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Genetic characterization of a new set of recombinant inbred lines (LGXSM) formed from the intercross of SM/J and LG/J inbred mouse strains

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Abstract

A new set of LGXSM recombinant inbred (RI) strains is presented. The RI strain panel consists of 18 remaining strains of the original 55 founding strains. Strain characterization is based on 506 polymorphic microsatellites and 4289 single nucleotide polymorphisms (SNPs) distributed across the genome. Average microsatellite intermarker distance is 4.80 ± 4.84 Mb or 2.91 ± 3.21 F2 cM. SNPs are more densely spaced at 0.57 ± 1.27 Mb. Ninety-five percent of all microsatellite intermarker intervals are separated by less than 15.00 Mb or 8.50 F2 cM, while 95% of the SNPs are less than 0.95 Mb apart. Strains show expected low levels of nonsyntenic association among loci and complete genomic independence. During inbreeding, the RI strains went through strong natural selection on the agouti locus on Chromosome 2, especially when the epistatically interacting tyrosinase locus on Chromosome 7 carried the wild-type allele. The LG/J and SM/J strains differ in a large number of biomedically important traits, and they and their intercross progeny have been used in multiple mapping studies. The LG×SM RI strain panel provides a powerful new resource for mapping the genetic bases of complex traits and should prove to be of great biomedical utility in modeling complex human diseases such as obesity and diabetes.

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Acknowledgments

The authors thank all those who helped with the creation and maintenance of the RI mouse lines, and who over the years have worked with and cared for the mice. They also thank T. H. Ehrich and J. P. Kenney-Hunt for insightful comments and critical discussion. This study was supported by NIH grants RR015116 and DK055736 (to JMC).

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Authors and Affiliations

  1. Department of Anatomy and Neurobiology, Washington University School of Medicine, 660 S. Euclid Ave., St. Louis, Missouri, 63110, USA

    Tomas Hrbek, Reinaldo Alves de Brito, B. Wang, L. Susan Pletscher & James M. Cheverud

  2. Departamento de Biologia Geral – ICB, Universidade Federal de São Carlos, São Carlos, São Paulo, Brazil

    Reinaldo Alves de Brito

Authors
  1. Tomas Hrbek
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  2. Reinaldo Alves de Brito
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  3. B. Wang
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  4. L. Susan Pletscher
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  5. James M. Cheverud
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Corresponding author

Correspondence to James M. Cheverud.

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Appendices

Appendix 1

This appendix is a table with all microsatellite and SNP markers and their states for the 18 RI strains. It is available at http://www.thalamus.wustl.edu/cheverudlab/

Appendix 2

This appendix shows the analysis of heterozygosity in all 18 RI strains across all 19 autosomes and the X sex chromosome. It is available at http://www.thalamus.wustl.edu/cheverudlab/

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Hrbek, T., de Brito, R.A., Wang, B. et al. Genetic characterization of a new set of recombinant inbred lines (LGXSM) formed from the intercross of SM/J and LG/J inbred mouse strains. Mamm Genome 17, 417–429 (2006). https://doi.org/10.1007/s00335-005-0038-7

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  • DOI: https://doi.org/10.1007/s00335-005-0038-7

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