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Quantitative trait loci in Chromosomes 3, 8, and 9 regulate antibody production against Salmonella flagellar antigensin the mouse

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Abstract

Two mouse lines were produced by bidirectional selection according to the high (HIII) or low (LIII) antibody responsiveness against Salmonella flagellar antigens (Selection III). In the present work we conducted a genomewide scan to map the quantitative trait loci (QTL) involved in the antibody response regulation in these selected mice. HIII and LIII genomes were screened with microsatellite markers and those found polymorphic between the lines (146) were used for linkage analysis in F2 (HIII × LIII) intercross. Simple interval mapping analysis was performed using Mapmanager QTX software. Three highly significant QTL linked to antibody production against Salmonella flagellar antigens have been demonstrated in Chromosomes 3, 8, and 9. HIII and LIII lines differ in the resistance to several diseases, therefore, the relevance of these QTL with the genetic factors involved in infections, autoimmunity, and neoplastic disease progression is discussed.

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References

  • G Biozzi D Mouton OA Sant’Anna HC Passos M Gennari et al. (1979) ArticleTitleGenetics of immunoresponsiveness to natural antigens in the mouse Curr Top Microbiol Immunol 85 31–98 Occurrence Handle1:STN:280:Bi%2BD38zmtFE%3D Occurrence Handle90582

    CAS  PubMed  Google Scholar 

  • V Covelli D Mouton V Di Majo Y Bouthillier C Bangrazi et al. (1989) ArticleTitleInheritance of immune responsiveness, life span, and disease incidence in interline crosses of mice selected for high or low multispecific antibody production J Immunol 142 1224–1234 Occurrence Handle1:STN:280:BiaC38vjs1c%3D Occurrence Handle2915118

    CAS  PubMed  Google Scholar 

  • A Darvasi (1997) ArticleTitleThe effect of selective genotyping on QTL mapping accuracy Mamm Genome 8 67–68 Occurrence Handle10.1007/s003359900353 Occurrence Handle1:CAS:528:DyaK2sXnsVOntg%3D%3D Occurrence Handle9021156

    Article  CAS  PubMed  Google Scholar 

  • F Benedetti ParticleDe P Pignatti M Vivarelli C Meazza G Ciliberto et al. (2001) ArticleTitleIn vivo neutralization of human IL-6 (hIL-6) achieved by immunization of hIL-6-transgenic mice with a hIL-6 receptor antagonist J Immunol 16 4334–4340

    Google Scholar 

  • M Franco ParticleDe MF Gille–Perramant JC Mevel J Couderc (1995) ArticleTitleT helper subset involvement in two high antibody responder lines of mice (Biozzi mice): HI (susceptible) and HII (resistant) to collagen-induced arthritis Eur J Immunol 25 132–136 Occurrence Handle7843223

    PubMed  Google Scholar 

  • V Ferreira M Gennari MH Reis M Siqueira C Stiffel et al. (1985) ArticleTitlePotentialities of immunocompetent cells in high and low antibody-producing lines of mice obtained by selective breedings for responsiveness to flagellar or somatic antigens of Salmonellae J Immunogenet 12 309–319 Occurrence Handle1:STN:280:BimB2sbntVA%3D Occurrence Handle3837055

    CAS  PubMed  Google Scholar 

  • OM Ibanez D Mouton OG Ribeiro Y Bouthillier M Franco ParticleDe et al. (1999) ArticleTitleLow antibody responsiveness is found to be associated with resistance to chemical skin tumorigenesis in several lines of Biozzi mice Cancer Lett 136 153–158 Occurrence Handle10.1016/S0304-3835(98)00317-6 Occurrence Handle1:STN:280:DyaK1M3otFyktw%3D%3D Occurrence Handle10355744

    Article  CAS  PubMed  Google Scholar 

  • E Lander L Kruglyak (1995) ArticleTitleGenetic dissection of complex traits: guidelines for interpreting and reporting linkage results Nat Genet 11 241–247 Occurrence Handle1:CAS:528:DyaK2MXptlSjs74%3D Occurrence Handle7581446

    CAS  PubMed  Google Scholar 

  • KF Manly RH Cudmore SuffixJr JM Meer (2001) ArticleTitleRoswell Park Cancer Institute, Map Manager QTX, cross-platform software for genetic mapping Mamm Genome 12 930–932 Occurrence Handle10.1007/s00335-001-1016-3 Occurrence Handle1:CAS:528:DC%2BD3MXosFGgtbo%3D Occurrence Handle11707780

    Article  CAS  PubMed  Google Scholar 

  • X Montagutelli T Serikawa JL Guenet (1991) ArticleTitlePCR-analyzed microsatellites: data concerning laboratory and wild-derived mouse inbred strains Mamm Genome 1 255–259 Occurrence Handle1:CAS:528:DyaK38Xks1eqs7w%3D Occurrence Handle1794054

