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Linkage analysis with an interbreed backcross maps Dalmatian hyperuricosuria to CFA03

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Abstract

Dalmatians, like humans, excrete uric acid in their urine. All other dogs and most mammals excrete allantoin, a water-soluble compound that is further along the purine degradation pathway. Excretion of uric acid at high concentrations (hyperuricosuria) predisposes Dalmatians to the formation of urinary urate calculi. Hyperuricosuria (huu) is found in all Dalmatians tested and is inherited as an autosomal recessive trait. A genome scan and linkage analysis performed on a Dalmatian × Pointer interbreed backcross detected a single linked marker, REN153P03, located on CFA03. Haplotype analysis of the region around this marker defined a 3.3-Mb interval flanked by single recombination events. This interval, which contains the huu mutation, is estimated to include 24 genes.

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Acknowledgments

The authors thank Aaron Wong for his help with calculating the genome scan LOD scores, and the canine research group at the UC Davis Veterinary Genetics Laboratory for providing the multiplexed genotyping panels.

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Authors and Affiliations

  1. Department of Population Health and Reproduction School of Veterinary Medicine, University of California, 1 Shields Avenue, Davis, California, 95616, USA

    Noa Safra & Danika L. Bannasch

  2. Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Purdue University, West Lafayette, Indiana, 47907, USA

    Robert H. Schaible

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  1. Noa Safra
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  2. Robert H. Schaible
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  3. Danika L. Bannasch
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Correspondence to Danika L. Bannasch.

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Safra, N., Schaible, R.H. & Bannasch, D.L. Linkage analysis with an interbreed backcross maps Dalmatian hyperuricosuria to CFA03. Mamm Genome 17, 340–345 (2006). https://doi.org/10.1007/s00335-005-0137-5

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  • DOI: https://doi.org/10.1007/s00335-005-0137-5

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