Abstract.
Inducible promoters or gene-switches are used to both spatially and temporally regulate gene expression. Such regulation can provide information concerning the function of a gene in a developmental context as well as avoid potential harmful effects due to overexpression. A gfp construct under the control of a copper-inducible promoter was introduced into Arabidopsis thaliana (L.) Heynh. and the regulatory parameters of this inducible promoter were determined. Here, we describe the time-course of up- and down-regulation of GFP expression in response to copper level, the optimal regulatory levels of copper, and the tissue specificity of expression in three transgenic lines. We conclude that the copper-inducible promoter system may be useful in regulating the time and location of gene expression in A. thaliana.
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Revision received: 20 November 2000
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Granger, C., Cyr, R. Characterization of the yeast copper-inducible promoter system in Arabidopsis thaliana. Plant Cell Reports 20, 227–234 (2001). https://doi.org/10.1007/s002990000308
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DOI: https://doi.org/10.1007/s002990000308