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Polymorphisms in equine immune response genes and their associations with infections

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Abstract

Polymorphic markers identified in the horse genes encoding the interleukin 12 p40 subunit, interferon γ, tumor necrosis factor receptor 1, and inducible nitric oxide synthase were identified and tested, along with additional markers, for associations with two important horse infections: Rhodococcus equi and Lawsonia intracellularis. Eight immune response-related and 14 microsatellite loci covering 12 out of 31 equine autosomes were used for the association analysis. Markers located on horse Chromosomes Eca10 and 15 were significantly associated with the presence of high numbers of R. equi in transtracheal aspirates. Significant associations of markers located on Eca9, 15, and 21 with fecal shedding of Lawsonia intracellularis were found. Marginal associations with tumor necrosis factor α, interferon γ, and other genes suggested that variations in immune response-related genes could underlie the phenotypic variation observed.

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Acknowledgments

Technical assistance of Mrs. E. Hantáková, Mrs. V. Nováková, and Mrs. J. Hofírková is acknowledged. The work was supported by the Grant Agency of the Czech Republic projects 523/99/1152, 523/02/1526, and 524/00/1275 and by the Ministry of Education of the Czech Republic, project MSM 161700001. The authors are indebted to ing. J. Hlačik for his understanding and cooperation and dr. D.G. Fraser for her helpful comments on the manuscript.

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Authors and Affiliations

  1. Faculty of Veterinary Medicine, Institute of Animal Genetics, Palackého 1/3, 612 42, Brno, Czech Republic

    Petr Horřín, Ján Matiašovic, Mirko Vyskočil, Ludmila Lukeszová, Petr Králík & Leona Krřenková

  2. Faculty of Veterinary Medicine, Institute of Microbiology and Immunology, Brno, Czech Republic

    Jirří Smola & Katarína Tomanová

  3. Laboratory of Immunogenetics, Czech and Moravian Society of Animal Breeders, Hradistko p.M., Czech Republic

    Vladimír Glasnák & Dana Schröffelová

  4. Department of Genetics, Mendel University of Agriculture and Forestry, Brno, Czech Republic

    Aleš Knoll

  5. Faculty of Veterinary Medicine, Equine Clinic, Brno, Czech Republic

    Markéta Sedlinská & Petr Jahn

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  1. Petr Horřín
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Correspondence to Petr Horřín.

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Horřín, P., Smola, J., Matiašovic, J. et al. Polymorphisms in equine immune response genes and their associations with infections. Mamm Genome 15, 843–850 (2004). https://doi.org/10.1007/s00335-004-2356-6

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  • DOI: https://doi.org/10.1007/s00335-004-2356-6

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