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Defining the expression pattern of the LGI1 gene in BAC transgenic mice

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Abstract

The LGI1 gene has been implicated in the development of epilepsy and the invasion phenotype of glial cells. Controversy over the specific tissue expression pattern of this gene has stemmed from conflicting reports generated using immunohistochemistry and the polymerase chain reaction. LGI1 is one of a four-member family of secreted proteins with high homology and here we demonstrate, using GFP-tagged constructs from the four LGI1family members, that commonly used antibodies against LGI1 cross-react with different family members. With the uncertainty surrounding the use of commercially available antibodies to truly establish the expression pattern of LGI1, we generated transgenic mice carrying the LGI1-containing BAC, RP23-127G7, which had been modified to express the GFP reporter gene under the control of the endogenous regulatory elements required for LGI1 expression. Three founder mice were generated, and immunohistochemistry was used to determine the tissue-specific pattern of expression. In the brain, distinct regions of glial and neuronal cell expression were identified, as well as the choriod plexus, which is largely pia-derived. In addition, strong expression levels were identified in glandular regions of the prostate, individual tubules in the kidney, sympathetic ganglia in the kidney, sebaceous glands in the skin, the islets of Langerhans, the endometrium, and the ovary and testes. All other major organs analyzed were negative. The pattern of reporter gene expression was identical in three individual founder mice, arguing against a position effect altering expression profile due to the integration site of the BAC.

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Acknowledgments

This work was supported by a National Cancer Institute, RPCI Cancer Center Support Grant CA 16056 and a National Institute of Neurological Disorders and Stroke grant NS046706 from the National Institutes of Health.

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Authors and Affiliations

  1. Department of Cancer Genetics, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, New York, 14263, USA

    Karen Head, Sheldon Joseph, Tania Burkhardt, Jeffrey LaDuca, Sei-Ichi Matsui & John K. Cowell

  2. GENSAT Project, The Rockefeller University, New York, New York, 10021, USA

    Shiaoching Gong, Cuidong Wang & Nathaniel Heintz

  3. Department of Genetics, Yale University School of Medicine, New Haven, CT, 06520, USA

    Michael R. Rossi

  4. Department of Pathology, Roswell Park Cancer Institute, Buffalo, New York, 14263, USA

    Mary Vaughan & David G. Hicks

  5. Howard Hughes Medical Institute, The Rockefeller University, New York, New York, 10021, USA

    Nathaniel Heintz

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  1. Karen Head
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  2. Shiaoching Gong
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  3. Sheldon Joseph
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  4. Cuidong Wang
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  5. Tania Burkhardt
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  6. Michael R. Rossi
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  7. Jeffrey LaDuca
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  8. Sei-Ichi Matsui
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  9. Mary Vaughan
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  10. David G. Hicks
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  11. Nathaniel Heintz
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  12. John K. Cowell
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Correspondence to John K. Cowell.

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Head, K., Gong, S., Joseph, S. et al. Defining the expression pattern of the LGI1 gene in BAC transgenic mice. Mamm Genome 18, 328–337 (2007). https://doi.org/10.1007/s00335-007-9024-6

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  • DOI: https://doi.org/10.1007/s00335-007-9024-6

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