Abstract
Microprojectile-mediated transient and integrative transformation frequencies in rice (Oryza sativa cv. Taipei 309) embryogenic suspension cells were studied as a function of various parameters. Mannitol at concentrations of 0.5 and 0.6 m was best for osmotic preconditioning of the cells for transient, but not for integrative transformation, for which sucrose yielded the best and most reliable results. Denaturation of the transforming plasmid DNA prior to bombardment improved transient and integrative transformation frequencies two to three fold. Delivery of double-stranded plasmids in linear form had no effect on transient transformation when compared to supercoiled plasmid DNA, but led to an overall two fold increase in integrative transformation frequency. This shows that optimized protocols for generating transgenic plants should not be based exclusively on transient gene expression assays.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 29 September 1997 / Revision received: 27 February 1998 / Accepted: 2 April 1998
Rights and permissions
About this article
Cite this article
Nandadeva, Y., Lupi, C., Meyer, C. et al. Microprojectile-mediated transient and integrative transformation of rice embryogenic suspension cells: effects of osmotic cell conditioning and of the physical configuration of plasmid DNA. Plant Cell Reports 18, 500–504 (1999). https://doi.org/10.1007/s002990050611
Issue Date:
DOI: https://doi.org/10.1007/s002990050611