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Metabolic fingerprinting of arctic copepods Calanus finmarchicus, Calanus glacialis and Calanus hyperboreus

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Abstract

Recent development in high-throughput unbiased analytical tools (transcriptomics, proteomics and metabolomics) has opened the possibility to assess a multitude of molecular endpoints in organisms. In the present work, we used a combination of metabolomics tools, proton nuclear magnetic resonance spectroscopy (1H-NMR) and liquid chromatography coupled with mass spectrometry (LC–MS),) and multivariate statistical analysis to identify species-specific metabolome fingerprints and species-specific metabolites in the three Arctic copepods Calanus finmarchicus, C. glacialis and C. hyperboreus. Principal component analysis separated the three species with high specificity and sensitivity, and some species-specific metabolites were putatively annotated. These tools can be used for future studies within basal biology, systems biology, bioprospecting and ecotoxicology. As a supplementary analytical tool to genetic analyses, species-specific metabolites have a potential to be used to separate closely related Arctic Calanus species from net hauls.

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Acknowledgments

This project was financed by the SINTEF internal fund (Konsernsatsning Calanomics) and the Research Council of Norway (Grant 196711/S40). The field expedition to Svalbard was financed by Svalbard Science Forum.

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Authors and Affiliations

  1. SINTEF Materials and Chemistry, Marine Environmental Technology, 7465, Trondheim, Norway

    Bjørn Henrik Hansen, Ida Beathe Øverjordet & Trond R. Størseth

  2. SINTEF Materials and Chemistry, Biotechnology, 7465, Trondheim, Norway

    Kristin Degnes

  3. BioTrix, 7022, Trondheim, Norway

    Dag Altin

Authors
  1. Bjørn Henrik Hansen
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  2. Kristin Degnes
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  3. Ida Beathe Øverjordet
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  4. Dag Altin
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  5. Trond R. Størseth
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Correspondence to Bjørn Henrik Hansen.

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Hansen, B.H., Degnes, K., Øverjordet, I.B. et al. Metabolic fingerprinting of arctic copepods Calanus finmarchicus, Calanus glacialis and Calanus hyperboreus . Polar Biol 36, 1577–1586 (2013). https://doi.org/10.1007/s00300-013-1375-8

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  • DOI: https://doi.org/10.1007/s00300-013-1375-8

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