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Microcell-mediated chromosome transfer (MMCT): small cells with huge potential

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Abstract

Microcell-mediated chromosome transfer (MMCT) is a technique that has been in use since the 1970s for the fusion of microcells, containing single or a small number of chromosomes, with whole cells, and the subsequent selection of the hybrids. MMCT can be carried out with somatic cells, embryonic carcinoma (EC) or embryonic stem (ES) cell recipients, to study in vitro or in vivo effects of the transferred genetic material. These effects may be unpredictable–do the transferred genes function normally while in the regulatory milieu of the host cell? Will epigenetic effects become apparent, and how will these alter gene expression? What happens to the host cell phenotype? Here, we present a review of MMCT in which we argue that, although this is an old technique, its adaptability and efficiency make it an excellent method for the dissection of gene function and dysfunction in a very wide range of current systems.

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References

  1. C Backsch N Wagenbach M Nonn S Leistritz E Stanbridge et al. (2001) ArticleTitleMicrocell-mediated transfer of chromosome 4 into HeLa cells suppresses telomerase activity. Genes Chromosomes Cancer 31 196–198 Occurrence Handle10.1002/gcc.1134.abs Occurrence Handle1:CAS:528:DC%2BD3MXjvFOnur0%3D Occurrence Handle11319808

    Article  CAS  PubMed  Google Scholar 

  2. A Barrientos S Muller R Dey J Wienberg CT Moraes (2000) ArticleTitleCytochrome c oxidase assembly in primates is sensitive to small evolutionary variations in amino acid sequence. Mol Biol Evol 17 1508–1519 Occurrence Handle1:CAS:528:DC%2BD3cXnt1Cnsbs%3D Occurrence Handle11018157

    CAS  PubMed  Google Scholar 

  3. G Barski S Soriel F Cornefert (1960) ArticleTitleProduction dans des cultures in vitro de deux souches cellulaires en association, de cellules de caractere “hybride”. C R Acad Sci Paris 251 1825–1827 Occurrence Handle1:STN:280:CC%2BB3c7ivVw%3D Occurrence Handle14448491

    CAS  PubMed  Google Scholar 

  4. Q Cao H Abeysinghe O Chow J Xu H Kaung et al. (2001) ArticleTitleSuppression of tumorigenicity in human ovarian carcinoma cell line SKOV-3 by microcell-mediated transfer of chromosome 11. Cancer Genet Cytogenet 129 131–137 Occurrence Handle10.1016/S0165-4608(01)00442-3 Occurrence Handle1:CAS:528:DC%2BD3MXmvVKksbw%3D Occurrence Handle11566343

    Article  CAS  PubMed  Google Scholar 

  5. V Defendi (1969) Heterospecific genome interaction. Heterospecific Genome Interaction. Wistar Inst Symp Monogr 9. Wistar Institute Press Philadelphia

    Google Scholar 

  6. G deJong AH Telenius H Telenius CF Perez JI Drayer et al. (1999) ArticleTitleMammalian artificial chromosome pilot production facility: large-scale isolation of functional satellite DNA-based artificial chromosomes. Cytometry 35 129–133 Occurrence Handle10.1002/(SICI)1097-0320(19990201)35:2<129::AID-CYTO4>3.3.CO;2-1 Occurrence Handle1:STN:280:DC%2BD3c%2FhvFGqtA%3D%3D Occurrence Handle10554168

    Article  CAS  PubMed  Google Scholar 

  7. ES Dieken REK Fournier (1996) ArticleTitleHomologous modification of human chromosomal genes in chicken B-cell × human microcell hybrids. Methods 9 56–63 Occurrence Handle10.1006/meth.1996.0008 Occurrence Handle1:CAS:528:DyaK28XitVahtLk%3D Occurrence Handle9245343

    Article  CAS  PubMed  Google Scholar 

  8. SF Dowdy DJ Scanlon CL Fasching G Casey EJ Stanbridge (1990) ArticleTitleIrradiation microcell-mediated chromosome transfer (XMMCT): the generation of specific chromosomal arm deletions. Genes Chromosomes Cancer 2 318–327 Occurrence Handle1:STN:280:By6D1MfgvVU%3D Occurrence Handle2268580

    CAS  PubMed  Google Scholar 

  9. T Ege NR Ringertz (1974) ArticleTitlePreparation of microcells by enucleation of micronucleate cells. Exp Cell Res 87 378–382 Occurrence Handle1:CAS:528:DyaE2cXls1Ojs78%3D Occurrence Handle4370277

