Cauliflower somatic embryogenesis and analysis of regenerant stability by ISSRs

Abstract

  To study the somatic embryogenesis of Brassica oleracea var. botrytis L., hypocotyls were placed on Murashige and Skoog's medium (1962) with 1 mg.l^–1 of 2,4-dichlorophenoxyacetic acid and 1 mg.l^–1 of kinetin to induce callogenesis. After transfer of the calli to the maturation medium, somatic embryos appeared. They developed into plantlets and the potential of regeneration of the calli was maintained for more than 8 months. Thirty-five plantlets were produced after 2 months of culture, then transplanted into soil. Inter-simple sequence repeat markers generated by trinucleotidic and tetranucleotidic primers were tested for their ability to characterise genomic variations in the obtained plants. The absence of polymorphism between different regenerants from the same cultivar indicates the conformity of the regeneration protocol.