    CAS  PubMed  Google Scholar 

  • D Mouton C Stiffel G Biozzi (1985) ArticleTitleGenetic factors of immunity against infection Ann Inst Pasteur Immunol 136D 131–141 Occurrence Handle1:STN:280:BimC3c7ovVA%3D Occurrence Handle3936395

    CAS  PubMed  Google Scholar 

  • H Nagase JH Mao A Balmain (1999) ArticleTitleA subset of skin tumor modifier loci determines survival time of tumor-bearing mice Proc Natl Acad Sci USA 96 15032–15037 Occurrence Handle10.1073/pnas.96.26.15032 Occurrence Handle1:CAS:528:DC%2BD3cXhtFaksQ%3D%3D Occurrence Handle10611333

    Article  CAS  PubMed  Google Scholar 

  • JM Otto G Cs-Szabo J Gallagher S Velins K Mikecz et al. (1999) ArticleTitleIdentification of multiple loci linked to inflammation and autoantibody production by a genome scan of a murine model of rheumatoid arthritis Arthritis Rheum 42 2524–2531 Occurrence Handle10.1002/1529-0131(199912)42:12<2524::AID-ANR4>3.0.CO;2-0 Occurrence Handle1:CAS:528:DC%2BD3cXjslagtw%3D%3D Occurrence Handle10615997

    Article  CAS  PubMed  Google Scholar 

  • A Puel PC Groot MG Lathrop P Demant D Mouton (1995) ArticleTitleMapping of genes controlling quantitative antibody production in Biozzi mice J Immunol 154 5799–5805 Occurrence Handle1:CAS:528:DyaK2MXlvVWgtL4%3D Occurrence Handle7751629

    CAS  PubMed  Google Scholar 

  • A Puel JC Mevel Y Bouthillier N Feingold WH Fridman et al. (1996) ArticleTitleToward genetic dissection of high and low antibody responsiveness in Biozzi mice Proc Natl Acad Sci USA 93 14742–14746 Occurrence Handle10.1073/pnas.93.25.14742 Occurrence Handle1:CAS:528:DyaK28XnsVOjsbo%3D Occurrence Handle8962125

    Article  CAS  PubMed  Google Scholar 

  • A Puel JC Mevel Y Bouthillier C Decreusefond WH Fridman et al. (1998) ArticleTitleIdentification of two quantitative trait loci involved in antibody production on mouse chromosome 8 Immunogenetics 47 326–331 Occurrence Handle10.1007/s002510050365 Occurrence Handle1:CAS:528:DyaK1cXhtVOktLc%3D Occurrence Handle9472069

    Article  CAS  PubMed  Google Scholar 

  • MH Reis OM Ibanez WH Cabrera OG Ribeiro D Mouton et al. (1992) ArticleTitleT-helper functions in lines of mice selected for high or low antibody production (Selection III): modulation by anti-CD4+ monoclonal antibody Immunology 75 80–85 Occurrence Handle1:CAS:528:DyaK38Xht1Knu7c%3D Occurrence Handle1371495

    CAS  PubMed  Google Scholar 

  • OA Sant’Anna VC Ferreira MH Reis M Gennari OM Ibanez et al. (1982) ArticleTitleGenetic parameters of the polygenic regulation of antibody responsiveness to flagellar and somatic antigens of salmonellae J Immunogenet 9 191–205 Occurrence Handle1:STN:280:Bi2B28jitlA%3D Occurrence Handle6809839

    CAS  PubMed  Google Scholar 

  • OA Sant’Anna S Massa D Mouton Y Bouthillier JC Mevel et al. (1989) ArticleTitleSalmonella typhimurium infection in high and low antibody responder mice: inverse correlation between antibody responsiveness and resistance to infection FEMS Microbiol Immunol 1 465–471 Occurrence Handle1:STN:280:By%2BC1c7gtVU%3D Occurrence Handle2697320

    CAS  PubMed  Google Scholar 

  • OA Sant’Anna Y Bouthillier JC Mevel M Franco ParticleDe D Mouton (1991) ArticleTitleIsotypic distribution of antibody responses in lines of mice selected for high or low immunoresponsiveness Braz J Med Biol Res 24 407–416 Occurrence Handle1:STN:280:By2A2s%2FgsVY%3D Occurrence Handle1823254

    CAS  PubMed  Google Scholar 

  • G Sebastiani L Olien S Gauthier E Skamene K Morgan et al. (1998) ArticleTitleMapping of genetic modulators of natural resistance to infection with Salmonella typhimurium in wild-derived mice Genomics 47 180–186 Occurrence Handle10.1006/geno.1997.5116 Occurrence Handle1:CAS:528:DyaK1cXhtVCnsbY%3D Occurrence Handle9479490

