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Comparative studies of skeletal muscle proteome and transcriptome profilings between pig breeds

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Abstract

Two genetically different pig breeds, the Korean native pig (KNP) and the Western meat-producing Landrace, show breed-specific traits in stress responsiveness (stress hormone levels), growth performance (live weight), and meat quality (intramuscular fat content). We analyzed expression levels within the proteome and transcriptome of the longissimus muscles of both breeds using two-dimensional electrophoresis (2-DE) and microarray analysis. We constructed a porcine proteome database focused mainly on mitochondrial proteins. In total, 101 proteins were identified, of which approximately 60% were metabolic enzymes and mitochondrial proteins. We screened several proteins and genes related to stress and metabolism in skeletal muscles using comparative analysis. In particular, three stress-related genes (heat shock protein β-1, stress-70 protein, and heat shock 70 kDa protein) were more highly expressed in the Landrace than in the KNP breed. Six metabolism-related genes (peroxisome proliferative activated receptor α, short-chain acyl-CoA dehydrogenase, succinate dehydrogenase, NADH-ubiquinone oxidoreductase, glycerol-3-phosphate dehydrogenase, and sterol regulatory element binding protein-1c), all of which are involved in energy and lipid metabolism, were more highly expressed at the protein or mRNA level in the KNP breed. These data may reflect the breed dependence of traits such as stress responsiveness, growth performance, and meat quality.

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Acknowledgments

This work was supported by a grant (Code 200901FHT010204407) from the BioGreen 21 Program, Rural Development Administration, and partly by Energy through the Bio-Food & Drug Research Center at Konkuk University, Republic of Korea.

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Authors and Affiliations

  1. Department of Applied Biochemistry, College of Biomedical & Health Science, Konkuk University, 322 Danwol-dong, Chungju, 380-701, Republic of Korea

    Nam-Kuk Kim, Hye-Ran Park, Eun-Suk Son, Yeun-Song Kim, Se-Ra Kim & Chang-Soo Lee

  2. National Institutes of Animal Science, Rural Development Administration, Suwon, 441-706, Republic of Korea

    Nam-Kuk Kim, Hwi-Cheul Lee & Duhak Yoon

  3. Department Animal Science, College of Natural Science, Konkuk University, Chungju, 380-701, Republic of Korea

    Oun-Hyun Kim

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  1. Nam-Kuk Kim
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  9. Chang-Soo Lee
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Correspondence to Chang-Soo Lee.

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Kim, NK., Park, HR., Lee, HC. et al. Comparative studies of skeletal muscle proteome and transcriptome profilings between pig breeds. Mamm Genome 21, 307–319 (2010). https://doi.org/10.1007/s00335-010-9264-8

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  • DOI: https://doi.org/10.1007/s00335-010-9264-8

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