    CAS  PubMed  Google Scholar 

  10. RE Fournier FH Ruddle (1977) ArticleTitleMicrocell-mediated transfer of murine chromosomes into mouse, Chinese hamster, and human somatic cells. Proc Natl Acad Sci USA 74 319–323 Occurrence Handle1:STN:280:CSiC3c3nsVc%3D Occurrence Handle264685

    CAS  PubMed  Google Scholar 

  11. RA Gatti (2001) ArticleTitleThe inherited basis of human radiosensitivity. Acta Oncol 40 702–711 Occurrence Handle10.1080/02841860152619115 Occurrence Handle1:STN:280:DC%2BD38%2FjvVSkuw%3D%3D Occurrence Handle11765064

    Article  CAS  PubMed  Google Scholar 

  12. P Giraldo L Montoliu (2001) ArticleTitleSize matters: use of YACs, BACs and PACs in transgenic animals. Transgenic Res 10 83–103 Occurrence Handle1:CAS:528:DC%2BD3MXis1Kmur0%3D Occurrence Handle11305364

    CAS  PubMed  Google Scholar 

  13. SJ Goss H Harris (1975) ArticleTitleNew method for mapping genes in human chromosomes. Nature 255 680–684 Occurrence Handle1:CAS:528:DyaE2MXlt1ynsbw%3D Occurrence Handle1169690

    CAS  PubMed  Google Scholar 

  14. R Gudi SS Sandhu RS Athwal (1989) ArticleTitleA genetic method to quantitate induced chromosome breaks using a mouse/human monochromosomal hybrid cell line: identification of potential clastogenic agents. Mutat Res 225 149–156 Occurrence Handle10.1016/0165-7992(89)90112-7 Occurrence Handle1:STN:280:BiaC1c7nvVw%3D Occurrence Handle2927436

    Article  CAS  PubMed  Google Scholar 

  15. S Gupta EJ Stanbridge (2001) ArticleTitlePaired human fibrosarcoma cell lines that possess or lack endogenous mutant N-ras alleles as experimental model for Ras signaling pathways. Methods Enzymol 333 290–306 Occurrence Handle1:CAS:528:DC%2BD3MXltV2nu74%3D Occurrence Handle11400345

    CAS  PubMed  Google Scholar 

  16. JJ Harrington G Van Bokkelen RW Mays K Gustashaw HF Willard (1997) ArticleTitleFormation of de novo centromeres and construction of first-generation human artificial microchromosomes. Nat Genet 15 345–355 Occurrence Handle1:CAS:528:DyaK2sXitF2iu7s%3D Occurrence Handle9090378

    CAS  PubMed  Google Scholar 

  17. RD Harrington AP Geballe (1996) ArticleTitleHuman immunodeficiency virus type-1 susceptible whole cell and microcell hybrids. Ann Clin Lab Sci 26 522–530 Occurrence Handle1:CAS:528:DyaK28XmvFWrs78%3D Occurrence Handle8908322

    CAS  PubMed  Google Scholar 

  18. H Harris JF Watkins CE Ford GI Schoefl (1966) ArticleTitleArtificial heterokaryons of animal cells from different species. J Cell Sci 1 1–30 Occurrence Handle1:STN:280:CCmC387gsVU%3D Occurrence Handle4286457

    CAS  PubMed  Google Scholar 

  19. H Hasle (2001) ArticleTitlePattern of malignant disorders in individuals with Down’s syndrome. Lancet Oncol 2 429–436 Occurrence Handle10.1016/S1470-2045(00)00435-6 Occurrence Handle1:STN:280:DC%2BD387ntlGitw%3D%3D Occurrence Handle11905737

    Article  CAS  PubMed  Google Scholar 

  20. R Heller KE Brown C Burgtorf WR Brown (1996) ArticleTitleMini-chromosomes derived from the human Y chromosome by telomere directed chromosome breakage. Proc Natl Acad Sci USA 93 7125–7130 Occurrence Handle10.1073/pnas.93.14.7125 Occurrence Handle1:CAS:528:DyaK28Xkt1Whtbs%3D Occurrence Handle8692956