    Article  CAS  PubMed  Google Scholar 

  • M Siqueira A Bandieri MS Reis OA Sant’Anna G Biozzi (1976) ArticleTitleSelective breeding of mice for antibody responsiveness to flagellar and somatic antigens of salmonellae Eur J Immunol 6 241–249 Occurrence Handle1:STN:280:CSiD2cfltlI%3D Occurrence Handle791654

    CAS  PubMed  Google Scholar 

  • T Takai (2002) ArticleTitleRoles of Fc receptors in autoimmunity Nat Rev Immunol 2 580–592 Occurrence Handle1:CAS:528:DC%2BD38XlslGru7Y%3D Occurrence Handle12154377

    CAS  PubMed  Google Scholar 

  • AG Trezena CM Souza A Borrego S Massa M Siqueira et al. (2002) ArticleTitleCo-localization of quantitative trait loci regulating resistance to Salmonella typhimurium infection and specific antibody production phenotypes Microbes Infect 4 1409–1415 Occurrence Handle10.1016/S1286-4579(02)00023-0 Occurrence Handle1:CAS:528:DC%2BD38XptlWltL0%3D Occurrence Handle12475631

    Article  CAS  PubMed  Google Scholar 

  • J Snick ParticleVan (1990) ArticleTitleInterleukin-6: an overview Annu Rev Immunol 8 253–278 Occurrence Handle10.1146/annurev.iy.08.040190.001345 Occurrence Handle2188664

    Article  PubMed  Google Scholar 

  • SM Vidal D Malo K Vogan E Skamene P Gros (1993) ArticleTitleNatural resistance to infection with intracellular parasites: isolation of a candidate for Bcg Cell 73 469–485 Occurrence Handle10.1016/0092-8674(93)90135-D Occurrence Handle1:CAS:528:DyaK3sXltFShurc%3D Occurrence Handle8490962

    Article  CAS  PubMed  Google Scholar 

  • EK Wakeland K Liu RR Graham TW Behrens (2001) ArticleTitleDelineating the genetic basis of systemic lupus erythematosus Immunity 15 397–408 Occurrence Handle10.1016/S1074-7613(01)00201-1 Occurrence Handle1:CAS:528:DC%2BD3MXntlSntLk%3D Occurrence Handle11567630

    Article  CAS  PubMed  Google Scholar 

  • JJ Weis BA McCracken Y Ma D Fairbairn RJ Roper et al. (1999) ArticleTitleIdentification of quantitative trait loci governing arthritis severity and humoral responses in the murine model of Lyme disease J Immunol 162 948–956 Occurrence Handle1:CAS:528:DyaK1MXovFOqug%3D%3D Occurrence Handle9916719

    CAS  PubMed  Google Scholar 

  • J Wu JA Longmate G Adamus PA Hargrave EK Wakeland (1996) ArticleTitleInterval mapping of quantitative trait loci controlling humoral immunity to exogenous antigens: evidence that non-MHC immune response genes may also influence susceptibility to autoimmunity J Immunol 157 2498–2505 Occurrence Handle1:CAS:528:DyaK28Xls1aisLc%3D Occurrence Handle8805650

    CAS  PubMed  Google Scholar 

  • HT Yang J Jirholt L Svensson M Sundvall L Jansson et al. (1999) ArticleTitleIdentification of genes controlling collagen-induced arthritis in mice: striking homology with susceptibility loci previously identified in the rat J Immunol 163 2916–2921 Occurrence Handle1:CAS:528:DyaK1MXls1OisLk%3D Occurrence Handle10453039

    CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We would like to thank Dr. Edward Wakeland and Dr. Denise Mouton for their helpful suggestions on this manuscript. This work was funded by grants from the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) and the Conselho Nacional de Pesquisas (CNPq).

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Authors and Affiliations

  1. Laboratório de Imunogenética, Instituto Butantan, Av. Vital Brazil, 1500, São Paulo, SP, 05503900, Brazil

    Carla M. de Souza, Wafa H.K. Cabrera, Nancy Starobinas, Orlando G. Ribeiro, Maria Siqueira, Olga M. Ibañez & Marcelo De Franco

  2. Department of Pathology, University of Florida, Gainesville, Florida, FL 32611, USA

    Laurence Morel

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  1. Carla M. de Souza
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  2. Laurence Morel
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  3. Wafa H.K. Cabrera
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  8. Marcelo De Franco
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Correspondence to Marcelo De Franco.

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de Souza, C.M., Morel, L., Cabrera, W.H. et al. Quantitative trait loci in Chromosomes 3, 8, and 9 regulate antibody production against Salmonella flagellar antigensin the mouse. Mamm Genome 15, 630–636 (2004). https://doi.org/10.1007/s00335-004-2378-0

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  • DOI: https://doi.org/10.1007/s00335-004-2378-0

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