Abstract
The presence, concentration and distribution of the iron regulated proteins, ferredoxin and flavodoxin, was investigated in pack ice off eastern Antarctica using SDS-PAGE gels. Bands corresponding to ferredoxin and/or flavodoxin were observed in all but eight of the 102 core sections analysed. Flavodoxin was found in most of the ice samples and was strongly correlated with chlorophyll a standing stock. The widespread distribution of flavodoxin here is not thought to indicate iron-limitation as many of the dominant species, such as Fragilariopsis cylindrus, Cylindrotheca closterium, are known to produce this protein under iron-replete conditions and thus the significant correlation between flavodoxin and biomass is likely to be the result of widespread constitutive flavodoxin expression among the diatoms that commonly inhabit sea ice. High concentrations of ferredoxin were predominantly derived from core sections on the floes closest to the continent and also in the upper portion of these floes. There was a consistent lack of ferredoxin expression in the high biomass bottom communities. The absence of ferredoxin is likely to indicate a reduced supply of iron but the significance of this reduced iron supply cannot be inferred on the basis of protein expression alone. Furthermore, in the present study the observed variability in the flavodoxin:ferredoxin ratio may not simply reflect the iron nutritional status of the community, but probably results from changes in the abundance of species capable of expressing ferredoxin.
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Arar EJC (1997) In vitro determination of chlorophyll and pheophytin a in marine and freshwater algae by fluorescence. National Exposure Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, Cincinnati, Ohio
Arrigo KR (2003) Primary production in sea ice. In: Thomas DN, Dieckmann G (eds) Sea ice: an introduction to its physics, chemistry, biology and geology. Blackwell Science, Oxford, pp 143–183
Arrigo KR, Robinson DH, Dunbar RB, Leventer AR, Lizotte MP (2003) Physical control of chlorophyll a, POC, and TPN distributions in the pack ice of the Ross Sea, Antarctica. J Geophys Res Oceans, 108 (C10), Art. No. 3316
Bindoff NL, Rosenberg MA, Warner MJ (2000) On the circulation and water masses over the Antarctic continental slope and rise between 80 and 150°E. Deep Sea Res Part II Top Stud Oceanogr 47:2299–2326
Bindoff NL, Williams GD, Allison I (2001) Sea-ice growth and water-mass modification in the Mertz Glacier polynya, East Antarctica, during winter. Ann Glaciol 3:399–406
Blain S, Queguiner B, Armand L, Belviso S, Bombled B, Bopp L, Bowie A, Brunet C, Brussaard C, Carlotti F, Christaki U, Corbiere A, Durand I, Ebersbach F, Fuda J-L, Garcia N, Gerringa L, Griffiths B, Guigue C, Guillerm C, Jacquet S, Jeandel C, Laan P, Lefevre D, Monaco C, Malits A, Mosseri J, Obernosterer I, Park Y-H, Picheral M, Pondavan P, Renmeyi T, Sandroni V, Sarthou G, Savoye N, Scouarnec L, Souhaut M, Thuiller D, Timmermans K, Trull T, Uitz J, van Beek P, Veldhuis M, Invent D, Viollier E, Vong L, Wagener T (2007) Effect of natural iron fertilization on carbon sequestration in the Southern Ocean. Nature 446:1070–1075
Boyd PW (2002a) Environmental factors controlling phytoplankton processes in the Southern Ocean. J Phycol 38:844–861
Boyd PW (2002b) The role of iron in the biogeochemistry of the Southern Ocean and equatorial Pacific: a comparison of in situ iron enrichments. Deep Sea Res Part II Top Stud Oceanogr 49:1803–1821