    Article  CAS  PubMed  Google Scholar 

  21. D Hernandez PJ Mee JE Martin VL Tybulewicz EMC Fisher (1999) ArticleTitleTranschromosomal mouse embryonic stem cell lines and chimeric mice that contain freely segregating segments of human chromosome 21. Hum Mol Genet 8 923–933 Occurrence Handle10.1093/hmg/8.5.923 Occurrence Handle1:CAS:528:DyaK1MXivFamt7w%3D Occurrence Handle10196383

    Article  CAS  PubMed  Google Scholar 

  22. J Inoue K Mitsuya S Maegawa H Kugoh M Kadota et al. (2001) ArticleTitleConstruction of 700 human/mouse A9 monochromosomal hybrids and analysis of imprinted genes on human chromosome 6. J Hum Genet 46 137–145 Occurrence Handle11310581

    PubMed  Google Scholar 

  23. I Ishida K Tomizuka H Yoshida T Tahara N Takahashi et al. (2002) ArticleTitleProduction of human monoclonal and polyclonal antibodies in TransChromo animals. Cloning Stem Cells 4 91–102 Occurrence Handle10.1089/153623002753632084 Occurrence Handle1:CAS:528:DC%2BD38Xjt1Grsr4%3D Occurrence Handle12006160

    Article  CAS  PubMed  Google Scholar 

  24. JE Itzhaki CS Gilbert AC Porter (1997) ArticleTitleConstruction by gene targeting in human cells of a “conditional” CDC2 mutant that rereplicates its DNA. Nat Genet 15 258–265 Occurrence Handle1:CAS:528:DyaK2sXhsVGmtro%3D Occurrence Handle9054937

    CAS  PubMed  Google Scholar 

  25. M Jasin SJ Elledge RW Davis P Berg (1990) ArticleTitleGene targeting at the human CD4 locus by epitope addition. Genes Dev 4 157–166 Occurrence Handle1:CAS:528:DyaK3cXhsFejs7o%3D Occurrence Handle1692556

    CAS  PubMed  Google Scholar 

  26. M Kameyama (2001) ArticleTitleQuantum cellular biology: a curious example of a cat. Med Hypotheses 57 358–360 Occurrence Handle10.1054/mehy.2001.1349 Occurrence Handle1:CAS:528:DC%2BD3MXntVKjtbo%3D Occurrence Handle11516229

    Article  CAS  PubMed  Google Scholar 

  27. Y Kazuki T Shinohara K Tomizuka M Katoh A Ohguma et al. (2001) ArticleTitleGermline transmission of a transferred human chromosome 21 fragment in transchromosomal mice. J Hum Genet 46 600–603 Occurrence Handle10.1007/s100380170028 Occurrence Handle1:CAS:528:DC%2BD3MXns1yqtLs%3D Occurrence Handle11587075

    Article  CAS  PubMed  Google Scholar 

  28. AM Killary RE Fournier (1995) ArticleTitleMicrocell fusion. Methods Enzymol 254 133–152 Occurrence Handle1:CAS:528:DyaK2MXps1Cksb4%3D Occurrence Handle8531682

    CAS  PubMed  Google Scholar 

  29. M Kumata M Shimizu M Oshiura M Uchida T Tsutsui (2002) ArticleTitleInduction of cellular senescence in a telomerase negative human immortal fibroblast cell line, LCS-AF 1-3 by human chromosome 6. Int J Oncol 21 851–856 Occurrence Handle1:CAS:528:DC%2BD38XotFKitbw%3D Occurrence Handle12239626

    CAS  PubMed  Google Scholar 

  30. Y Kuroiwa P Kasinathan YJ Choi R Naeem K Tomizuka et al. (2002) ArticleTitleCloned transchromosomic calves producing human immunoglobulin. Nat Biotechnol 20 889–894 Occurrence Handle10.1038/nbt727 Occurrence Handle1:CAS:528:DC%2BD38Xms1ahsL0%3D Occurrence Handle12172556

    Article  CAS  PubMed  Google Scholar 

  31. I Kuzmin L Geil H Ge U Bengtsson FM Duh et al. (1999) ArticleTitleAnalysis of aberrant methylation of the VHL gene by transgenes, monochromosome transfer, and cell fusion. Oncogene 18 5672–5679 Occurrence Handle10.1038/sj.onc.1202959 Occurrence Handle1:CAS:528:DyaK1MXntVOis7c%3D Occurrence Handle10523847

