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A genetic map of Peromyscus with chromosomal assignment of linkage groups (a Peromyscus genetic map)

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Abstract

The rodent genus Peromyscus is the most numerous and species-rich mammalian group in North America. The naturally occurring diversity within this genus allows opportunities to investigate the genetic basis of adaptation, monogamy, behavioral and physiological phenotypes, growth control, genomic imprinting, and disease processes. Increased genomic resources including a high quality genetic map are needed to capitalize on these opportunities. We produced interspecific hybrids between the prairie deer mouse (P. maniculatus bairdii) and the oldfield mouse (P. polionotus) and scored meiotic recombination events in backcross progeny. A genetic map was constructed by genotyping of backcross progeny at 185 gene-based and 155 microsatellite markers representing all autosomes and the X-chromosome. Comparison of the constructed genetic map with the molecular maps of Mus and Rattus and consideration of previous results from interspecific reciprocal whole chromosome painting allowed most linkage groups to be unambiguously assigned to specific Peromyscus chromosomes. Based on genomic comparisons, this Peromyscus genetic map covers ~83 % of the Rattus genome and 79 % of the Mus genome. This map supports previous results that the Peromyscus genome is more similar to Rattus than Mus. For example, coverage of the 20 Rattus autosomes and the X-chromosome is accomplished with only 28 segments of the Peromyscus map, but coverage of the 19 Mus autosomes and the X-chromosome requires 40 chromosomal segments of the Peromyscus map. Furthermore, a single Peromyscus linkage group corresponds to about 91 % of the rat and only 76 % of the mouse X-chromosomes.

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Acknowledgments

This work was supported primarily by NIH GM069601 (MJD, TG), and also by NIH P40 OD010961 (MRF, GS) and NSF 0444165 (MRF, GS). We thank the Colony Manager of the Peromyscus Genetic Stock Center, Janet Crossland, for invaluable help in breeding and record keeping of the animals. We thank M. Peters, T. Tuberville, S. Lance, K. Jones, and A. McKee for assistance in genotyping of microsatellite loci.

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Author notes

  1. Jane Kenney-Hunt & Julie L. Glenn

    Present address: Department of Biology and Environmental Science, Westminster College, Fulton, MO, 65251, USA

  2. Travis C. Glenn

    Present address: Department of Environmental Health Science, University of Georgia, Athens, GA, 30602-7396, USA

  3. Olga V. Tsyusko

    Present address: Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY, 40546-0091, USA

  4. Clifton M. Ramsdell

    Present address: Gene Expression Division, Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules, CA, 94547, USA

  5. Judy Brown

    Present address: Department of Allied Health Sciences, University of Connecticut, Storrs, CT, 06269, USA

Authors and Affiliations

  1. Department of Biological Sciences and Peromyscus Genetic Stock Center, University of South Carolina, Columbia, SC, 29208, USA

    Jane Kenney-Hunt, Adrienne Lewandowski, Julie L. Glenn, Clifton M. Ramsdell, Kimberly R. Shorter, Michael J. Dewey, Gabor Szalai, Paul B. Vrana & Michael R. Felder

  2. Savannah River Ecology Laboratory, University of Georgia, Aiken, SC, 29801, USA

    Travis C. Glenn, Julie L. Glenn & Olga V. Tsyusko

  3. Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT, 06269-2131, USA

    Rachel J. O’Neill & Judy Brown

  4. Department of Biological Chemistry, University of California, Irvine, CA, 92799, USA

    Quang Nguyen & Tony Phan

  5. Department of Environmental Health Science, University of Georgia, Athens, GA, 30602-7396, USA

    Julie L. Glenn

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  1. Jane Kenney-Hunt
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  14. Paul B. Vrana
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Correspondence to Paul B. Vrana or Michael R. Felder.

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Kenney-Hunt, J., Lewandowski, A., Glenn, T.C. et al. A genetic map of Peromyscus with chromosomal assignment of linkage groups (a Peromyscus genetic map). Mamm Genome 25, 160–179 (2014). https://doi.org/10.1007/s00335-014-9500-8

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