Boyd P, LaRoche J, Gall M, Frew R, McKay RML (1999) Role of iron, light and silicate in controlling algal biomass in subantarctic waters SE of New Zealand. J Geophys Res Oceans 104(C6):13395–13408
Boyd PW, Watson AJ, Law CS, Abraham ER, Trull T, Murdoch R, Bakker DCE, Bowie AR, Buesseler KO, Chang H, Charette M, Croot P, Downing K, Frew R, Gall M, Hadfield M, Hall J, Harvey M, Jameson G, LaRoche J, Liddicoat M, Ling R, Maldonado MT, McKay RM, Nodder S, Pickmere S, Pridmore R, Rintoul S, Safi K, Sutton P, Strzepek R, Tanneberger K, Turner S, Waite A, Zeldis J (2000) A mesoscale phytoplankton bloom in the polar Southern Ocean stimulated by iron fertilization. Nature 407:695–702
Boyd PW, Jickells T, Law CS, Blain S, Boyle EA, Buesseler KO, Coale KH, Cullen JJ, de Baar HJW, Follows M, Harvey M, Lancelot C, Levasseur M, Owens NPJ, Pollard R, Rivkin RB, Sarmiento J, Schoemann V, Smetacek V, Takeda S, Tsuda S, Turner S, Watson AJ (2007) Mesoscale Iron enrichment experiments 1993–2005: synthesis and future directions. Science 315:612–617
Coale KH, Johnson KS, Chavez FP, Buesseler KO, Barber RT, Brzezinski MA, Cochlan WP, Millero FJ, Falkowski PG, Bauer JE, Wanninkhof RH, Kudela RM, Altabet MA, Hales BE, Takahashi T, Landry MR, Bidigare R, Wang XJ, Chase Z, Strutton PG, Friederich GE, Gorbunov MY, Lance VP, Hilting AK, Hiscock MR, Demarest M, Hiscock WT, Sullivan KF, Tanner SJ, Gordon RM, Hunter CN, Elrod VA, Fitzwater SE, Jones JL, Tozzi S, Koblizek M, Roberts AE, Herndon J, Brewster J, Ladizinsky N, Smith G, Cooper D, Timothy D, Brown SL, Selph KE, Sheridan CC, Twining BS, Johnson ZI (2004) Southern Ocean iron enrichment experiment: carbon cycling in high- and low-Si waters. Science 304:408–414
Coale KH, Gordon RM, Wang XJ (2005) The distribution and behavior of dissolved and particulate iron and zinc in the Ross Sea and Antarctic circumpolar current along 170°W. Deep Sea Res Part II Top Stud Oceanogr 52:295–318
Comiso JC (2003) Large-scale variability of the global sea ice cover. In: Thomas DN, Dieckmann G (eds) Sea ice: an introduction to its physics, chemistry, biology and geology. Blackwell Science, Oxford, pp 112–142
Croot PL, Andersson K, Ozturk M, Turner DR (2004) The distribution and specification of iron along 6°E in the Southern Ocean. Deep Sea Res Part II Top Stud Oceanogr 51:2857–2879
de Baar HJW, Boyd PM (2000) The role of iron in plankton ecology and carbon dioxide transfer of the global oceans. In: Hanson RB, Ducklow HW, Hunter KA (eds) The biogeochemistry of iron in seawater. John Wiley, Chichester, pp 123–253
de Baar HJW, de Jong JTM, Bakker DCE, Loscher BM, Veth C, Bathmann U, Smetacek V (1995) Importance of iron for plankton blooms and carbon-dioxide drawdown in the Southern-Ocean. Nature 373:412–415
de Baar HJW, de Jong JTM, Nolting RF, Timmermans KR, van Leeuwe MA, Bathmann U, van der Loeff MR, Sildam J (1999) Low dissolved Fe and the absence of diatom blooms in remote Pacific waters of the Southern Ocean. Mar Chem 66:1–34
Doucette GJ, Erdner DL, Peleato ML, Hartman JL, Anderson DM (1996) Quantitative analysis of iron-stress related proteins in Thalassiosira weissflogii: measurement of flavodoxin and ferredoxin using HPLC. Mar Ecol Prog Ser 130:269–276
Edwards R, Sedwick P (2001) Iron in East Antarctic snow: implications for atmospheric iron deposition and algal production in Antarctic waters. Geophys Res Lett 28:3907–3910