    Article  CAS  PubMed  Google Scholar 

  32. JW Littlefield (1964) ArticleTitleSelection of hybrids from matings of fibroblasts in vitro and their presumed recombinants. Science 145 709–710 Occurrence Handle1:STN:280:CCuC38nisFE%3D Occurrence Handle14168277

    CAS  PubMed  Google Scholar 

  33. G Luo IM Santoro LD McDaniel I Nishijima M Mills et al. (2000) ArticleTitleCancer predisposition caused by elevated mitotic recombination in Bloom mice. Nat Genet 26 424–429 Occurrence Handle10.1038/82548 Occurrence Handle1:CAS:528:DC%2BD3cXptVWgtr4%3D Occurrence Handle11101838

    Article  CAS  PubMed  Google Scholar 

  34. T Mashimo G Goodarzi M Watabe AP Cuthbert RF Newbold et al. (2000) ArticleTitleLocalization of a novel tumor metastasis suppressor region on the short arm of human chromosome 2. Genes Chromosomes Cancer 28 285–293 Occurrence Handle10.1002/1098-2264(200007)28:3<285::AID-GCC6>3.0.CO;2-O Occurrence Handle1:CAS:528:DC%2BD3cXks1Skt7Y%3D Occurrence Handle10862034

    Article  CAS  PubMed  Google Scholar 

  35. S Matsuura C Weemaes D Smeets H Takami N Kondo et al. (1997) ArticleTitleGenetic mapping using microcell-mediated chromosome transfer suggests a locus for Nijmegen breakage syndrome at chromosome 8q21-24. Am J Hum Genet 60 1487–1494 Occurrence Handle1:CAS:528:DyaK2sXlsFCisb8%3D Occurrence Handle9199571

    CAS  PubMed  Google Scholar 

  36. LD McDaniel RA Schultz (1992) ArticleTitleElevated sister chromatid exchange phenotype of Bloom syndrome cells is complemented by human chromosome 15. Proc Natl Acad Sci USA 89 7968–7972 Occurrence Handle1:CAS:528:DyaK38XmtFSitLo%3D Occurrence Handle1518822

    CAS  PubMed  Google Scholar 

  37. CA McNeill RL Brown (1980) ArticleTitleGenetic manipulation by means of microcell-mediated transfer of normal human chromosomes into recipient mouse cells. Proc Natl Acad Sci USA 77 5394–5398 Occurrence Handle1:CAS:528:DyaL3cXmtFyltbg%3D Occurrence Handle7001478

    CAS  PubMed  Google Scholar 

  38. JE Mejia A Alazami A Willmott P Marschall E Levy et al. (2002) ArticleTitleEfficiency of de novo centromere formation in human artificial chromosomes. Genomics 79 297–304 Occurrence Handle10.1006/geno.2002.6704 Occurrence Handle1:CAS:528:DC%2BD38XhsVGgsrw%3D Occurrence Handle11863359

    Article  CAS  PubMed  Google Scholar 

  39. W Mills R Critcher C Lee CJ Farr (1999) ArticleTitleGeneration of an approximately 2.4 Mb human X centomere-based minichromosome by targeted telomere-associated chromosome fragmentation in DT40. Hum Mol Genet 8 751–761 Occurrence Handle1:CAS:528:DyaK1MXivFamsb0%3D Occurrence Handle10196364

    CAS  PubMed  Google Scholar 

  40. M Negrini S Sabbioni L Possati S Rattan A Corallini et al. (1994) ArticleTitleSuppression of tumorigenicity of breast cancer cells by microcell-mediated chromosome transfer: studies on chromosomes 6 and 11. Cancer Res 54 1331–1336 Occurrence Handle1:CAS:528:DyaK2cXitFCjtrw%3D Occurrence Handle8118824

    CAS  PubMed  Google Scholar 

  41. R Ramirez-Solis AC Davis A Bradley (1993) ArticleTitleGene targeting in embryonic stem cells. Methods Enzymol 225 855–878 Occurrence Handle1:CAS:528:DyaK2cXht1Kiurw%3D Occurrence Handle8231891

    CAS  PubMed  Google Scholar 

  42. SL Ricketts JC Carter WB Coleman (2003) ArticleTitleIdentification of three 11p11.2 candidate liver tumor suppressors through analysis of known human genes. Mol Carcinog 36 90–99 Occurrence Handle10.1002/mc.10101 Occurrence Handle1:CAS:528:DC%2BD3sXhtF2ksrw%3D Occurrence Handle12557265

