Abstract
Our group has developed more than 600 DNA markers to build a map of the canine genome. Of these markers, 125 correspond to genes (anchor loci). Here we report the first six autosomal genes assigned to canine chromosomes by fluorescence in situ hybridization (FISH), using cosmid DNA: adenine phosphoribosyl transferase on Chromosome (Chr) 3; creatine kinase muscle type on Chr 4; pyruvate kinase liver and red blood cell type on Chr 2; and colony-stimulating factor-1 receptor, glucose transporter protein-2, and tumor protein p53 on Chr 5. These assignments are based on the karytotype proposed by Stone and associates (Genome 34, 407, 1991) using high-resolution techniques. In addition, we have assigned the Menkes gene to the X Chr of the dog.
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Fratini, A., Simmers, R.N., Callen, D.F., Hyland, V.J., Tischfield, J.A., Stambrook, P.J., Sutherland, G.R. (1986). A new location for the human adenine phosphoribosyltransferase gene (APRT) distal to the haptoglobin (HP) and fra (16) (q23) (FRA16D) loci. Cytogenet. Cell Genet. 43, 10–13.
Fukumoto, H., Seino, S., Imura, H., Seino, Y., Eddy, R.L., Fukushima, Y., Byers, M.G., Shows, T.B., Bell, G.I. (1988). Sequence, tissue distribution, and chromosomal localization of mRNA encoding a human glucose transporter-like protein. Proc. Natl. Acad. Sci. USA 85, 5434–5438.
Isobe, M., Emanuel, B.S. Givol, D., Oren, M., Croce, C.M. (1986). Localization of gene for human p53 tumour antigen to band 17pl3. Nature 320, 84–85.
Le Beau, M.M., Westbrook, C.A., Diaz, M.O., Larson, R.A., Rowley, J.D., Gasson, J.C., Golde, D.W., Scherr, C.J. (1986). Evidence for the involvement of GM-CSF and FMS in the deletion (5q) in myeloid disorders. Science 231, 984–987.
Mouse Genome Database (MGD) (July, 1995), Mouse Genome Informatics Project, The Jackson Laboratory, Bar Harbor, Maine. World Wide Web (URL: http://www.informatics.jax.org).
Nigro, J.M., Schweinfest, C.W., Rajkovic, A., Pavlovic, J., Jamal, S., Dottin, R.P., Hart, J.T., Kamarck, M.E., Rae, P.M.M., Carty, M.D., Martin-DeLeon, P. (1987). cDNA cloning and mapping of the human creatine kinase M gene to 19ql3. Am. J. Hum. Genet. 40, 115–125.
Sambrook, J., Fristch, E.F., Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, 2nd ed. (Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press).
Satoh, H., Tani, K., Yoshida, M.C., Sasaki, M., Miwa, S., Fuji, H. (1988). The human liver-type pyruvate kinase (PKL) gene is on chromosome 1 at band q21. Cytogenet. Cell Genet. 47, 132–133.
Seiden, J.R., Moorhead, P.S., Oehlert, M.L., Patterson, D.F. (1975). The Giemsa banding pattern of the canine karyotype. Cytogenet. Cell Genet. 15, 380–387.
Silver, L.M. (1993). Master locus list (Encyclopedia of the Mouse Genome III) Mamm. Genome 4 (Suppl.), S2-S9.
Stone, D.M., Jacky, P.B., Prieur, D.J. (1991). The giemsa banding pattern of canine chromosomes, using a cell synchronization technique. Genome 34, 407–412.
Verga, V., Hall, B.K., Wang, S., Johnson, S., Higgins, J.V., Glover, T.W. (1991). Localization of the translocation breakpoint in a female with Menkes syndrome to Xql3.2-ql3.3 proximal to PGK-1. Am. J. Hum. Genet. 48, 1133–1138.
Yu, J., Tong, S., Yang-Feng, T., Kao, F-T. (1992). Construction and characterization of a region-specific microdissection library from human chromosome 2q35-q37. Genomics 14, 769–774.
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Guevara-Fujita, M.L., Loechel, R., Venta, P.J. et al. Chromosomal assignment of seven genes on canine chromosomes by fluorescence in situ hybridization. Mammalian Genome 7, 268–270 (1996). https://doi.org/10.1007/s003359900080
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DOI: https://doi.org/10.1007/s003359900080