Eicken H (1992) The role of sea ice in structuring Antarctic ecosystems. Polar Biol 12:3–13
Eicken H (2003) From the microscopic, to the macroscopic, to the regional scale: growth, microstructure and properties of sea ice. In: Thomas DN, Dieckmann G (eds) Sea ice: an introduction to its physics, chemistry, biology and geology. Blackwell Science, Oxford, pp 22–81
Erdner DL, Anderson DM (1999) Ferredoxin and flavodoxin as biochemical indicators of iron limitation during open-ocean iron enrichment. Limnol Oceanogr 44:1609–1615
Erdner DL, Price NM, Doucette GJ, Peleato ML, Anderson DM (1999) Characterization of ferredoxin and flavodoxin as markers of iron limitation in marine phytoplankton. Mar Ecol Prog Ser 184:43–53
Fitzwater SE, Johnson KS, Gordon RM, Coale KH, Smith WO (2000) Trace metal concentrations in the Ross Sea and their relationship with nutrients and phytoplankton growth. Deep Sea Res Part II Top Stud Oceanogr 47:3159–3179
Gervais F, Riebesell U, Gorbunov MY (2002) Changes in primary productivity and chlorophyll a in response to iron fertilization in the Southern Polar Frontal Zone. Limnol Oceanogr 47:1324–1335
Glover H (1977) Effects of iron deficiency on Isochrysis galbana (Chrysophyceae) and Phaeodactylum tricornutum (Bacillariophyceae). J. Phycol 13:208–212
Grose M, McMinn A (2003) Algal biomass in east Antarctic pack ice: how much is in the east? In: Huiskies AHL, Gieskes WWC, Rozema J, Schorno RML, van der Vies SM, Wolff WJ (eds) Antarctic biology in a global context. Backhuys, Leiden, pp 21–25
Kristiansen S, Farbrot T, Kuosa H, Myklestad S, Quillfeldt CHV (1998) Nitrogen uptake in the infiltration community, an ice algal community in Antarctic pack-ice. Polar Biol 19:307–315
Lannuzel D, Schoemann V, de Jong J, Tison J-L, Chou L (2007) Distribution and biogeochemical behavoir of iron in the East Antarctic sea ice. Mar Chem 106:18–32
La Roche J, Murray H, Orellana M, Newton J (1995) Flavodoxin expression as an indicator of iron limitation in marine diatoms. J Phycol 31:520–530
La Roche J, Boyd PW, McKay RML, Geider RJ (1996) Flavodoxin as an in situ marker for iron stress in phytoplankton. Nature 382:802–805
Loscher BM, de Baar HJW, de Jong JTM, Veth C, Dehairs F (1997) The distribution of Fe in the Antarctic circumpolar current. Deep Sea Res Part II Top Stud Oceanogr 44:143–187
Maldonado MT, Price NM (2001) Reduction and transport of organically bound iron by Thalassiosira oceanica (Bacillariophyceae). J Phycol 37:298–309
Martin JH, Fitzwater SE (1988) Iron-deficiency limits phytoplankton growth in the Northeast Pacific subarctic. Nature 331:341–343
Martin JH, Gordon RM, Fitzwater SE (1990) Iron in Antarctic waters. Nature 345:156–158
McKay RML, Geider RJ, LaRoche J (1997) Physiological and biochemical response of the photosynthetic apparatus of two marine diatoms to Fe stress. Plant Physiol 114:615–622
McKay RML, La Roche J, Yakunin AF, Durnford DG, Geider RJ (1999) Accumulation of ferredoxin and flavodoxin in a marine diatom in response to Fe. J Phycol 35:510–519
McKay RML, Villareal TA, La Roche J (2000) Vertical migration by Rhizosolenia sp. (Bacillariophyceae): implications for Fe acquisition. J Phycol 36:669–674
McMinn A, Ryan KG, Ralph P, Pankowski A (2007) Spring sea ice photosynthesis, primary productivity and biomass distribution in eastern Antarctica, 2002–2004. Mar Biol 151:985–995