    Article  CAS  PubMed  Google Scholar 

  43. NR Ringertz RE Savage (1976) Cell hybrids. Academic Press New York

    Google Scholar 

  44. P Rollini L Xu RE Fournier (1999) ArticleTitlePartial activation of gene activity and chromatin remodeling of the human 14q32.1 serpin gene cluster by HNF-1 alpha and HNF-4 in fibroblast microcell hybrids. Somat Cell Mol Genet 25 207–221 Occurrence Handle10.1023/A:1019279809728 Occurrence Handle1:CAS:528:DC%2BD3MXnt1GmtL0%3D Occurrence Handle11586788

    Article  CAS  PubMed  Google Scholar 

  45. R Saffery KH Choo (2002) ArticleTitleStrategies for engineering human chromosomes with therapeutic potential. J Gene Med 4 5–13 Occurrence Handle10.1002/jgm.236 Occurrence Handle11828382

    Article  PubMed  Google Scholar 

  46. JA Sanford E Stubblefield (1987) ArticleTitleGeneral protocol for microcell-mediated chromosome transfer. Somat Cell Mol Genet 13 279–284 Occurrence Handle1:CAS:528:DyaL2sXkslekurk%3D Occurrence Handle3299749

    CAS  PubMed  Google Scholar 

  47. PJ Saxon ES Srivatsan GV Leipzig JH Sameshima EJ Stanbridge (1985) ArticleTitleSelective transfer of individual human chromosomes to recipient cells. Mol Cell Biol 5 140–146 Occurrence Handle1:CAS:528:DyaL2MXhtVOns7w%3D Occurrence Handle3982414

    CAS  PubMed  Google Scholar 

  48. SL Schor RT Johnson AM Mullinger (1975) ArticleTitlePerturbation of mammalian cell division. II. Studies on the isolation and characterization of human mini segregant cells. J Cell Sci 19 281–303 Occurrence Handle1:STN:280:CSmD28blsVQ%3D Occurrence Handle53236

    CAS  PubMed  Google Scholar 

  49. MJ Seraj RS Samant MF Verderame DR Welch (2000) ArticleTitleFunctional evidence for a novel human breast carcinoma metastasis suppressor, BRMS1, encoded at chromosome 11q13. Cancer Res 60 2764–2769 Occurrence Handle1:CAS:528:DC%2BD3cXjvFOls7g%3D Occurrence Handle10850410

    CAS  PubMed  Google Scholar 

  50. A Seyda RF Newbold TJ Hudson A Verner N MacKay et al. (2001) ArticleTitleA novel syndrome affecting multiple mitochondrial functions, located by microcell-mediated transfer to chromosome 2p14-2p13. Am J Hum Genet 68 386–396 Occurrence Handle10.1086/318196 Occurrence Handle1:CAS:528:DC%2BD3MXhtl2lsL0%3D Occurrence Handle11156534

    Article  CAS  PubMed  Google Scholar 

  51. T Shinohara K Tomizuka S Miyabara S Takehara Y Kazuki et al. (2001) ArticleTitleMice containing a human chromosome 21 model behavioral impairment and cardiac anomalies of Down’s syndrome. Hum Mol Genet 10 1163–1175 Occurrence Handle10.1093/hmg/10.11.1163 Occurrence Handle1:CAS:528:DC%2BD3MXktlSlt7w%3D Occurrence Handle11371509

    Article  CAS  PubMed  Google Scholar 

  52. O Smithies RG Gregg SS Boggs MA Koralewski RS Kucherlapati (1985) ArticleTitleInsertion of DNA sequences into the human chromosomal beta-globin locus by homologous recombination. Nature 317 230–234 Occurrence Handle1:CAS:528:DyaL2MXmtVyjtr8%3D Occurrence Handle2995814

    CAS  PubMed  Google Scholar 

  53. RD Steenbergen D Kramer CJ Meijer JM Walboomers DA Trott et al. (2001) ArticleTitleTelomerase suppression by chromosome 6 in a human papillomavirus type 16-immortalized keratinocyte cell line and in a cervical cancer cell line. J Natl Cancer Inst 93 865–872 Occurrence Handle10.1093/jnci/93.11.865 Occurrence Handle1:CAS:528:DC%2BD3MXltVOhu7c%3D Occurrence Handle11390536

