. Hered. 85, 395–400.
Lin, C.C., Newton, D.R., Church, R.B. (1977). Identification and nomenclature for G-banded bovine chromosomes. Can. J. Genet. Cytol. 19, 271–282.
Morton, N.E. (1991). Parameters of the human genome. Proc. Natl. Acad. Sci. USA 88, 7474–7476.
Nadeau, J.H., Davisson, M.T., Doolittle, D.P., Grant, P., Hillyard, A.L., Kosowsky, M.R., Roderick, T.H. (1992). Comparative map for mice and humans. Mamm. Genome 3, 480–491.
Nowak, R. (1994). Mining treasures from ‘junk DNA’. Science 263, 608–610.
O’Brien, S.J., Seuanez, H.N., Womack, J.E. (1988). Mammalian genome organization: an evolutionary view. Annu. Rev. Genet. 22, 323–351.
O’Brien, S.J., Womack, J.E., Lyons, L.A., Moore, K.J., Jenkins, N.A., Copeland, N.G. (1993). Anchored reference loci for comparative genome mapping in mammals. Nature Genet. 3, 103–112.
Reading Conference, 1976 (1980). Proceedings of the First International Conference for the Standardization of Banded Karyotypes of Domestic Animals. Hereditas 92, 145–162.
Rettenberger, G., Klett, C., Zechner, U., Kunz, J., Vogl, W., Hameister, H. (1995). Visualization of the conservation of synteny between humans and pigs by heterologous chromosomal painting. Genomics 26, 372–378.
Scherthan, H., Cremer, T., Arnason, U., Weier, H.-U, Lima-de-Faria, A., Frönicke, L. (1994). Comparative chromosome painting discloses homologous segments in distantly related mammals. Nature Genet. 6, 342–347.
Solinas-Toldo, S., Lengauer, C., Fries, R. (1995). Comparative genome map of human and cattle. Genomics 27, 489–496.
Tiersch, T.R., Chandler, R.W., Wachtel, S.S., Elias, S. (1989). Reference standards for flow cytometry and application in comparative studies of nuclear DNA content. Cytometry 10, 706–710.
Vaiman, D., Mercier, D., Eggen, A., Bahri-Darwich, I., Grohs, C., Cribiu, E.P., Dolf, G., Oustry, A., Guerin, G., Leveziel, H. (1994). A genetic and physical map of bovine chromosome 11. Mamm. Genome 5, 553–556.
Vooijs, M., Yu, L.C., Tkachuk, D., Pinkel, D., Johnson, D., Gray, J.W. (1993). Libraries for each human chromosome, constructed from sorterenriched chromosomes by using linker-adaptor PCR. Am. J. Hum. Genet. 52, 586–597.
Womack, J.E. (1990). Gene mapping in the cow. In: Advances in Veterinary Science and Comparative Medicine, Vol. 34, Domestic Animal Cytogenetics, R.A. McFeely, ed. pp. 251–270.
Womack, J.E. (1994). Chromosomal evolution from the perspective of the bovine gene map. Anim. Biotechnol. 5, 123–128.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chowdhary, B.P., Frönicke, L., Gustavsson, I. et al. Comparative analysis of the cattle and human genomes: detection of ZOO-FISH and gene mapping-based chromosomal homologies. Mammalian Genome 7, 297–302 (1996). https://doi.org/10.1007/s003359900086
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s003359900086