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cDNA microarray analysis reveals the capacity of the cold-adapted Antarctic fish Trematomus bernacchii to alter gene expression in response to heat stress

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Abstract

During their many millions of years of evolution in the extreme and stable cold, Antarctic notothenioid fishes have acquired profoundly cold-adapted physiologies. Gene expression profiling via cDNA microarray was used to determine the extent to which one species of notothenioid, Trematomus bernacchii, has retained the ability to alter gene expression in response to heat stress. While an inability to up-regulate the expression of any size class of heat shock proteins (except for a 1.1-fold induction of the co-chaperone Hsp40) was observed, hundreds of additional genes, associated with a broad range of cellular processes, were responsive to heat. Many of these genes are associated with central aspects of the evolutionarily conserved cellular stress response (CSR), which plays a pivotal role in responding to physical and chemical stresses. The inability of T. bernacchii to mount a heat shock response underscores the potential susceptibility of this species to the effects of global warming.

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Acknowledgments

This work was supported by NSF-OPP Fellowship Award 0443754 to BAB and NSF-OPP Award OPP05-04072 to Dr. Donal Manahan for the Antarctic Biology Course. Our appreciation goes to Dr. Gretchen Hofmann and the members of B-134 for field assistance.

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Authors and Affiliations

  1. Department of Biology, Portland State University, Portland, OR, 97207, USA

    Bradley A. Buckley

  2. Hopkins Marine Station, Stanford University, Pacific Grove, CA, 93950, USA

    George N. Somero

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  1. Bradley A. Buckley
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Correspondence to Bradley A. Buckley.

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Buckley, B.A., Somero, G.N. cDNA microarray analysis reveals the capacity of the cold-adapted Antarctic fish Trematomus bernacchii to alter gene expression in response to heat stress. Polar Biol 32, 403–415 (2009). https://doi.org/10.1007/s00300-008-0533-x

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  • DOI: https://doi.org/10.1007/s00300-008-0533-x

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