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Absence of amylose in sweet potato [Ipomoea batatas (L.) Lam.] following the introduction of granule-bound starch synthase I cDNA

  • Physiology and Biochemistry
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Abstract.

The full-length sense cDNA for sweet potato granule-bound starch synthase I (GBSSI) driven by the CaMV 35S promoter was introduced into the sweet potato by Agrobacterium tumefaciens-mediated transformation. Out of the 26 transgenic plants obtained, one plant showed the absence of amylose in the tuberous root as determined by the iodine colorimetric method. Electrophoresis analysis failed to detect the GBSSI protein, suggesting that gene silencing of the GBSSI gene occurred in the transgenic sweet potato plant. These results demonstrate that starch composition in the tuberous root of sweet potato can be altered by genetic transformation.

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Author notes

  1. T. Kimura & A. Saito

    Present address: National Agricultural Research Center for Kyushu Okinawa Region, 2421 Suya, Nishigoshi, Kumamoto, 861-1192, Japan

  2. T. Noda

    Present address: Department of Upland Agriculture, National Agricultural Research Center for Hokkaido Region, Shinsei, Memuro, Hokkaido, 082-0071, Japan

  3. O. Ideta

    Present address: National Institute of Crop Science, 2-1-18, Kannondai, Tsukuba, 305-8518, Japan

Authors and Affiliations

  1. Kyushu National Agricultural Experiment Station, 2421 Suya, Nishigoshi, Kumamoto, 861-1192, Japan

    T. Kimura, T. Noda & A. Saito

  2. Research Institute of Agricultural Resources, Ishikawa Agricultural College, Nonoichi, Ishikawa, 921-8836, Japan

    M. Otani & T. Shimada

  3. Japan International Research Center for Agricultural Sciences, Okinawa Subtropical Station, Ishigaki, Okinawa, 907-0002, Japan

    O. Ideta

Authors
  1. T. Kimura
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  2. M. Otani
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  3. T. Noda
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  4. O. Ideta
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  5. T. Shimada
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  6. A. Saito
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Revision received: 25 June 2001

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Kimura, T., Otani, M., Noda, T. et al. Absence of amylose in sweet potato [Ipomoea batatas (L.) Lam.] following the introduction of granule-bound starch synthase I cDNA. Plant Cell Rep 20, 663–666 (2001). https://doi.org/10.1007/s002990100376

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  • DOI: https://doi.org/10.1007/s002990100376

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