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Quantitative trait loci for porcine baseline erythroid traits at three growth ages in a White Duroc × Erhualian F2 resource population

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Abstract

Baseline erythroid indices are increasingly involved as risk factors for common complex diseases in humans. However, little is known about the genetic architecture of baseline erythroid traits in pigs. In this study, hematocrit (Hct), hemoglobin (Hgb), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular volume (MCV), red blood cell (RBC), and red cell distribution width (RDW) were measured in 1420 (day 18), 1410 (day 46), and 1033 (day 240) F2 pigs from a White Duroc × Erhualian intercross resource population. The entire resource population was genotyped for 183 microsatellite loci across the pig genome, and the quantitative trait loci (QTL) analysis was performed for all erythroid-related traits measured with QTL Express based on a least-squares method. A total of 101 QTL, including 46 genome-wide significant QTL and 55 chromosome-wide significant QTL, regulating erythroid traits were found on all pig chromosomes (SSC) except for SSC15 and SSC18. The genome-wide significant QTL were mainly localized on SSC1, 7, 8, 10, and X. These results confirmed most of QTL previously identified in the swine. More importantly, this study detected age-specific QTL for baseline erythroid traits in pigs for the first time. Notably, the QTL for MCV and MCH on day 18 on SSC8 with small intervals of 3 and 4 cM, respectively, provided a good starting point for identifying causal genes underlying MCV and MCH in the future.

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Acknowledgment

This study was financially supported by the National Natural Science Foundation of China (30425045) and the National 973 Program of China (2006CB102100).

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Authors and Affiliations

  1. Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, Nanchang, 330045, China

    Zhengzhi Zou, Jun Ren, Xueming Yan, Xiang Huang, Shujin Yang, Zhiyan Zhang, Bin Yang, Wanbo Li & Lusheng Huang

Authors
  1. Zhengzhi Zou
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  2. Jun Ren
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  3. Xueming Yan
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  4. Xiang Huang
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  5. Shujin Yang
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  6. Zhiyan Zhang
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  7. Bin Yang
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  8. Wanbo Li
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  9. Lusheng Huang
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Corresponding author

Correspondence to Lusheng Huang.

Additional information

Z. Zou and J. Ren contributed equally to this work.

Electronic supplementary material

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335_2008_9142_MOESM1_ESM.doc

MOESM1 [Statistic F-values indicating QTL for erythroid trait at days 18 (a) and 46 (b) on SSC7, and at days 18 (c) and 46 (d) on SSC8. Markers and distance in cM are given on the x-axis, and F-ratios are indicated on the left y-axis. The 1% (…) and 5% (-----) genome-wide significant levels are indicated by two horizontal lines. Hct, hematocrit; Hgb, hemoglobin; MCH, mean corpuscular hemoglobin; MCHC, mean corpuscular hemoglobin content; MCV, mean corpuscular volume; RBC, red blood cell count; RDW, red cell distribution width.] (DOC 87 kb)

335_2008_9142_MOESM2_ESM.doc

MOESM2 [Suggestive quantitative trait loci for baseline erythroid traits at three growth ages in the White Duroc × Erhualian intercross F2 population1] (DOC 124 kb)

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Zou, Z., Ren, J., Yan, X. et al. Quantitative trait loci for porcine baseline erythroid traits at three growth ages in a White Duroc × Erhualian F2 resource population. Mamm Genome 19, 640–646 (2008). https://doi.org/10.1007/s00335-008-9142-9

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  • DOI: https://doi.org/10.1007/s00335-008-9142-9

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