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A high-resolution comparative map of canine Chromosome 5q14.3–q33 constructed utilizing the 1.5× canine genomesequence

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Abstract

A high-density map of the region of canine Chromosome 5 (CFA5) surrounding the evolutionary breakpoint between human Chromosomes 1p32 and 17p11 was constructed by integrating a radiation hybrid map including 41 microsatellites, 10 BACs, and 59 genes and a linkage map including 18 markers. A collection of canine genomic survey sequences providing 1.5× coverage was used to identify dog orthologs of human genes, proving instrumental in the development of this map. Of particular interest is the canine BHD gene, within which we have previously described a single nucleotide polymorphism associated with Hereditary Multifocal Renal Cystadenocarcinoma and Nodular Dermatofibrosis (RCND) in German Shepherd dogs. The corresponding region of the human genome is particularly gene rich, containing genes involved in development, metabolism, and cancer that are likely to be of interest in future mapping studies. This current mapping effort on CFA5 expands the degree to which initial findings of linkage in canine families can be followed by successful positional cloning efforts and increases the value of the human genome sequence for defining candidate genes. Moreover, this study demonstrates the utility of genomic survey sequences when combined with accurate genome maps for rapid mapping of disease susceptibility loci.

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Acknowledgments

This work was supported by US Army grant BAAD AAD 19-01-0658, a Burroughs Wellcome Award for Innovative Genomics, an NCI K05 award (K05 CA 90574), an ACS grant (RPG 96-045-04-MGO) and the American Kennel Club Canine Health Foundation to E.A.O and F.G. It was also supported in part by grants to F.L. [Norwegian Research Council (110642/120 and 153544/14)] and M.B. and R.T. (AKC Canine Health Foundation and the PetPlan Charitable Trust).

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Authors and Affiliations

  1. Clinical and Human Biology Divisions, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave., D4-100, P.O. Box 19024, Seattle, Washington, 98109-1024, USA

    Kenine E. Comstock & Elaine A. Ostrander

  2. Norwegian School of Veterinary Science, Oslo, Norway

    Frode Lingaas

  3. The Institute for Genomic Research, Rockville, Maryland, 20850, USA

    Ewen F. Kirkness

  4. Génétique et Développement, Faculté de Médecine, UMR 6061 CNRS, 35043, Rennes Cédex, France

    Christophe Hitte & Francis Galibert

  5. Oncology Research, Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk, CB8 7UU, United Kingdom

    Rachael Thomas

  6. Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, 27606, USA

    Rachael Thomas & Matthew Breen

Authors
  1. Kenine E. Comstock
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  2. Frode Lingaas
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  3. Ewen F. Kirkness
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  4. Christophe Hitte
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  5. Rachael Thomas
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  6. Matthew Breen
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  7. Francis Galibert
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  8. Elaine A. Ostrander
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Corresponding author

Correspondence to Elaine A. Ostrander.

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Comstock, K.E., Lingaas, F., Kirkness, E.F. et al. A high-resolution comparative map of canine Chromosome 5q14.3–q33 constructed utilizing the 1.5× canine genomesequence. Mamm Genome 15, 544–551 (2004). https://doi.org/10.1007/s00335-004-2365-5

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  • DOI: https://doi.org/10.1007/s00335-004-2365-5

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