    Article  CAS  PubMed  Google Scholar 

  54. K Tomizuka H Yoshida H Uejima H Kugoh K Sato et al. (1997) ArticleTitleFunctional expression and germline transmission of a human chromosome fragment in chimaeric mice. Nat Genet 16 133–143 Occurrence Handle1:CAS:528:DyaK2sXjs1aiurw%3D Occurrence Handle9171824

    CAS  PubMed  Google Scholar 

  55. K Tomizuka T Shinohara H Yoshida H Uejima A Ohguma et al. (2000) ArticleTitleDouble trans-chromosomic mice: maintenance of two individual human chromosome fragments containing Ig heavy and kappa loci and expression of fully human antibodies. Proc Natl Acad Sci USA 97 722–727 Occurrence Handle10.1073/pnas.97.2.722 Occurrence Handle1:CAS:528:DC%2BD3cXot1agsw%3D%3D Occurrence Handle10639146

    Article  CAS  PubMed  Google Scholar 

  56. R van Coster L De Meirleir (2000) ArticleTitleMitochondrial cytopathies and neuromuscular disorders. Acta Neurol Belg 100 156–161 Occurrence Handle1:STN:280:DC%2BD3MzgtlahsQ%3D%3D Occurrence Handle11098288

    CAS  PubMed  Google Scholar 

  57. G Veomett DM Prescott J Shay KR Porter (1974) ArticleTitleReconstruction of mammalian cells from nuclear and cytoplasmic components separated by treatment with cytochalasin B. Proc Natl Acad Sci USA 71 1999–2002 Occurrence Handle1:STN:280:CSuB28%2FptlI%3D Occurrence Handle4525471

    CAS  PubMed  Google Scholar 

  58. MC Weiss H Green (1967) ArticleTitleHuman-mouse hybrid cell lines containing partial complements of human chromosomes and functioning human genes. Proc Natl Acad Sci USA 58 1104–1111 Occurrence Handle1:STN:280:CCeD3M3jtlY%3D Occurrence Handle5233838

    CAS  PubMed  Google Scholar 

  59. D Wohrle U Salat H Hameister W Vogel P Steinbach (2001) ArticleTitleDemethylation, reactivation, and destabilization of human fragile X full-mutation alleles in mouse embryocarcinoma cells. Am J Hum Genet 69 504–515 Occurrence Handle10.1086/322739 Occurrence Handle1:CAS:528:DC%2BD3MXmvV2kurc%3D Occurrence Handle11462172

    Article  CAS  PubMed  Google Scholar 

  60. I Yoshida Y Nishita TK Mohandas N Takagi (1997) ArticleTitleReactivation of an inactive human X chromosome introduced into mouse embryonal carcinoma cells by microcell fusion with persistent expression of XIST. Exp Cell Res 230 208–219 Occurrence Handle10.1006/excr.1996.3393 Occurrence Handle1:CAS:528:DyaK2sXhtVShtb4%3D Occurrence Handle9024780

    Article  CAS  PubMed  Google Scholar 

  61. H Yoshioka Y Shirayoshi M Oshimura (2001) ArticleTitleA novel in vitro system for analyzing parental allele-specific histone acetylation in genomic imprinting. J Hum Genet 46 626–632 Occurrence Handle10.1007/s100380170013 Occurrence Handle1:CAS:528:DC%2BD3MXoslWhurw%3D Occurrence Handle11721881

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

AOD is funded by the Wellcome Trust. We thank Frank Ruddle, Stephen Goss, Lee Silver, Jonathan Halliwell, and Victor Tybulewicz for helpful comments on this manuscript. We thank Sandra Ruf for assistance with mouse photos, Holger Hummerich and Al Necal for bioinformatics analysis, and Ray Young for graphics. We are using the notation ‘Hsa1, Hsa2...HsaX’ to denote human chromosomes (Homo sapiens).

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  1. Department of Neurodegenerative Disease, Institute of Neurology, Queen Square, London WC1N 3BG, UK

    Aideen M. O. Doherty & Elizabeth M. C. Fisher

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  1. Aideen M. O. Doherty
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Correspondence to Aideen M. O. Doherty.

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Doherty, A.M., Fisher, E.M. Microcell-mediated chromosome transfer (MMCT): small cells with huge potential . Mamm Genome 14, 583–592 (2003). https://doi.org/10.1007/s00335-003-4002-0

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