Measures CI, Vink S (2001) Dissolved Fe in the upper waters of the Pacific sector of the Southern Ocean. Deep Sea Res Part II Top Stud Oceanogr 48:3913–3941
Mock T (2002) In situ primary production in young Antarctic sea ice. Hydrobiol 470:127–132
Moore CM, Seeyava S, Hickman AE, Allen JT, Luca MI, lanquette H, Pollard RT, Poulton AJ (2007) Iron–light interactions during the CROZet natural iron bloom and export experiment (CROZEX) I: phytoplankton growth and physiology. Deep Sea Res Part II Top Stud Oceaonogr 54:2045–2065
Porra RJ, Thompson WA, Kriedemann PE (1989) Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorbtion spectroscopy. Biochim Biophys Acta 975:384–394
Sambrotto RN, Matsuda A, Vaillancourt R, Brown M, Langdon C, Jacobs SS, Measures C (2003) Summer plankton production and nutrient consumption patterns in the Mertz Glacier Region of East Antarctica. Deep Sea Res Part II Top Stud Oceanogr 50:1393–1414
Sedwick PN, DiTullio GR (1997) Regulation of algal blooms in Antarctic shelf waters by the release of iron from melting sea ice. Geophys Res Lett 24:2515–2518
Sedwick PN, DiTullio GR, Mackey DJ (2000) Iron and manganese in the Ross Sea, Antarctica: seasonal iron limitation in Antarctic shelf waters. J Geophys Res Oceans 105:11321–11336
Sohrin Y, Iwamoto S, Matsui M, Obata H, Nakayama E, Suzuki K, Handa N, Ishii M (2000) The distribution of Fe in the Australian sector of the Southern Ocean. Deep Sea Res Part I Oceanogr Res Pap 47:55–84
Strzepek RF, Price NM (2000) Influence of irradiance and temperature on the iron content of the marine diatom Thalassiosira weissflogii (Bacillariophyceae). Mar Ecol Prog Ser 206:107–117
Sunda WG, Huntsman SA (1997) Interrelated influence of iron, light and cell size on marine phytoplankton growth. Nature 390:389–392
Syvertsen EE, Kristiansen S (1993) Ice Algae During Epos, Leg–1: assemblages, biomass, origin and nutrients. Polar Biol 13:61–65
Thomas DN (2003) Iron limitation in the Southern Ocean. Science 302:565–565
Thomas DN, Papadimitriou S (2003) Biogeochemistry of sea ice. In: Thomas DN, Dieckmann G (eds) Sea ice: an introduction to its physics, chemistry, biology and geology. Blacwell Science, Oxford, pp 267–302
Timmermans KR, van Leeuwe MA, de Jong JTM, McKay RML, Nolting RF, Witte HJ, van Ooyen J, Swagerman MJW, Kloosterhuis H, de Baar HJW (1998) Iron stress in the Pacific region of the Southern Ocean: evidence from enrichment bioassays. Mar Ecol Prog Ser 166:27–41
Trevena AJ, Jones GB, Wright SW, van den Enden RL (2000) Profiles of DMSP, algal pigments, nutrients and salinity in pack ice from eastern Antarctica. J Sea Res 43:265–273
Trull T, Rintoul SR, Hadfield M, Abraham ER (2001) Circulation and seasonal evolution of polar waters south of Australia: implications for iron fertilization of the Southern Ocean. Deep Sea Res Part II Top Stud Oceanogr 48:2439–2466
van Oijen T, van Leeuwe MA, Gieskes WWC, de Baar HJW (2004) Effects of iron limitation on photosynthesis and carbohydrate metabolism in the Antarctic diatom Chaetoceros brevis (Bacillariophyceae). Eur J Phycol 39:161–171
Veldhuis M, de Baar HJW (2005) Iron resources and oceanic nutrients: advancement of global environment simulations. J Sea Res 53:1–6
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Pankowski, A., McMinn, A. Ferredoxin and flavodoxin in eastern Antarctica pack ice. Polar Biol 31, 1153–1165 (2008). https://doi.org/10.1007/s00300-008-0451-y
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DOI: https://doi.org/10.1007/s00300-008-0451-y